Genetic typing of bovine viral diarrhoea virus: most Slovenian isolates are of genotypes 1d and 1f

Toplak, Ivan; Sandvik, T.; Barlič–Maganja, Darja; Grom, Jože; Paton, David

doi:10.1016/j.vetmic.2003.12.004

Cited by 54 publications

(35 citation statements)

References 32 publications

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“…7,15,17 This procedure requires a previous extraction step to obtain pure nucleic acids free of inhibitors. Two different extraction methods were used in the present study, phenol-chloroform for total nucleic acids extraction for the naturally infected samples and a commercial kit b for viral RNA for the experimentally infected samples.…”

Section: Discussionmentioning

confidence: 99%

Detection ofBorder Disease Virusin Fetuses, Stillbirths, and Newborn Lambs from Natural and Experimental Infections

et al. 2009

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Abstract. The purpose of the present study was to evaluate the use of enzyme-linked immunosorbent assay (ELISA) antigen detection in blood or fetal fluids and reverse transcription polymerase chain reaction (RT-PCR) amplification in tissues for routine laboratory diagnosis of Border disease virus (BDV) infection. Samples from 67 fetuses, 6 stillbirths, and 11 lambs from 25 commercial flocks with suspicion of BDV abortion and 3 fetuses, 7 stillbirths, and 15 lambs obtained from an experimental infection with a local isolate (BDV genotype 4) were investigated. Presence of BDV was detected by RT-PCR in 7.9% of fetuses, 50% of stillbirths, and 50% of lambs from the commercial flocks analyzed, corresponding to 8 of the 25 farms (32%). A similar percentage of the lambs and stillbirths from the experimental infection were positive by RT-PCR of tissue samples (54.5%), and the highest positivity was detected in lymph node, thyroid gland, and kidney. The current study revealed that RT-PCR analysis of stillbirths and lambs with clinical symptoms is more suitable than the analysis of fetuses to confirm the presence of BDV in a flock. Pestiviral antigen was detected by antigen ELISA in a high proportion of fetuses (24/58) and stillbirths (3/4) from commercial flocks, but in lambs, the presence of colostral antibodies masked the detection of the antigen by ELISA. Nevertheless, in lambs from the experimental infection that were not fed colostrum, antigen ELISA was less efficient than RT-PCR in detecting viral presence in stillbirths and lambs. Antigen ELISA is therefore recommended for fetuses with advanced autolysis that can adversely affect RNA integrity.

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Section: Discussionmentioning

confidence: 99%

Detection ofBorder Disease Virusin Fetuses, Stillbirths, and Newborn Lambs from Natural and Experimental Infections

et al. 2009

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“…One-step RT-PCR was performed using a Transcriptor One-Step RT-PCR Kit (Roche Diagnostics, Germany) according to the manufacturer's instructions using panpestivirus primers (20), which amplify a fragment of viral 5'UTR region and primers designed for amplification of partial N pro coding region (19). The reaction conditions were set at 50°C for 30 min and 94°C for 7 min, followed by 10 cycles at 94°C for 10 s, 53°C for 30 s, 68°C for 30 s, and then 25 cycles at 94°C for 10 s, 53°C for 30 s, 68°C for 33 s with a final extension step at 68°C for 7 min.…”

Section: Animalsmentioning

confidence: 99%

Viral shedders in a herd vaccinated against infection with bovine viral diarrhoea virus (BVDV) without prior testing for the presence of persistently infected animals

Polak

Antos

Rola

et al. 2016

Journal of Veterinary Research

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Introduction: Bovine viral diarrhoea (BVD), caused by the bovine viral diarrhoea virus (BVDV), is one of the most important diseases of cattle worldwide. The purpose of the study was to determine the BVDV infection status in a dairy herd vaccinated against BVD. Before vaccination started in 2008, there had been no prior identification or the removal of the possible source of infection (persistently infected animals). It was expected that vaccination itself would enable the elimination of viral shedders on a long term basis. Material and Methods: Serological screening for antibodies against BVDV with determination for antibodies titres, BVDV antigen, and the presence of the viral genome with phylogenetic analysis of positive samples in the herd were performed, despite the lack of any clinical problems indicating possible presence of BVDV infection. Results: 19 individuals persistently infected with BVDV were identified among calves and heifers but not in adult cattle. All virus shedders were antibody negative and the genotype of isolated virus was BVDV-1b, indicating a single source of infection. The vaccine used in the herd was composed of BVDV-1a strain. In each of the tested cowsheds, antibody titres against BVDV-1b were higher than against BVDV-1a (median values). Conclusion: Despite a long-lasting vaccination programme and relatively high sequence homology of vaccinal and field strains of BVDV (83.6%), it was not possible to avoid transplacental infections of foetuses and the birth of persistently infected calves from vaccinated heifers although the protection against clinical disease was accomplished.

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“…The genetic typing of BVDV has most frequently been based on sequence analysis of the 5' NCR, Npro or E2 regions (Vilcek, et al, 2001;Becher, et al, 2003;Nagai et al, 2004;Toplak et al, 2004). Analysis of the 5' NCR, a highly conserved region of the genome, has shown to be a reliable and reproducible method for genetic characterization of BVDV isolates (Ridpath, 2005b).…”

Section: Genotypingmentioning

confidence: 99%

Diagnostic Approaches for Detection of Bovine Viral Diarrhea Virus Persistent Infection

Ahmad¹

2014

J. Inf. Mol. Biol.

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Bovine viral diarrhea is one of the most important diseases of cattle which is causing continuous economic losses to the cattle industry primarily due to decreased reproductive performance. Without doubt, direct contact between BVDV persistently infected, and susceptible animals is the most important transmission route of virus. All control programs which are in use in many countries of the world, mainly depend upon the detection of PI animals, eliminating them and preventing their return into the herds. Various diagnostic tests using ear notch biopsies and serum samples are in use with certain advantages and disadvantages. In detecting the BVDV persistent infection, a complete agreement (P value = 1) was observed between Real time RT-PCR, AC-ELISA, VI and IHC. All four assays were found specific but real time RT-PCR was found to be more sensitive. Both, VI and IHC were found labour intensive, as diagnosis may take more than one week to be made. Further peroxidase based IHC interpretation was found to be difficult somewhat due to subjective reasoning. It is generally recognized, that real time RT-PCR is more sensitive for BVDV detection than the other methods employed in the study period. However, use of real time RT-PCR for screening of individual animal cases is cost prohibited. AC-ELISA while relatively less sensitive was shown to has sufficient sensitivity for cost effective identification of PI. The results appear to warrant the use of AC-ELISA on ear notch biopsies for routine diagnosis of PI animals followed by real time RT-PCR on suspicious samples.All copyrights reserved to Nexus® academic publishers

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Genetic typing of bovine viral diarrhoea virus: most Slovenian isolates are of genotypes 1d and 1f

Cited by 54 publications

References 32 publications

Detection ofBorder Disease Virusin Fetuses, Stillbirths, and Newborn Lambs from Natural and Experimental Infections

Detection ofBorder Disease Virusin Fetuses, Stillbirths, and Newborn Lambs from Natural and Experimental Infections

Viral shedders in a herd vaccinated against infection with bovine viral diarrhoea virus (BVDV) without prior testing for the presence of persistently infected animals

Diagnostic Approaches for Detection of Bovine Viral Diarrhea Virus Persistent Infection

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