Genetic Types, Gene Repertoire, and Evolution of Isolates of the Salmonella enterica Serovar 4,5,12:i:− Spanish Clone Assigned to Different Phage Types

Garcı́a, Patricia; Malorny, Burkhard; Hauser, Elisabeth; Mendoza, M. C.; Rodicio, M. Rosario

doi:10.1128/jcm.02777-12

Cited by 26 publications

(27 citation statements)

References 30 publications

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“…4,[5],12:i:- using the same MLVA scheme, also observed that the discriminatory power was exclusively related to the diversity of STTR5 and 6. Other authors ( Hopkins et al, 2010 , 2012 ; Gallati et al, 2013 ; Garcia et al, 2013 ; Arguello et al, 2014 ; Boland et al, 2014 ), who characterized epidemiologically unrelated isolates of S. 4,[5],12:i:- by MLVA, reported profiles similar to those found in the present study. Since MLVA is a highly discriminatory method, Hopkins et al (2007) reported that some minor changes in targeted loci could be tolerated among related outbreak isolates.…”

Section: Discussionsupporting

confidence: 86%

Ascertaining the relationship between Salmonella Typhimurium and Salmonella 4,[5],12:i:- by MLVA and inferring the sources of human salmonellosis due to the two serovars in Italy

et al. 2015

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The current picture of human salmonellosis shows Salmonella Typhimurium and S. 4,[5],12:i:- as the most common serovars in Italy. The aims of this study were to investigate the genetic relationship between these serovars, as well as to test the possibility of inferring sources of human salmonellosis due to S. Typhimurium and S. 4,[5],12:i:- by using multilocus variable-number tandem repeat analysis (MLVA) subtyping data. Single isolates from 268 human sporadic cases and 325 veterinary isolates (from pig, cattle, chicken, and turkey) collected over the period 2009–2011 were typed by MLVA, and the similarities of MLVA profiles were investigated using different analytical approaches. Results showed that isolates of S. 4,[5],12:i:- were more clonal compared to S. Typhimurium and that clones of both serovars from different non-human sources were very close to those which were responsible for human infections, suggesting that source attribution by MLVA typing should be possible. However, using the Asymmetric Island Model it was not possible to obtain a confident ranking of sources responsible for human infections based on MLVA profiles. The source assignments provided by the model could have been jeopardized by the high heterogeneity found within each source and the negligible divergence between sources as well as by the limited source data available, especially for some species.

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Section: Discussionsupporting

confidence: 86%

Ascertaining the relationship between Salmonella Typhimurium and Salmonella 4,[5],12:i:- by MLVA and inferring the sources of human salmonellosis due to the two serovars in Italy

et al. 2015

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“…The isolates belonging to the Spanish clone presented phenotypic and genotypic markers previously described [10,29]. This clonal lineage, originally identified in Spain where it still predominates [10,29], was subsequently reported in food products, environment or piggeries and is causing human infections in neighboring Portugal since 2002 [8,19]. Intense commercial trade between Portugal and Spain might explain the maintenance and dissemination of the Spanish clone in the Iberian Peninsula.…”

Section: Discussionmentioning

confidence: 91%

“…Furthermore, we found several patterns of genomic deletions surrounding the fljAB region, including those previously described for European clone isolates [11] or in atypical US strains (32 % of our isolates) [13], suggesting that multiple independent emergence events occurred in ST34 clonal lineage, most likely from S. Typhimurium. The isolates belonging to the Spanish clone presented phenotypic and genotypic markers previously described [10,29]. This clonal lineage, originally identified in Spain where it still predominates [10,29], was subsequently reported in food products, environment or piggeries and is causing human infections in neighboring Portugal since 2002 [8,19].…”

Section: Discussionmentioning

confidence: 94%

Characterization of the emerging clinically-relevant multidrug-resistant Salmonella enterica serotype 4,[5],12:i:- (monophasic variant of S. Typhimurium) clones

Mourão

Machado

Novais

et al. 2014

Eur J Clin Microbiol Infect Dis

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To better understand the recent success/emergence of Salmonella enterica serotype 4,[5],12:i:- we characterized the population diversity, fljAB deletion patterns, antibiotic resistance features and associated genetic elements of a comprehensive collection obtained in the last decade from Portugal (2002-2010). One hundred thirty-one isolates from human clinical specimens, food, environment and piggeries, verified by PCR as S. 4,[5],12:i:-, were studied for clonality (Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing), antibiotic resistance by phenotypic (disk diffusion and/or agar dilution) and genotypic (PCR/Restriction Fragment Length Polymorphism and sequencing, genomic location) methods and fljAB-deletions (PCR). Plasmid analysis included determination of size, content and characterization of the incompatibility group (PCR-Based Replicon Typing and I-CeuI/S1-hybridization). Results showed three multidrug-resistant (MDR) clones circulating and causing infections, associated with particular phenotypic and genotypic features. Most of the isolates belonged to the widespread European (ASSuT phenotype, RR1-RR2 resistance regions, ST34) and Spanish (carrying a sul3-type III integron within IncA/C plasmids, ST19) clones circulating in Europe. A third clone, here designated Southern European clone (carrying a sul3-type I integron within IncR plasmids, ST19), presents a fljAB region different from the previous clones and similar to the US strains, despite differences in the MDR mobile genetic platforms. The success of S. 4,[5],12:i:- might be related to the selective advantage offered by MDR profiles associated with stable genetic elements, also carrying virulence features, along with well adapted clones to the animal food production and causing human infections.

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“…MLVA patterns found among the 59 S. 4, [5],12:i:-isolates did not match with the major MLVA patterns observed in the recent study on the Spanish clone (García et al, 2013), but three of the most prevalent MLVA patterns in this study, i.e. 3-13-11-NA-211, 3-13-10-NA-211, and 3-12-10-NA-211, were commonly found in other European countries (Hopkins et al, 2010;Argüello et al, 2014).…”

Section: Discussionmentioning

confidence: 80%

Characterization of Salmonella enterica serovars Tyhimurium and 4,5,[12]:i:- isolated from pigs and pig environmental-related sources in the NE of Spain

Andrés-Barranco

Vico

Marín

et al. 2015

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

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A total of 117 Salmonella Typhimurium and 59 monophasic Salmonella Typhimurium (S. 4, [5],12:i:-) strains isolated from pig, wild bird, rodent, and farm environmental samples were characterized by phage typing, antibiotic susceptibility testing and Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) in order to evaluate their phenotypic and genetic relatedness. In S. 4, [5],12:i:-the most prevalent phage types were U311 (40.7%) and DT195 (22%) which did not correspond with the so-called Spanish clone, and generally showed a different resistant pattern (ASSuT). Antibiotic resistance was found in 85.8% of the isolates, with 94.1% of them displaying multidrug resistance (MDR). MLVA identified 92 different profiles, six of them shared by both serovars. The Minimum Spanning Tree showed one major cluster that included 95% of the S.

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Genetic Types, Gene Repertoire, and Evolution of Isolates of the Salmonella enterica Serovar 4,5,12:i:− Spanish Clone Assigned to Different Phage Types

Cited by 26 publications

References 30 publications

Ascertaining the relationship between Salmonella Typhimurium and Salmonella 4,[5],12:i:- by MLVA and inferring the sources of human salmonellosis due to the two serovars in Italy

Ascertaining the relationship between Salmonella Typhimurium and Salmonella 4,[5],12:i:- by MLVA and inferring the sources of human salmonellosis due to the two serovars in Italy

Characterization of the emerging clinically-relevant multidrug-resistant Salmonella enterica serotype 4,[5],12:i:- (monophasic variant of S. Typhimurium) clones

Characterization of Salmonella enterica serovars Tyhimurium and 4,5,[12]:i:- isolated from pigs and pig environmental-related sources in the NE of Spain

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