Genetic variability among<i>Dicrocoelium dendriticum</i>isolates from different regions in Shaanxi Province, China revealed by sequences of three mitochondrial genes

Zhao, Guang‐Hui; Bian, Qingqing; Ren, Wan-Xin; Jia, Yanqing; Cheng, Wen-Yu; Fang, Yong; Song, Jun-Ke; Lin, Qing

doi:10.3109/19401736.2013.772168

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…However, low-level sequencing variations in mtDNA cox1, nad1, and cytb were discovered in D . dendriticum samples from various locations in Shaanxi Province, northern China [ 41 ]. In another study, the cox1 and nad1 fragments were used to reveal the intra-population genetic variations of D. chinensis from domestic yaks in Gansu and Sichuan Provinces, revealing lower intra-population genetic variations (1%), but higher inter-species differences (>10%) among common trematodes [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Validation of deep amplicon sequencing of Dicrocoelium in small ruminants from Northern regions of Pakistan

Khan,

Afshan,

Firasat

et al. 2024

PLoS ONE

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Dicrocoelium lancet flukes cause significant production loss in ruminant livestock. Although co-infection with multiple Dicrocoelium species within a host is common, techniques for studying the composition of these complex parasite communities are lacking. The pathogenicity, epidemiology, and therapeutic susceptibility of different helminth species vary, and little is known about the interactions that take place between co-infecting species and their hosts. Here, we describe the first applicationof metabarcoding deep amplicon sequencing method to studythe Dicrocoelium species in sheep and goats. First, rDNA ITS-2 sequences of four Dicrocoelium species (Dicrocoelium dendriticum, Dicrocoelium hospes, Dicrocoelium orientalis, and Dicrocoelium chinensis) were extracted from the NCBI public database. Phylogenetic analysis revealed separate clades of Dicrocoelium species; hence, molecular differentiation between each species is possible in co-infections. Second, 202 flukes belonging to seventeen host populations (morphologically verified as belonging to the Dicrocoelium genus) were evaluated to determine the deep amplicon sequencing read threshold of an individual fluke for each of the four species. The accuracy of the method in proportional quantification of samples collected from single hosts was further assessed. Overall, 198 (98.01%) flukes were confirmed as D. dendriticum and 1.98% produced no reads. The comparison of genetic distances between rDNA ITS-2 revealed 86% to 98% identity between the Dicrocoelium species. Phylogenetic analysis demonstrated a distinct clustering of species, apart from D. orientalis and D. chinensis, which sit very close to each other in a single large clade whereas D. hospes and D. dendriticum are separated into their own clade. In conclusion each sample was identified as D. dendriticum based on the proportion of MiSeq reads and validated the presence of this group of parasites in the Gilgit Baltistan and Khyber Pakhtunkhwa provinces of Pakistan. The metabarcoding deep amplicon sequencing technology and bioinformatics pathway have several potential applications, including species interactions during co-infections, identifying the host and geographical distribution of Dicrocoelium in livestock, drug therapy response evaluation and understanding of the emergence and spread of drug resistance.

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Section: Resultsmentioning

confidence: 99%

Validation of deep amplicon sequencing of Dicrocoelium in small ruminants from Northern regions of Pakistan

Khan,

Afshan,

Firasat

et al. 2024

PLoS ONE

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“…Several studies distinguished the Dicrocoelium spp. based on mtDNA, including D. chinensis and D. dendriticum ( 46 – 48 ). So far, no Cox1 analysis of Dicrocoelium species was conducted in the country and the present study is the first of its type to achieve such experimental results.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection and Identification of Fasciola spp. and Dicrocoelium spp. Isolated from the Ruminant Livestock of Northwest Iran Using High-Resolution Melting Analysis (HRM)

Hajialilo

Hosseini-Safa

Spotin

et al. 2023

ijph

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Background: The liver flukes of the Fasciola species and Dicrocoelium spp. are recognised as parasites of domestic and wild herbivores. Both species of F. hepatica and F. gigantica as well as D. dendriticum are distributed in Iran. The present study aimed to identify Fasciola spp. and Dicrocoelium spp. using mitochondrial Cox1 (cytochrome c oxidase I) gene by HRM method. Methods: Totally, thirty infected liver specimens were collected from the sheep (n:23) and cattle (n:7) at the abattoirs of Qazvin Province, northwest Iran in 2022. DNA extraction and PCR amplification of Cox1 gene were conducted by HRM technique. DnaSP v.5.0 was used for compression of diversity indices of ribosomal 28S rDNA and mitochondrial Cox1 markers of Dicrocoelium spp. The taxonomic status of Dicrocoelium spp. was performed by sequencing and phylogenetic analysis. Results: Overall, 26 and 4 isolates were identified as F. hepatica and F. gigantica, respectively. D. dendriticum was the sole infecting species of Dicrocoelium revealed by HRM analysis. Genomic analysis showed a moderate (28S rDNA genes: 0.600±0.215) to high (Cox1: 0.733±0.155) haplotype diversity for D. dendriticum. Conclusion: The parasite-dependent mitochondrial gene (Cox1) could identify a higher genetic diversity of D. dendriticum compared to nuclear 28S rDNA gene. HRM technique in the present study found to be a reliable technique for identification and genetic diversity of liver flukes but more comprehensive and in-depth studies in different parts of the country are needed.

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“…Dicrocoeliosis, 'the lancet fluke' is a world-wide parasitic disease that caused by Dicrocoelium spp. This parasite is an important hepatic trematode, affecting domestic and wild ruminant worldwide [1,2]. Dicrocoelium is found in the bile ducts and gall bladder of domestic and wild ruminants [1,3] .…”

Section: Introductionmentioning

confidence: 99%

“…This disease causes high direct and indirect economic losses in the livestock industry in Iran and other affected countries. The direct losses are caused by liver condemnation and the indirect losses are associated with the reduced meat and milk production, as well as, the costs associated with anthelminthic treatments [2,8,9] . Different studies have been conducted to determine the prevalence of Dicrocoeliosis in domestic ruminants, which all have reported D. dendriticum is the common liver flukes in ruminants, in Iran [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Characterization of Dicrocoelium spp. Isolates from Sheep, Goat and Cattle in the West of Iran

et al. 2020

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D ICROCELIOSIS is a prevalent parasitic disease in ruminants and in humans and is accidental caused by different species of Dicrocoelium spp. This study aimed to evaluate the morphological characteristics and molecular parameters of Dicrocoelium spp. isolated from livers of slaughtered sheep, goat, and cattle in three provinces in west of Iran from Feb 2018 to Feb 2019. In the present study, 45 infected livers with Dicrocoelium were collected (each province 15 and each host 5 infected liver). After morphological examination, the molecular evaluation was carried out for the ribosomal DNA (partial 5.8S, complete ITS2 and partial 28S genes) using PCR and PCR-RFLP techniques. Morphological analysis revealed that all specimens belonged to Dicrocoelium dendriticum. There were profound morphometric variations among D. dendriticum from different hosts and sampling areas, but no statistically significant differences was found (P>0.001). The sequencing analysis of obtained fragments in GeneBank showed 96% to 100% similarity within homogeneous fragments in the database. The obtained sequences were also registered in Gene Bank with the accession numbers, MN831473, MN831474 and MN831475. Additionally, all PCR-RFLP products revealed two similar fragments (319bp and 271 bp) in all examined samples. According to the present study, haplotype A was shown to be the predominant haplotype of D. dendriticum in the west of Iran.

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Genetic variability amongDicrocoelium dendriticumisolates from different regions in Shaanxi Province, China revealed by sequences of three mitochondrial genes

Cited by 5 publications

References 29 publications

Validation of deep amplicon sequencing of Dicrocoelium in small ruminants from Northern regions of Pakistan

Validation of deep amplicon sequencing of Dicrocoelium in small ruminants from Northern regions of Pakistan

Rapid Detection and Identification of Fasciola spp. and Dicrocoelium spp. Isolated from the Ruminant Livestock of Northwest Iran Using High-Resolution Melting Analysis (HRM)

Morphological and Molecular Characterization of Dicrocoelium spp. Isolates from Sheep, Goat and Cattle in the West of Iran

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