Genetic transformation of germinated conidia of the thermophilic fungus Humicola grisea var. thermoidea to hygromycin B resistance

Abstract

Germinated conidia of the thermophilic fungus Humicola grisea var. thermoidea were transformed to hygromycin B resistance using the plasmid pAN7.1. Transformation was achieved using lithium acetate treatment or electroporation. The efficiency of transformation was up to 32 and 25 transformants per Wg of plasmid DNA with the two methods, respectively. Transformants obtained by the lithium acetate method were more stable and showed a high copy number of the hph gene integrated into their genome. The other transformants, from the electroporation procedure, were stable, but unable to grow in the presence of high levels of hygromycin, and detection of the hph gene was only possible by polymerase chain reaction analysis. z

Inflammatory Thermophilic Fungi are Used in Biotechnology Applications

“…using phleomycin resistance gene as a dominant selectable marker (51) and in Humicola grisea var. thermo idea using hygromycin B resistance gene as a selectable marker (52). Furthermore, a detailed study has been carried out on the amino acid sequence of ribosomal subunits of T lanuginosus and found that it is comparable to those sequences from other eukaryotic organisms indicating the common origin of ribosomal genes in these organisms.…”