Genetic typing of classical swine fever virus

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

“…The confidence levels indicated by statistically significant bootstrap levels for its branch nodes were highest for the NS5B tree (23 values) and lowest for the 5 0 UTR tree (only one value). Similar type of reliable classification was obtained with sequence data from the NS5B region by Paton et al [16].The discrimination possible between isolates depends on the length and variability of the target region of the genome that is used for comparisons [16]. Among all isolates compared, only one pair appears identical in sequences from the E2 region and four pairs from the NS5B region when compared to 31 pairs from the 5 0 UTR (Figs.…”

“…Genetic typing of CSFV isolates and comparison with other Indian isolates and those originating from other countries is important so as to determine the genetic diversity, molecular epidemiology and to establish a control strategy against CSF [16]. In the present study phylogenetic analysis was done for E2, NS5B and 5 0 UTR to obtain the most reliable genetic typing of the CSFV isolates.…”

“…Indian isolates cluster on the phylogenetic tree is encircled. The nomenclature of the Groups and subgroups was as per Paton et al [16] group. The nomenclature of the groups and subgroups was done as per Paton et al [16].…”

“…The nomenclature of the Groups and subgroups was as per Paton et al [16] group. The nomenclature of the groups and subgroups was done as per Paton et al [16]. Aligned sequences of all the three regions are available with authors.…”

“…A compilation of data from OIE indicates that there were 1308 outbreaks of CSF in India during 1996-2008 [16]. It is one of the top five viral diseases of livestock in the country according to data available in NIVEDI [15].…”