Genetic Transformation of Peach Tissues by Particle Bombardment

Ye, Xiaojian; Brown, Susan K.; Scorza, R.; Cordts, John; Sanford, Jay P.

doi:10.21273/jashs.119.2.367

Cited by 52 publications

(19 citation statements)

References 41 publications

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“…Development of genotype-independent transformation procedures will be very difficult to achieve with current technology. Different authors have tried unsuccessfully to transform meristems from micropropagated shoot tips (Ye et al, 1994;Druart et al, 1998). Meristem transformation may eliminate the need for regeneration in production of transgenic plants, allowing genetic manipulation of established cultivars.…”

Section: Shortcomings In the Transformation Of Fruit Treesmentioning

confidence: 99%

Transformation of fruit trees. Useful breeding tool or continued future prospect?

2005

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Regeneration and transformation systems using mature plant material of woody fruit species have to be achieved as a necessary requirement for the introduction of useful genes into specific cultivars and the rapid evaluation of resulting horticultural traits. Although the commercial production of transgenic annual crops is a reality, commercial genetically-engineered fruit trees are still far from common. In most woody fruit species, transformation and regeneration of commercial cultivars are not routine, generally being limited to a few genotypes or to seedlings. The future of genetic transformation as a tool for the breeding of fruit trees requires the development of genotype-independent procedures, based on the transformation of meristematic cells with high regeneration potential and/or the use of regeneration-promoting genes. The public concern with the introduction of antibiotic resistance into food and the restrictions due to new European laws that do not allow deliberate release of plants transformed with antibiotic-resistance genes highlight the development of methods that avoid the use of antibiotic-dependent selection or allow elimination of marker genesfrom the transformed plant as a research priority in coming years.

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Section: Shortcomings In the Transformation Of Fruit Treesmentioning

confidence: 99%

Transformation of fruit trees. Useful breeding tool or continued future prospect?

2005

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“…In this species shoots have been regenerated with high efficiency from in vitro propagated leaves , mature cotyledon (Pooler and Scorza, 1995), immature cotyledon (Mante et al, 1989) and immature zygotic embryos (Hammerschlag et al, 1985). High efficiency of peach bombardment with desired transgenes is also elaborated (Ye et al, 1994), but there are no transformed plants yet. Similar situation, despite of three elaborated regeneration systems, was described by Ainsley et al (2001) for paper shell almond.…”

Section: Agrobacterium Mediated Transformationmentioning

confidence: 99%

Conditions of transformation and regeneration of `Induka' and `Elista' strawberry plants

Gruchała

Korbin

Žurawicz

2004

Plant Cell, Tissue and Organ Culture

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Efficiency of plants' transformation depends on many factors. The genotype, applied techniques and conditions of plant's modification and modified plant regeneration are the most important among them. In our studies regeneration and transformation conditions for two strawberry cultivars were determined and compared. Plants were transformed by Agrobacterium tumefaciens LBA 4404 strain containing plasmid pBIN19 with nptII and gus-reporter genes. Experiment was carried out on more than 1300 leaf explants from each cultivar. Generally, 'Induka' plants characterized with higher regeneration potential than 'Elista'. The highest number of regenerated shoots was obtained on MS medium with 0.4 mg l À1 IBA and 1.8 mg l À1 BA (3.5 and 1.8 shoots/explant for 'Induka' and 'Elista', respectively). After plant transformation number of regenerated, transgenic shoots was higher for 'Elista' (on the average: 8.3 shoots/100 explants). The number of transgenic 'Induka' shoots, obtained at the same conditions, was twice lower (~4.2). Simultaneously 'Induka' plants needed higher kanamycin concentration for transgenic explants selection than 'Elista' (25 mg l À1 ). Preliminary incubation of A. tumefaciens in LB or MS medium with acetosyringone and IAA resulted in increasing transgenic shoots number (per 100 explants: 'Induka' 4.5, 'Elista' 8.0-9.5 shoots). After using untreated bacteria for plants' transformation, number of transgenic plants varied (dependently on cultivar) from 3.8 to 7.0/100 explants. Applying LB or MS as basic medium as well as adding tobacco plant extract to these media did not significantly influence transformation efficiency.Abbreviations: AS -acetosyringone; BA -benzyladenine; CTAB -hexadecyl trimethylammonium bromide; gus -ß-glucoronidase; IAA -indole-3-acetic acid; IBA -indole-3-butyric acid; LB -Luria Broth base; MS -Murashige and Skoog (1962); NOS -nopaline synthase promoter; nptII -neomycin phosphotransferase

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“…In terms of transformation, Scorza et al (1990b) reported Agrobacterium-mediated transformation of peach explants including leaf segments, immature embryos, and embryogenic callus. Ye et al (1994) demonstrated the feasibility of obtaining stable transgenic peach embryo callus tissue by biolistic transformation but no transgenic shoots were developed from this callus. There are only two reports of stable transgenic peach plant production.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of transformation in peach Prunus persica explants using green fluorescent protein (GFP) and beta-glucuronidase (GUS) reporter genes

Padilla

Golis

Gentile

et al. 2006

Plant Cell Tiss Organ Cult

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To determine the optimum conditions for Agrobacterium-mediated gene transfer, peach explants including cotyledons, embryonic axes and hypocotyl slices from non-germinated seeds and epicotyl internode slices from germinating seeds were exposed to Agrobacterium-mediated transformation treatments. The GUS (uidA) marker gene was tested using two different A. tumefaciens strains, three plasmids and four promoters [CaMV35s, (Aocs)3AmasPmas (''super-promoter''), mas-CaMV35s, and CAB]. GFP was tested with six A. tumefaciens strains, one plasmid (pLC101) and the doubleCaMV35s (dCaMV35s) promoter. The CaMV35s promoter produced more GUS expression than the CAB promoter. A. tumefaciens strains EHA105 and LBA4404 harboring the same plasmid (pBIN19) differed in their effects on GUS expression suggesting an interaction between A. tumefaciens strain and plasmid. A combination of A. tumefaciens EHA105, plasmid pBIN19 and the CaMV35s promoter produced the highest rates of transformation in peach epicotyl internodes (56.8%), cotyledons (52.7%), leaves (20%), and embryonic axes (46.7%) as evaluated by the percentage of explants expressing GUS 14 days after co-cultivation. GFP expression under the control of the dCaMV35s promoter was highest for internode explants but only reached levels of 18-19%. When GFP-containing plasmid pCL101 was combined with each of five A. tumefaciens strains the highest levels of transformation were 20-21% (internode and cotyledons, respectively). When nine peach genotypes were co-cultivated with A. tumefaciens strain EHA105 and GFP-containing plasmid pCL101 the highest levels of transformation were 26-28% (cotyledons and internodes, respectively). While GFP represents a potentially useful transformation marker that allows the non-destructive evaluation of transformation, rates of GFP transformation under the conditions of this study were low. It will be necessary to optimize expression of this marker gene in peach.Abbreviations: BA -benzyladenine; CAB -chlorophyll a/b binding protein promoter; CaMV35s -cauliflower mosaic virus promoter; GUS -beta-glucuronidase; LB -Luria Broth base; NPTII -neomycin phosphotransferase

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Genetic Transformation of Peach Tissues by Particle Bombardment

Cited by 52 publications

References 41 publications

Transformation of fruit trees. Useful breeding tool or continued future prospect?

Transformation of fruit trees. Useful breeding tool or continued future prospect?

Conditions of transformation and regeneration of `Induka' and `Elista' strawberry plants

Evaluation of transformation in peach Prunus persica explants using green fluorescent protein (GFP) and beta-glucuronidase (GUS) reporter genes

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