Genetic translocation in Staphylococcus aureus.

Novick, Richard P.; Edelman, Isidore S.; Schwesinger, M D; Gruss, Alexandra; Swanson, E C; Pattee, P A

doi:10.1073/pnas.76.1.400

Cited by 118 publications

(72 citation statements)

References 12 publications

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“…This 5.2 kb transposon specifies resistance to Em and other macrolide antibiotics in S. aureus; it can transpose to many different sites in the S . aweus chromosome and in plasmids (Novick et al, 1979a;Pattee, 1981). Plasmid pRN3032, a temperature-sensitive Cdr plasmid that carries Tn551, was transferred to strains A70 and A53 by transduction from ISP479, selecting for Emr and Cdr.…”

Section: Gene Transfer Experimentsmentioning

confidence: 99%

Distinct groups of plasmids correlated with bacteriocin production in Staphylococcus aureus

Giambiagi-Marval¹,

Mafra²,

Penido³

et al. 1990

Journal of General Microbiology

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The genetic basis of bacteriocin (Bac) production by six strains of Stuphylococcus aureus was examined. Gene transfer experiments (in which the plasmids were tagged with the erythromycin resistance transposon Tn551) and plasmid-elimination experiments by growth at 43 "C associated bacteriocin production with a particular plasmid in each strain. The Bac plasmids could be separated into two distinct groups: the first comprised plasmids larger than 40 kb, which did not specify immunity to bacteriocins; the second comprised small plasmids (8.0-10-4 kb) which also specified immunity to bacteriocins. The sequence relations among the small plasmids (pRJ6, pRJ9, pRJlO and pRJ11) were investigated by comparing restriction enzyme digest patterns and by hybridization. Plasmids pRJlO and pRJ11 were indistinguishable and very closely related to plasmid pRJ9. Plasmid pRJ6, although different from the others, shared regions of sequence homology with them. No homology was found between plasmids pRJ6 or pRJ9 and the large Bac plasmids.

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Section: Gene Transfer Experimentsmentioning

confidence: 99%

Distinct groups of plasmids correlated with bacteriocin production in Staphylococcus aureus

Giambiagi-Marval¹,

Mafra²,

Penido³

et al. 1990

Journal of General Microbiology

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“…Chromosomal insertions of TnS51 (a 5.2-kilobase transposon that confers resistance to erythromycin [7,10]) are identified by the symbol fl, followed by an isolation number (13). pCRG1690 is a gentamicin-resistant, self-transmissible plasmid that has been described by Asch et al (1).…”

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confidence: 99%

Chromosomal gene transfer during conjugation by Staphylococcus aureus is mediated by transposon-facilitated mobilization

Stout

Iandolo

1990

J Bacteriol

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A chromosomal copy of the transposon TnS51 and a copy coresident on a gentamicin-resistant conjugative plasmid of Staphylococcus aureus resulted in the mobilization of chromosomal genes during filter mating. Gene mobilization was recA dependent and was not restricted to any specific region of the chromosome. Both essential and nonessential genes were transferred.The use of conjugative plasmids to mobilize chromosomal genes in gram-negative bacteria is well known (5,6,8,15). Although insertion sequences mediate mobilization, transposons have also been used as portable regions of homology to promote integration of the plasmids into the chromosome. The same transposon is inserted into both the plasmid and the chromosome of a single donor cell for recombination or insertion or both. Using a conjugative plasmid and a transposon from Staphylococcus aureus, we have also achieved chromosome mobilization with a tactic similar to those described for use with gram-negative bacteria. This study reports the construction and utilization of donor cells for the mobilization of genes on either side of the chromosomal insertion.Except for ISP187, the strains of S. aureus used were isogenic derivatives of strain NCTC 8325. Chromosomal insertions of TnS51 (a 5.2-kilobase transposon that confers resistance to erythromycin [7,10]) are identified by the symbol fl, followed by an isolation number (13). pCRG1690 is a gentamicin-resistant, self-transmissible plasmid that has been described by Asch et al. (1). pCRG1690::fl(TnS51)3 is a derivative of pCRG1690 which contains a copy of TnS51 and was provided by P. Pattee (Iowa State University).Brain heart infusion (BHI) medium was used to culture the organisms. Complete defined synthetic agar (12) was used as a base when screening for fusidic acid-resistant and prototrophic transconjugants. Individual amino acids and purines were omitted, or fusidic acid (10 p.g/ml) was added.Selection and screening for other antibiotic resistance markers were performed by using BHI agar with the appropriate antibiotic added at the following concentrations: 5 ,ug of penicillin G per ml, 3 jxg of tetracycline per ml, 10 ,ug of erythromycin per ml, 30 ,ug of novobiocin per ml, 10 jig of gentamicin per ml, and 30 ,ug of chloramphenicol per ml.Nitrocellulose membrane filter matings were conducted by the procedure of McDonnell et al. (9) with the following modifications. Cultures of donor and recipient cells were grown overnight at 37°C with shaking in 10 ml of BHI in tubes at 100 rpm. The cultures were diluted 1:20 in fresh BHI, and 1 ml of each was filtered through a sterile 0. 45-,um-

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“…The occurrence of apparently nonhomologous recombinational events in S. aureus has been observed repeatedly, especially in the case of antibiotic resistance determinants. Such events, involving, among other things, insertion of plasmid fragments into the chromosome, transfer of DNA sequences from the chromosome into plasmids (19,22,24), or the stable integration of entire plasmids into the chromosome (26) can be most easily interpreted by translocatable elements or insertion sequences. Indeed, a clear-cut example of genetic translocation in S. aureus involving the 5.2-kilobase transposon Tn551 has recently been described (24).…”

Section: Discussionmentioning

confidence: 99%

“…Such events, involving, among other things, insertion of plasmid fragments into the chromosome, transfer of DNA sequences from the chromosome into plasmids (19,22,24), or the stable integration of entire plasmids into the chromosome (26) can be most easily interpreted by translocatable elements or insertion sequences. Indeed, a clear-cut example of genetic translocation in S. aureus involving the 5.2-kilobase transposon Tn551 has recently been described (24). It could be that illegitimate recombination of genes, plasmids, or plasmid fragments, coding for resistance to aminocycitols, has also occurred in the S. aureus strains FK170 and 5532.…”

Section: Discussionmentioning

confidence: 99%

Aminocyclitol-modifying enzymes specified by chromosomal genes in Staphylococcus aureus

Kayser¹,

Homberger²,

Devaud³

1981

Antimicrob Agents Chemother

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A genetic analysis of aminocyclitol resistance in two strains of Staphylococcus aureus was performed. Resistance of strain FK170, isolated in Zurich, was due to the production of a 3'-phosphotransferase [APH(3')]. Strain 5532, isolated in London, produced a 2"-phosphotransferase [APH(2")] and a 6'-N-acetyltransferase [AAC(6')]. Plasmid deoxyribonucleic acid (DNA) was isolated by isopycnic centrifugation from the two parent strains, as well as from susceptible variants and from resistant transductants of both strains. Comparative analysis of plasmid and from resistant transductants of both strains. Comparative analysis of plasmid DNA by centrifugation in sucrose gradients revealed that strain FK170 harbored a 2.7-megadalton tetracycline R-plasmid and a 36-megadalton cryptic plasmid. Strain 5532 contained an 18.5-megadalton penicillinase plasmid. No evidence for plasmid location of the markers for aminocyclitol resistance could be obtained.

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Genetic translocation in Staphylococcus aureus.

Cited by 118 publications

References 12 publications

Distinct groups of plasmids correlated with bacteriocin production in Staphylococcus aureus

Distinct groups of plasmids correlated with bacteriocin production in Staphylococcus aureus

Chromosomal gene transfer during conjugation by Staphylococcus aureus is mediated by transposon-facilitated mobilization

Aminocyclitol-modifying enzymes specified by chromosomal genes in Staphylococcus aureus

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