Genetic transformation of Vitis viniferavia organogenesis

Mezzetti, Bruno; Pandolfini, Tiziana; Navacchi, O.; Landi, L.

doi:10.1186/1472-6750-2-18

Cited by 73 publications

(33 citation statements)

References 24 publications

(24 reference statements)

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“…Genetic transformation via adventitious shoot regeneration has been achieved in numerous dicotyledonous plant species, such as soybean (Wang and Xu 2008), mulberry (Agarwal et al 2004;Agarwal and Kanwar 2007), pomegranate (Terakami et al 2007), tomato (Micro-Tom) (Sun et al 2006), strawberry (Mezzetti and Costantini 2006), cherry Sink 2005, 2006), carnation (Nontaswatsri and Fukai 2006), Antirrhinum (Cui et al 2004), eucalyptus (Tournier et al 2003), sweet orange (Almeida et al 2003), grape (Mezzetti et al 2002), and peanut (Sharma and Anjaiah 2000;Sharma and Bhatnagar-Mathur 2006). In most cases, cotyledons or hypocotyls from in vitro grown seedlings are used as the target tissues for Agrobacterium infection (Agarwal et al 2004;Cui et al 2004;Sun et al 2006;Agarwal and Kanwar 2007;Wang and Xu 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In most cases, cotyledons or hypocotyls from in vitro grown seedlings are used as the target tissues for Agrobacterium infection (Agarwal et al 2004;Cui et al 2004;Sun et al 2006;Agarwal and Kanwar 2007;Wang and Xu 2008). However, transgenic plants are also efficiently regenerated from explants of leaves (Tournier et al 2003;Sink 2005, 2006;Mezzetti and Costantini 2006;Terakami et al 2007), nodes (Nontaswatsri and Fukai 2006), and shoot apices (Mezzetti et al 2002) of in vitro grown plants via adventitious organogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Production of transgenic pineapple (Ananas cosmos (L.) Merr.) plants via adventitious bud regeneration

Wang

Uruu

Xiong

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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A new protocol for the production of transgenic pineapple plants was developed. Adventitious buds were induced directly from Agrobacterium-infected leaf bases and stem discs of in vitro plants, bypassing the establishment of callus cultures. Non-chimeric transgenic plants were obtained by multiple subculturing of primary transformants under increasing levels of selection. A total of 42 independent transgenic lines were produced from two cultivars with two different constructs: one containing a modified rice cystatin gene (Oc-IΔD86) and the other with the anti-sense gene to pineapple aminocyclopropane synthase (ACS). GUS histochemical staining provided the first evidence of the non-chimeric nature of the transformed plants. Their non-chimeric nature was further demonstrated by PCR analyses of the DNA extracted from individual leaves of a primary transformed plant and also from multiple plants propagated from a single transformation event. Southern hybridization confirmed random integration patterns of transgenes in the independent lines. For the Oc-IΔD86 gene, the expression at the mRNA level was detected via RT-PCR and its translation was detected by protein blot. Agronomic evaluation and bioassays of the transgenic plants will further validate the utility of this new tool for pineapple improvement.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production of transgenic pineapple (Ananas cosmos (L.) Merr.) plants via adventitious bud regeneration

Wang

Uruu

Xiong

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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“…The in vitro regeneration protocol based on the production of meristematic bulks [12] was efficiently optimized for different grapevine cultivars (Glera, Sangiovese, Thompson Seedless, Vermentino) and rootstocks (1103 Paulsen, 110 Richter) (Fig. 2).…”

Section: Induction Of Mbs From Different Grapevine Cultivars and Rootmentioning

confidence: 99%

“…In vitro grapevine regeneration has been achieved principally by somatic embryogenesis using different tissues as starting explants, such as anthers, leaves and ovaries [6][7][8]. In vitro grapevine shoots regeneration was also obtained via organogenesis, which represents a good alternative regeneration method to somatic embryogenesis, avoiding some technical problems related to embryogenetic cultures induction and maintenance [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…In the present study the regeneration protocol, via organogenesis, based on the induction of meristematic bulks [12], was optimized for different grapevine cultivars (Glera, Vermentino, Sangiovese, Thompson Seedless) and rootstocks (1103 Paulsen, and 110 Richter). To investigate the transformation efficiency of the different genotypes, the meristematic bulks created for each clone were used at the beginning as explants for Agrobacteriummediated genetic transformation with a gene construct that expresses the enhanced green fluorescent protein (e-GFP) and neomycin phosphotransferase (nptii) as marker genes.…”

Section: Introductionmentioning

confidence: 99%

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A plant regeneration platform to apply new breeding techniques for improving disease resistance in grapevine rootstocks and cultivars

et al. 2019

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Worldwide grapevine cultivation is based on the use of elite cultivars, in many cases strictly linked to local important wine brands. Most of Vitis viniferacultivars have high susceptibility to fungal and viral diseases therefore, new breeding techniques (e.g. Cisgenesis, RNAi and gene editing) offer the possibility to introduce new clones of the main cultivars with increased diseases resistance, in order to reduce environmental impact and improve quality in the intensive wine grape industry. This study is finalized to develop efficient in vitro regeneration and transformation protocols to extend the application of these technologies in wine grape cultivars and rootstocks. With this aim, in vitro regeneration protocols based on the production of meristematic bulks (Mezzetti et al., 2002) were optimized for different grapevine cultivars (Glera, Vermentino, Sangiovese, Thompson Seedless) and rootstocks (1103 Paulsen, and 110 Richter). The meristematic bulks were then used as explants for Agrobacteriummediated genetic transformation protocols, by comparing the use of NPTII and e-GFP as marker genes. Results confirmed the efficiency of meristematic bulks as the regenerating tissue to produce new modified plants in almost all the above genotypes. The highest regeneration efficiency in some genotypes allowed the selection of stable modified lines/calli with only the use of e-GFP marker gene. This protocol can be applied in the use of MYB marker gene for the production of cisgenic lines. Genotypes having the highest regeneration and transformation efficiency were also used for transformation experiments using a hairpin gene construct designed to silence the RNA-dependent RNA polymerase (RpRd) of the GFLV and GLRaV3, which would induce multiple virus resistances, and the Dicer-like protein 1 (Bc-DCL1) and Bc-DCL2 to control B. cinerea infection.

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Grapes

Bouquet

Torregrosa

Iocco

et al. 2008

Compendium of Transgenic Crop Plants

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Grapevine ( Vitis vinifera ) is one of the major fruit crops in the world based on hectares cultivated and economic value, essentially due to the production of wine. The premium quality wines are produced from cultivars that enjoy a high level of consumer acceptance and are firmly entrenched in the market place. Transgenic breeding is attractive when compared to conventional breeding, because it offers a means of inserting new characters, such as disease resistance, into the genome of traditional cultivars without changing any of their other characteristics particularly wine typicity and quality. The aim of this review is to provide an outlook on the usefulness of genetic transformation in grapevine, not only to develop genetically modified cultivars for commercial release, but also as a valuable tool to understand the genetics and mechanisms of plant growth, development, and metabolism. Thus, the availability of improved and efficient techniques of transformation is needed to sustain the current development of grapevine genomics. Even if the future development of transgenic cultivars will depend essentially on a general acceptance of GM plant products by the consumers, it will require a complete and reliable assessment of their environmental and health safety, comparing the potential risks to the expected benefits of their use.

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Genetic transformation of Vitis viniferavia organogenesis

Cited by 73 publications

References 24 publications

Production of transgenic pineapple (Ananas cosmos (L.) Merr.) plants via adventitious bud regeneration

Production of transgenic pineapple (Ananas cosmos (L.) Merr.) plants via adventitious bud regeneration

A plant regeneration platform to apply new breeding techniques for improving disease resistance in grapevine rootstocks and cultivars

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