Genetic transformation in the methanogen Methanococcus voltae PS

Bertani, G.; Baresi, Larry

doi:10.1128/jb.169.6.2730-2738.1987

Cited by 156 publications

(135 citation statements)

References 35 publications

Supporting

Mentioning

135

Contrasting

Order By: Relevance

“…Induction of the lytic cycle can be triggered by cellular or environmental cues [2,3] but also occurs spontaneously [1] at low levels within the bacterial population. While this is clearly bad news for the individual bacterium (now dead), low rates of phage lysis have been shown to increase the competitiveness of the surviving bacterial population.…”

Section: Temperate Phages As Weapons Of Bacterial Competitionmentioning

confidence: 99%

“…Prophage carriage is clearly accompanied by some jeopardy for the bacterium. Temperate phage can re-enter the lytic cycle spontaneously [1] or in response to cues from changing host or environmental conditions, [2,3] killing (with some exceptions) [4] the bacterial cell in the process. Since temperate phages are very abundant, [5][6][7] their ecological and evolutionary impacts on bacteria are likely to be important.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

2017

View full text Add to dashboard Cite

Infection by a temperate phage can lead to death of the bacterial cell, but sometimes these phages integrate into the bacterial chromosome, offering the potential for a more long-lasting relationship to be established. Here we define three major ecological and evolutionary benefits of temperate phage for bacteria: as agents of horizontal gene transfer (HGT), as sources of genetic variation for evolutionary innovation, and as weapons of bacterial competition. We suggest that a coevolutionary perspective is required to understand the roles of temperate phages in bacterial populations.

show abstract

Section: Temperate Phages As Weapons Of Bacterial Competitionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

2017

View full text Add to dashboard Cite

show abstract

“…40,41 Briefly, Escherichia coli DH5alpha cells were transformed with pBSK II. One colony was incubated overnight in 5 mL of Lysogeny broth (LB) medium medium 42,43 supplemented with 0.1 mg/mL of ampicillin at 37°C with aeration; and then 1 mL was inoculated in 300 mL of LB medium supplemented with 0.1 mg/mL of ampicillin and incubated for 8 h at 37°C with aeration. From these cultures plasmid DNA was purified using Qiagen Plasmid Maxi Prep Kit.…”

Section: Introductionmentioning

confidence: 99%

Electronic Structure and Spectro-Structural Correlations of Fe^IIIZn^II Biomimetics for Purple Acid Phosphatases: Relevance to DNA Cleavage and Cytotoxic Activity

et al. 2010

View full text Add to dashboard Cite

Purple acid phosphatases (PAPs) are a group of metallohydrolases that contain a dinuclear Fe III M II center (M II = Fe, Mn, Zn) in the active site and are able to catalyze the hydrolysis of a variety of phosphoric acid esters. The dinuclearhas recently been prepared and is found to closely mimic the coordination environment of the Fe III Zn II active site found in red kidney bean PAP (Neves et al. J. Am. Chem. Soc. 2007, 129, 7486). The biomimetic shows significant catalytic activity in hydrolytic reactions. By using a variety of structural, spectroscopic, and computational techniques the electronic structure of the Fe III center of this biomimetic complex was determined. In the solid state the electronic ground state reflects the rhombically distorted Fe III N 2 O 4 octahedron with a dominant tetragonal compression aligned along the μ-OH-Fe-O phenolate direction. To probe the role of the Fe-O phenolate bond, the phenolate moiety was modified to contain electron-donating or -withdrawing groups (-CH 3 , -H, -Br, -NO 2 ) in the 5-position. The effects of the substituents on the electronic properties of the biomimetic complexes were studied with a range of experimental and computational techniques. This study establishes benchmarks against accurate crystallographic structural information using spectroscopic techniques that are not restricted to single crystals. Kinetic studies on the hydrolysis reaction revealed that the phosphodiesterase activity increases in the order -NO 2 rBr rH rCH 3 when 2,4-bis(dinitrophenyl)phosphate (2,4-bdnpp) was used as substrate, and a linear free energy relationship is found when log(k cat /k 0 ) is plotted against the Hammett parameter σ. However, nuclease activity measurements in the cleavage of double stranded DNA showed that the complexes containing the electron-withdrawing -NO 2 and electron-donating -CH 3 groups are the most active while the cytotoxic activity of the biomimetics on leukemia and lung tumoral cells is highest for complexes with electron-donating groups.

show abstract

“…All plasmids were constructed and maintained in Escherichia coli recA strains prior to electroporation into the appropriate S. typhimurium strain. Cells were grown with aeration at 37°C in LB (29).…”

Section: Methodsmentioning

confidence: 99%

DNA Binding Is Not Sufficient for H-NS-mediated Repression ofproU Expression

Jordi¹,

Fielder

Burns

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

H-NS is a major component of bacterial chromatin and influences the expression of many genes. H-NS has beenshown to exhibit a binding preference for certain ATrich curved DNA elements in vitro. In this study we have addressed the factors that determine the specificity of H-NS action in vitro and in vivo. In bandshift studies, H-NS showed a slight binding preference for all curved sequences tested whether GC-based or AT-based; the specific architecture of the curve also influenced H-NS binding. In filter retention assays little difference in affinity could be detected for any sequence tested, including the downstream regulatory element (DRE) a downstream curved DNA element required for H-NS to repress transcription of the Salmonella typhimurium proU operon in vivo. A K d of 1-2 M was estimated for binding of H-NS to each of these sequences. In vivo, the distance between the proU promoter and the DRE, their relative orientations on the face of the DNA helix, and translation of the DRE had no major effect on proU regulation. None of the synthetic curved sequences tested could functionally replace the DRE in vivo. These data show that differential binding to curved DNA cannot account for the specificity of H-NS action in vivo. Furthermore, binding of H-NS to DNA per se is insufficient to repress the proU promoter. Thus, the DRE does not simply act as an H-NS binding site but must have a more specific role in mediating H-NS regulation of proU transcription.

show abstract

Genetic transformation in the methanogen Methanococcus voltae PS

Cited by 156 publications

References 35 publications

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

Electronic Structure and Spectro-Structural Correlations of Fe^IIIZn^II Biomimetics for Purple Acid Phosphatases: Relevance to DNA Cleavage and Cytotoxic Activity

DNA Binding Is Not Sufficient for H-NS-mediated Repression ofproU Expression

Contact Info

Product

Resources

About

Genetic transformation in the methanogen Methanococcus voltae PS

Cited by 156 publications

References 35 publications

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

Electronic Structure and Spectro-Structural Correlations of FeIIIZnII Biomimetics for Purple Acid Phosphatases: Relevance to DNA Cleavage and Cytotoxic Activity

DNA Binding Is Not Sufficient for H-NS-mediated Repression ofproU Expression

Contact Info

Product

Resources

About

Electronic Structure and Spectro-Structural Correlations of Fe^IIIZn^II Biomimetics for Purple Acid Phosphatases: Relevance to DNA Cleavage and Cytotoxic Activity