“…It is not the intention of this review to give a fully detailed compilation of the biology of transformation and the regulation of competence genes, although general aspects are discussed. Details may be found in a number of excellent overviews (79,80,251,325,343,344). The aim of this review is to give an overview of the research concepts, experimental strategies, and findings which relate to the bacterial gene flux by natural genetic transformation in several bacterial habitats, especially soil, sediment, and water.…”
Section: Gene Transfer Processes In the Environmentmentioning
confidence: 99%
“…on August 21, 2020 by guest http://mmbr.asm.org/ Downloaded from features of the other. For references on details of the transformation of B. subtilis, S. pneumoniae, and H. influenzae, the reader is referred to the previous reviews on the topic (79,80,104,251,325,343,344). Pertinent studies more recently published will be considered.…”
“…It is not the intention of this review to give a fully detailed compilation of the biology of transformation and the regulation of competence genes, although general aspects are discussed. Details may be found in a number of excellent overviews (79,80,251,325,343,344). The aim of this review is to give an overview of the research concepts, experimental strategies, and findings which relate to the bacterial gene flux by natural genetic transformation in several bacterial habitats, especially soil, sediment, and water.…”
Section: Gene Transfer Processes In the Environmentmentioning
confidence: 99%
“…on August 21, 2020 by guest http://mmbr.asm.org/ Downloaded from features of the other. For references on details of the transformation of B. subtilis, S. pneumoniae, and H. influenzae, the reader is referred to the previous reviews on the topic (79,80,104,251,325,343,344). Pertinent studies more recently published will be considered.…”
“…on December 13, 2020 by guest http://mmbr.asm.org/ Downloaded from features of the other. For references on details of the transformation of B. subtilis, S. pneumoniae, and H. influenzae, the reader is referred to the previous reviews on the topic (79,80,104,251,325,343,344). Pertinent studies more recently published will be considered.…”
Natural genetic transformation is the active uptake of free DNA by bacterial cells and the heritable incorporation of its genetic information. Since the famous discovery of transformation in Streptococcus pneumoniae by Griffith in 1928 and the demonstration of DNA as the transforming principle by Avery and coworkers in 1944, cellular processes involved in transformation have been studied extensively by in vitro experimentation with a few transformable species. Only more recently has it been considered that transformation may be a powerful mechanism of horizontal gene transfer in natural bacterial populations. In this review the current understanding of the biology of transformation is summarized to provide the platform on which aspects of bacterial transformation in water, soil, and sediments and the habitat of pathogens are discussed. Direct and indirect evidence for gene transfer routes by transformation within species and between different species will be presented, along with data suggesting that plasmids as well as chromosomal DNA are subject to genetic exchange via transformation. Experiments exploring the prerequisites for transformation in the environment, including the production and persistence of free DNA and factors important for the uptake of DNA by cells, will be compiled, as well as possible natural barriers to transformation. The efficiency of gene transfer by transformation in bacterial habitats is possibly genetically adjusted to submaximal levels. The fact that natural transformation has been detected among bacteria from all trophic and taxonomic groups including archaebacteria suggests that transformability evolved early in phylogeny. Probable functions of DNA uptake other than gene acquisition will be discussed. The body of information presently available suggests that transformation has a great impact on bacterial population dynamics as well as on bacterial evolution and speciation.
“…The transfer of the cellulase gene from E. coli was carried out by bacterial conjugation process described by Notani et al 13 with a few modifications. 13 After one day incubation of the separated culture of donor and recipient strains, samples were mixed (1:1) and 25µl of the mixture were spread onto 30mm of 0.45µm sterile millipore filter paper. After 24hr incubation, the filter was removed and put it into 10ml of LB broth and vortexed thoroughly until all the bacteria were off the filter.…”
Materials and methods
Sample collection, media and growth conditionBacterial strains were isolated from rice rhizospheric soil around Patheingyi Township, Mandalay Region, Myanmar and inoculated in the Glucose-Nitrogen Free Mineral Medium (GNFMM) at 37°C. The compositions of G-NFMM are: KH 2 PO 4 1.0g, MgSO 4 .7H 2 O 0.25g, NaCl 0.5g, FeSO 4 .7H 2 O 0.01g, MnSO 4 .H 2 O 0.01g, Na 2 MoO 4 0.01g,
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