Genetic transformation and evaluation of two sweet sorghum genotypes for resistance to spotted stemborer, Chilo partellus (Swinhoe)

Visarada, K. B. R. S.; Prasad, G. Shyam; Royer, Monique

doi:10.1007/s11816-016-0404-9

Cited by 15 publications

(10 citation statements)

References 17 publications

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“…In addition, a key aspect of the WU regeneration protocol was the use of ABA. A survey of various sorghum somatic embryogenesis studies [27,30,46,48,49,[52][53][54][55][56] indicated that the inclusion of auxin(s) and cytokinin(s) are standard for regeneration, but few use ABA for embryo maturation/ regeneration [49,53,55]. ABA is known to promote better embryogenic responses, with higher quality embryo formation, and subsequent plantlet conversion [57], so the superior performance of explants with the WU protocol may also reflect the inclusion of ABA during regeneration.…”

Section: In Vitro Performancementioning

confidence: 99%

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Flinn

Dale

Disharoon

et al. 2020

Plants

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Sorghum has been considered a recalcitrant plant in vitro and suffers from a lack of regeneration protocols that function broadly and efficiently across a range of genotypes. This study was initiated to identify differential genotype-in vitro protocol responses across a range of bioenergy sorghum parental lines and the common grain sorghum genotype Tx430 in order to characterize response profiles for use in future genetic studies. Two different in vitro protocols, LG and WU, were used for comparisons. Distinct genotype-protocol responses were observed, and the WU protocol performed significantly better for plantlet regeneration. Most bioenergy genotypes performed as well, if not better than Tx430, with Rio and PI329311 as the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic exudation responses, as indicated by medium browning. During the callus induction phase, genotypes prone to medium browning exhibited a response on WU medium which was either equal or greater than on LG medium. Genotype- and protocol-dependent albino plantlet regeneration was also noted, with three of the bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were susceptible to albino plantlet regeneration, with the response strongly associated with the WU protocol. These bioenergy parental genotypes, and their differential responses under two in vitro protocols, provide tools to further explore and assess the role of genetic loci, candidate genes, and allelic variants in the regulation of in vitro responsiveness in sorghum.

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Section: In Vitro Performancementioning

confidence: 99%

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Flinn

Dale

Disharoon

et al. 2020

Plants

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“…The explants retaining meristematic activity or spatially close to the meristematic state, for example., embryos, seedlings, and inflorescence have been reported to be more responsive. Immature embryos are most widely used for embryogenic callus formation and are shown to give highest transformation efficiency [ 137 , 138 ]. The source of embryos also has a significant impact on transformation efficiency.…”

Section: In Vitro Regeneration and Genetic Transformation Of Sweet Somentioning

confidence: 99%

Sweet sorghum as biofuel feedstock: recent advances and available resources

Mathur

Umakanth

Tonapi

et al. 2017

Biotechnol Biofuels

172

112

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Sweet sorghum is a promising target for biofuel production. It is a C4 crop with low input requirements and accumulates high levels of sugars in its stalks. However, large-scale planting on marginal lands would require improved varieties with optimized biofuel-related traits and tolerance to biotic and abiotic stresses. Considering this, many studies have been carried out to generate genetic and genomic resources for sweet sorghum. In this review, we discuss various attributes of sweet sorghum that make it an ideal candidate for biofuel feedstock, and provide an overview of genetic diversity, tools, and resources available for engineering and/or marker-assisting breeding of sweet sorghum. Finally, the progress made so far, in identification of genes/quantitative trait loci (QTLs) important for agronomic traits and ongoing molecular breeding efforts to generate improved varieties, has been discussed.

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“…In addition, a key aspect of the WU regeneration protocol was the use of ABA. A survey of various sorghum somatic embryogenesis studies (Elkonin et al 1995, Kaeppler and Pedersen 1997, Seetharama et al 2000, Gurel et al 2012, Liu and Godwin 2012, Assem et al 2014, Wu et al 2014, Do et al 2016, Visarada et al 2016, Belide et al 2017) indicated that the inclusion of auxin(s) and cytokinin(s) are standard for regeneration, but few use ABA for embryo maturation/regeneration (Assem et al 2014, Wu et al 2014, Belide et al 2017). ABA is known to promote better embryogenic responses, with higher quality embryo formation, and subsequent plantlet conversion (Merkle et al 1995), so the superior performance of explants with the WU protocol may also reflect the inclusion of ABA during regeneration.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of In Vitro Responses and Regeneration Between Diverse Bioenergy Sorghum Genotypes

Flinn

Dale

Disharoon

et al. 2019

Preprint

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25Sorghum has been considered a recalcitrant plant in vitro, and suffers from a lack of 26 regeneration protocols that function broadly and efficiently across a range of genotypes. This study 27 was initiated to identify differential genotype-in vitro protocol responses across a range of 28 bioenergy sorghum bioenergy parental lines, in order to characterize response profiles for use in 29 future genetic studies. Seven bioenergy sorghum genotypes were compared, along with the 30 common grain sorghum genotype Tx430, for their in vitro regeneration responses using two 31 different in vitro protocols, LG and WU. All genotypes displayed some level of response during 32 in vitro culture with both protocols. Distinct genotype-protocol responses were observed, with the 33 WU protocol significantly better for plantlet regeneration. All bioenergy genotypes, with the 34 exception of Chinese Amber, performed as well, if not better than Tx430, with Rio and PI329311 35 the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic 36 exudation responses, as indicated by medium browning. During the callus induction phase, 37 genotypes prone to medium browning exhibited a response on WU medium which was either equal 38 or greater than on LG medium, with Pink Kafir and PI329311 the most prone to medium browning. 39 Genotype-and protocol-dependent albino plantlet regeneration was also noted, with three of the 40 bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were 41 susceptible to albino plantlet regeneration, with the response strongly associated with the WU 42 protocol. Pink Kafir displayed the highest albino formation, with close to 25% of regenerating 43 explants forming albino plantlets. 44 45 46 47 48 49 50 51 52 53 Sorghum [Sorghum bicolor (L.) Moench] ranks fifth of the major grain crops in production, 54 area harvested, and yield worldwide (FAOSTAT Database 2017), and more than 300 million 55 people use it as a staple food, particularly in developing semiarid tropical regions (Kebede et al.56

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Genetic transformation and evaluation of two sweet sorghum genotypes for resistance to spotted stemborer, Chilo partellus (Swinhoe)

Cited by 15 publications

References 17 publications

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Sweet sorghum as biofuel feedstock: recent advances and available resources

Comparative Analysis of In Vitro Responses and Regeneration Between Diverse Bioenergy Sorghum Genotypes

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