Genetic transformation of prickly-pear cactus (Opuntia ficus-indica) by Agrobacterium tumefaciens

Silos-Espino, Héctor; Valdéz-Ortiz, Ángel; Rascón-Cruz, Quintín; Rodríguez-Salazar, E.; Paredes‐López, Octavio

doi:10.1007/s11240-006-9123-1

Cited by 22 publications

(17 citation statements)

References 21 publications

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“…However, as with other applications of biotechnology, there has been little progress with cactus. In relation to regeneration of entire transgenic plants, Silos-Espino et al (2006) developed a protocol for the genetic transformation of an elite prickly pear cactus (Opuntia ficus-indica L., cultivar Villa Nueva) by infecting it with Agrobacterium tumefaciens with the aim of using this technology to improve certain agronomic characteristics of the cultivated genotypes of this species. The transgenic character of the regenerated plants was demonstrated by both Polymerase Chain Reaction (PCR) and Southern blot analysis; transgene copy number in the transformed plants ranged from two to six, whereas the transformation frequency was 3 %.…”

Section: Genetic Transformationmentioning

confidence: 99%

Tissue culture of ornamental cacti

Pérez-Molphe-Balch

Santos-Díaz

Ramírez-Malagón

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family.

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Section: Genetic Transformationmentioning

confidence: 99%

Tissue culture of ornamental cacti

Pérez-Molphe-Balch

Santos-Díaz

Ramírez-Malagón

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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“…Somatic embryogenesis-based plant regeneration has also been reported in several cactus species (Santacruz-Ruvalcaba et al, 1998). Callus cultures were used as target tissues to produce transgenic plants in economically important cacti (Al-Ramamneh et al, 2006;Silos-Espino et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Studies on in Vitro Propagation Methods in Cactus Species of the Genera Melocactus, Cereus and Lobivia

Monostori¹,

Tanács²,

Mile³

2012

Acta Hortic.

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In vitro propagation methods are frequently used for the conservation of rare cacti and also for the production of plants for commercial purposes. Here we report on the micropropagation by axillary shoot proliferation at the cactus species Melocactus salvadorensis, Lobivia tegeleriana and Cereus jamacaru being important as ornamentals and also as a forage and fruit crop in the case of the latter one.

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“…A system for genetic transformation of an elite prickly pear cactus (O. ficus-indica L., cultivar Villa Nueva) by Agrobacterium tumefaciens was developed by Silos-Espino et al (2006). Direct bacterial infection of areole meristematic tissue was accomplished with a hypodermic syringe.…”

Section: Genetic Engineering and Molecular Biology Research On Cactusmentioning

confidence: 99%

“…The transformation frequency obtained was 3.2%. The method described by Silos-Espino et al (2006) may be useful for routine transformation and introduction of agronomically important genes in prickly pear cactus.…”

Section: Genetic Engineering and Molecular Biology Research On Cactusmentioning

confidence: 99%

Opuntia and Other Cacti: Applications and Biotechnological Insights

Shedbalkar

Adki

Jadhav

et al. 2010

Tropical Plant Biol.

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The cactus family is unusual among tropical plants. Cacti, known for their minimum water requirement, have been grown extensively in arid lands, for food, feeds and medicinal and therapeutic uses.Several food products have cacti as a main ingredient. Cacti biochemical analysis substantiate the high nutritive value of this plant family. Tissue cultures, including micropropagation, callus, and cell suspension cultures have been established for numerous cacti species. Genetic engineering has opened opportunities for gene isolation and integration of genes from other sources for cacti improvement. Cacti might be a store house of stress tolerant genes for other crops. Since cacti can be cultivated easily with minimum agriculture inputs, they hold great potential for cultivation and farming on degraded lands and for at least partial remediation of degraded lands. The present review outlines some of the older and more recent research on the properties and applications for Opuntia and other cacti especially as they might apply towards agricultural sustainability.

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Genetic transformation of prickly-pear cactus (Opuntia ficus-indica) by Agrobacterium tumefaciens

Cited by 22 publications

References 21 publications

Tissue culture of ornamental cacti

Tissue culture of ornamental cacti

Studies on in Vitro Propagation Methods in Cactus Species of the Genera Melocactus, Cereus and Lobivia

Opuntia and Other Cacti: Applications and Biotechnological Insights

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