Genetic variability among coat protein of Prune dwarf virus variants from different countries and different Prunus species

Kalinowska, E.; Mroczkowska, Karolina; Paduch-Cichal, E.; Chodorska, M.

doi:10.1007/s10658-014-0502-x

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…As suggested by Paduch-Cichal et al [15] and Paduch-Cichal and Sala-Rejczak [16], absorbance above 0.2 confirmed the presence of the virus. Significance threshold values of DAS-ELISA were determined according to [6,15,16]. Absorbance from mock-inoculated plants was much lower than this threshold.…”

Section: Virus Inoculation and Das-elisamentioning

confidence: 99%

“…Samsun [3,11,12,24]. Here, PDV multiplies to higher levels, easily detectable by DAS-ELISA [6]. In our research we have started analyzing the PDV test plant quinoa (C. quinoa).…”

Section: Symptoms Of Pdv Infection and Immunofluorescent Localizationmentioning

confidence: 99%

“…Vegetative reproduction is crucial for orchard trees. A decrease during vegetative reproduction can reach up to 90% in nurseries [3,6]. PDV can also be transmitted via seeds and pollen of infected plants [3].…”

Section: Introductionmentioning

confidence: 99%

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Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV)

Kozieł

Otulak

Bujarski

2020

Cells

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Prune dwarf virus (PDV) is a plant RNA viral pathogen in many orchard trees worldwide. Our knowledge about resistance genes or resistant reactions of plant hosts to PDV is scant. To fill in part of this gap, an aim of this study was to investigate reactions to PDV infection in a model host, Chenopodium quinoa. Our investigations concentrated on morphological and ultrastructural changes after inoculation with PDV strain 0599. It turned out that PDV infection can cause deformations in host cells but also induce changes in the organelles, such as chloroplasts in inoculated leaves. Moreover, we also demonstrated specific reactions/changes, which could be associated with both types of vascular tissue capable of effectively blocking the systemic spread of PDV to upper leaves. Furthermore, the relative amount of virus, P1 protein deposition, and movement protein (MP) gene expression consequently decreased in PDV-inoculated leaves.

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Section: Virus Inoculation and Das-elisamentioning

confidence: 99%

“…Samsun [3,11,12,24]. Here, PDV multiplies to higher levels, easily detectable by DAS-ELISA [6]. In our research we have started analyzing the PDV test plant quinoa (C. quinoa).…”

Section: Symptoms Of Pdv Infection and Immunofluorescent Localizationmentioning

confidence: 99%

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Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV)

Kozieł

Otulak

Bujarski

2020

Cells

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“…The genome of PDV, like other Ilarviruses , is multipartite and consists of 3 viral RNA (vRNA) molecules: RNA1, RNA2 and RNA3 [ 1 , 6 ]. RNA1 and RNA2 encode two replication proteins, the “replicase” (P1 protein) and RNA-dependent RNA polymerase (RdRp, P2 protein), respectively [ 7 , 8 ]. Both P1 and P2 are required for replication of vRNAs and build replication complexes during viral infection [ 1 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Analysis of Prune Dwarf Virus Intercellular Transport and Pathogenesis

Kozieł

Otulak

Bujarski

2018

IJMS

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Prune dwarf virus (PDV) is an important viral pathogen of plum, sweet cherry, peach, and many herbaceous test plants. Although PDV has been intensively investigated, mainly in the context of phylogenetic relationship of its genes and proteins, many gaps exist in our knowledge about the mechanism of intercellular transport of this virus. The aim of this work was to investigate alterations in cellular organelles and the cell-to-cell transport of PDV in Cucumis sativus cv. Polan at ultrastructural level. To analyze the role of viral proteins in local transport, double-immunogold assays were applied to localize PDV coat protein (CP) and movement protein (MP). We observe structural changes in chloroplasts, mitochondria, and cellular membranes. We prove that PDV is transported as viral particles via MP-generated tubular structures through plasmodesmata. Moreover, the computer-run 3D modeling reveals structural resemblances between MPs of PDV and of Alfalfa mosaic virus (AMV), implying similarities of transport mechanisms for both viruses.

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“…All three viral RNA components are required for infection of host cells (Bachman et al 1994;Pallas et al 2012;Kalinowska et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Subcelullar localization of proteins associated with Prune dwarf virus replication

et al. 2017

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Prune dwarf virus (PDV) is one of the most dangerous pathogens of fruit trees worldwide. One of the most important proteins required for PDV infection is replicase. (P1 protein) which anchored viral RNA and builds replication complex along with RNA depended polymerase. Despite the importance of PDV as a pathogen, our knowledge regarding tissue/cellular localization and structure of PDV P1 protein is still incomplete. The aim of this work was to localize replicase distribution in leaf tissues and cells by immunofluorescent and immunogold labeling of Nicotiana tobaccum cv Samsun and development of a 3D model of PDV replicase. In this paper we demonstrate that PDV replication, is similar to that of Alfalfa mosaic virus and is strongly connected with tonoplasts. In addition, PDV replicase and coat protein (CP) were also found to be strongly associated with membranes of endoplasmic reticulum and, indicating the potential involvement of these membrane structures in the processes related to viral infection. Bioinformatic analyzes based on 3D modeling and structure prediction revealed that P1 protein has a potential transmembrane domain which enables protein anchoring to tonoplast during replication complex assembly.

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Genetic variability among coat protein of Prune dwarf virus variants from different countries and different Prunus species

Cited by 13 publications

References 20 publications

Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV)

Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV)

Ultrastructural Analysis of Prune Dwarf Virus Intercellular Transport and Pathogenesis

Subcelullar localization of proteins associated with Prune dwarf virus replication

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