Genetic Transformation of<i>Coccidioides immitis</i>Facilitated by<i>Agrobacterium tumefaciens</i>

AbuOdeh, Raed; Orbach, Marc J.; Mandel, M. Alejandra; Das, Anath Bandhu; Galgiani, John N.

doi:10.1086/315525

Cited by 109 publications

(50 citation statements)

References 16 publications

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“…As a genus, Agrobacterium can transfer DNA to a remarkably broad group of organisms including numerous dicot and monocot angiosperm species (12,68,262,341) and gymnosperms (198,206,215,228,307,357,371). In addition, Agrobacterium can transform fungi, including yeasts (32,33,260), ascomycetes (1,71), and basidiomycetes (71). Recently, Agrobacterium was reported to transfer DNA to human cells (187).…”

Section: Agrobacterium "Species" and Host Rangementioning

confidence: 99%

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Gelvin

2003

Microbiol Mol Biol Rev

1,107

680

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SUMMARY Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Since the initial reports in the early 1980s using Agrobacterium to generate transgenic plants, scientists have attempted to improve this “natural genetic engineer” for biotechnology purposes. Some of these modifications have resulted in extending the host range of the bacterium to economically important crop species. However, in most instances, major improvements involved alterations in plant tissue culture transformation and regeneration conditions rather than manipulation of bacterial or host genes. Agrobacterium-mediated plant transformation is a highly complex and evolved process involving genetic determinants of both the bacterium and the host plant cell. In this article, I review some of the basic biology concerned with Agrobacterium-mediated genetic transformation. Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.

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Section: Agrobacterium "Species" and Host Rangementioning

confidence: 99%

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Gelvin

2003

Microbiol Mol Biol Rev

1,107

680

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“…Exogenous DNA sequences introduced into transferred DNA (T-DNA) vectors can be delivered to plants, making A. tumefaciens a cornerstone of plant genetic engineering. Under controlled conditions, A. tumefaciens can also transform mammalian cells and a variety of fungi, including the yeast Saccharomyces cerevisiae (1)(2)(3)(4)(5)(6).…”

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confidence: 99%

Purine synthesis and increasedAgrobacterium tumefacienstransformation of yeast and plants

Roberts

Metz

Monks

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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The bacterium Agrobacterium tumefaciens transforms eukaryotic hosts by transferring DNA to the recipient cell where it is integrated and expressed. Bacterial factors involved in this interkingdom gene transfer have been described, but less is known about host-cell factors. Using the yeast Saccharomyces cerevisiae as a model host, we devised a genetic screen to identify yeast mutants with altered transformation sensitivities. Twenty-four adenine auxotrophs were identified that exhibited supersensitivity to A. tumefaciens-mediated transformation when deprived of adenine. We extended these results to plants by showing that purine synthesis inhibitors cause supersensitivity to A. tumefaciens transformation in three plant species. The magnitude of this effect is large and does not depend on prior genetic manipulations of host cells. These data indicate the utility of yeast as a model for the transformation process and identify purine biosynthesis as a key determinant of transformation efficiency. These findings should increase the utility of A. tumefaciens in genetic engineering.A grobacterium tumefaciens, a Gram-negative soil bacterium, genetically transforms plants by transferring DNA to the host cell where it is integrated into the host chromosome and expressed. Exogenous DNA sequences introduced into transferred DNA (T-DNA) vectors can be delivered to plants, making A. tumefaciens a cornerstone of plant genetic engineering. Under controlled conditions, A. tumefaciens can also transform mammalian cells and a variety of fungi, including the yeast Saccharomyces cerevisiae (1-6).Understanding the cellular factors influencing transformation will provide broader insights into the mechanisms underlying interkingdom DNA transfer and should increase the utility of A. tumefaciens in genetic engineering. Bacterial factors that control virulence gene induction as well as processing and delivery of the T-DNA have been studied extensively (7,8). Recently, a few host-cell factors have been identified that participate in A. tumefaciens-mediated transformation. Studies in Arabidopsis thaliana have implicated histone H2A in chromosomal integration of the T-DNA (9). Studies in S. cerevisiae have implicated a nuclear pore protein in T-DNA nuclear import (10) and nonhomologous end-joining proteins in T-DNA chromosomal integration (11). To date, however, the facile yeast system has not been used to perform a large-scale screen to identify host factors that influence transformation sensitivity. Consequently, we devised a genetic screen to isolate yeast mutants with altered sensitivity to A. tumefaciens-mediated transformation. This approach revealed an unexpected link between transformation efficiency and de novo biosynthesis of adenine, an essential purine precursor of DNA, RNA, and ATP. Materials and MethodsStrains and Plasmids. The supervirulent A. tumefaciens strain EHA105 harboring pKP506 served as the bacterial donor strain in yeast-transformation experiments (1). The pKP506 plasmid contains the yeast TRP1 marker and the ARS1 rep...

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“…An important advance was the ability to introduce new genetic material into an organism by transformation, first achieved in C. albicans in 1986 (58) and later made possible in C. neoformans (37) and H. capsulatum (114), followed by C, glabrata (69), A. fumigatus (97), W. dermatitidis (82), and B. dermatitidis (55). The transformation of C. immitis was reported most recently (1,84). Although these transformation methods show some similarities, they differ in important aspects.…”

Section: Genetic Transformationmentioning

confidence: 99%

“…Electroporation also greatly increases the efficiency of transformation in H. capsulatum (113). The most unusual method of transformation is that developed by Abuodeh and coworkers, which employs Agrobacterium tumifaciens and its DNA transfer system to transform C. immitis (1).…”

Section: Genetic Transformationmentioning

confidence: 99%

“…Drug resistance markers are now available for seven of the major pathogenic fungi. Hygromycin resistance has been used to select transformants in C. neoformans (24), H. capsulatum (112), A. fumigatus (97), W. dermatitidis (82), C. immitis (1,84), and B. dermatitidis (55). Selection for nurseothricin resistance has recently been reported for C. neoformans (68).…”

Section: Genetic Transformationmentioning

confidence: 99%

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Molecular Genetic and Genomic Approaches to the Study of Medically Important Fungi

Magee

Gale²,

Berman

et al. 2003

Infect Immun

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Genetic Transformation ofCoccidioides immitisFacilitated byAgrobacterium tumefaciens

Cited by 109 publications

References 16 publications

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Purine synthesis and increasedAgrobacterium tumefacienstransformation of yeast and plants

Molecular Genetic and Genomic Approaches to the Study of Medically Important Fungi

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