Genetic transfer of lithoautotrophy mediated by a plasmid-cointegrate from Pseudomonas facilis

Warrelmann, Jürgen; Friedrich, Bärbel

doi:10.1007/bf00406565

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…Partially comparable situations may prevail in other facultative autotrophs. The hydra gen-oxidizing bacterium Pseudomonasfacilis strain K harbors megaplasmid pHG22-a that exclusively carries the cfx and box genes of the organism [20]. Also, in another hydrogen bacterium, Nocardia opaca, both markers of lithoautotrophy are encoded on a conjugative genetic element [21] which was recently identified to he a linear plasmid [27].…”

Section: Organization Of Cfx Genesmentioning

confidence: 99%

Genetics of CO2 fixation in the chemoautotroph Alcaligenes eutrophus

Bowien

1990

FEMS Microbiology Letters

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Section: Organization Of Cfx Genesmentioning

confidence: 99%

Genetics of CO2 fixation in the chemoautotroph Alcaligenes eutrophus

Bowien

1990

FEMS Microbiology Letters

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“…Instead, the proteins of A. eutrophus, Rhodobacter sphaeroides, and X. flavus, which belong to the ␣ and ␤ subdivisions of the proteobacteria, have the protein from (3,7,33,41). Plasmids harboring cbb genes which can be transferred via conjugation to other bacteria are frequently encountered in gram-negative bacteria (12,16,38,52). These mobile genetic elements in bacteria provide a means via which lateral transfer of autotrophic genes could have occurred.…”

mentioning

confidence: 99%

Primary structure and phylogeny of the Calvin cycle enzymes transketolase and fructosebisphosphate aldolase of Xanthobacter flavus

et al. 1996

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Xanthobacter flavus grows autotrophically by using the Calvin cycle for the fixation of CO 2 . Only 2 of the 11 enzymes of the Calvin cycle are characteristic for this pathway; the others are also present during heterotrophic growth. The key enzymes of the Calvin cycle, phosphoribulokinase and ribulosebisphosphate carboxylase, are encoded within the cbb operon, which is transcribed only during autotrophic growth. Two additional genes are located within this operon: cbbX, encoding a protein with unknown function, and cbbF, encoding fructosebisphosphatase (27,29). The transcription of the cbb operon is positively regulated by CbbR, a LysR-type transcriptional regulator, which binds to two sites in the cbb promoter (47).During autotrophic growth, X. flavus uses two fructosebisphosphatase enzymes with distinct properties. The inducible enzyme encoded by cbbF has a high level of sedoheptulosebisphosphatase activity and is stimulated by ATP. The second constitutive fructosebisphosphatase has a low level of sedoheptulosebisphosphatase activity and is not stimulated by ATP (48). In contrast to the fructosebisphosphatase isoenzyme pair, only one phoshoglycerate kinase gene, which is not encoded within the cbb operon, is employed by X. flavus. The pgk gene is constitutively expressed, but the expression level is higher during autotrophic growth than during heterotrophic growth (26).Little is known about the transketolase (EC 2.2.1.1) and fructosebisphosphate aldolase (FBP aldolase; EC 4.1.2.13) enzymes of X. flavus. Like fructosebisphosphatase and phosphoglycerate kinase, these enzymes are involved in both heterotrophic metabolism and the fixation of CO 2 via the Calvin cycle. Two unrelated mechanistically distinct types of FBP aldolase enzymes are encountered in bacteria, archaea, and eukarya (24). Class I FBP aldolases form a covalent Schiff base between the substrate and the ␣-amino group of a lysine residue during catalysis, whereas the class II enzymes depend on a divalent cation as the electrophile in the catalytic cycle (24).X. flavus (Table 1) was grown heterotrophically on succinate (10 mM) or gluconate (10 mM) and autotrophically on methanol (0.5% [vol/vol]) at 30ЊC as described previously (22,28). The activities of transketolase and FBP aldolase were determined, according to published methods (15, 49), in cell extracts which were prepared by using a French pressure cell as described previously (27). The activity of transketolase was increased sixfold following autotrophic growth on methanol compared with that of heterotrophically grown cells. In sharp contrast, the activity of FBP aldolase (without Fe 2ϩ ) was the same for both heterotrophic and autotrophic growth. Because the activity of class II FBP aldolase is dependent on Fe 2ϩ as the electrophile (24), FeSO 4 (700 M) was included in the reaction assay. Surprisingly, Fe 2ϩ did not affect the FBP aldolase activity in the cell extracts of heterotrophically grown cells but stimulated the activity of FBP aldolase in cell extracts of autotrophically grown cells ...

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“…Also, RubisCO genes are reportedly located on the chromosome as well as on plasmid pPB13 of the nitrite‐oxidizing nitrifier Nitrobacter hamburgensis X 14 [42]. The hydrogen oxidizer Acidovorax facilis K carries the cbb genes exclusively on plasmid pHG22‐a, whereas strain J of the same species only has chromosomal cbb genes [43]. An exclusive plasmid location of these genes has also been demonstrated in the sole Gram‐positive bacterium studied so far in this respect, the hydrogen oxidizer Rhodococcus opacus .…”

Section: Organization and Genomic Location Of Cbb Genesmentioning

confidence: 99%

Organization and regulation of cbb CO2 assimilation genes in autotrophic bacteria

Kusian¹,

Bowien²

2006

FEMS Microbiology Reviews

132

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The Calvin-Benson-Bassham cycle constitutes the principal route of CO2 assimilation in aerobic chemoautotrophic and in anaerobic phototrophic purple bacteria. Most of the enzymes of the cycle are found to be encoded by cbb genes. Despite some conservation of the internal gene arrangement cbb gene clusters of the various organisms differ in size and operon organization. The cbb operons of facultative autotrophs are more strictly regulated than those of obligate autotrophs. The major control is exerted by the cbbR gene, which codes for a transcriptional activator of the LysR family. This gene is typically located immediately upstream of and in divergent orientation to the regulated cbb operon, forming a control region for both transcriptional units. Recent studies suggest that additional protein factors are involved in the regulation. Although the metabolic signal(s) received by the regulatory components of the operons is (are) still unknown, the redox state of the cell is believed to play a key role. It is proposed that the control of the cbb operon expression is integrated into a regulatory network.

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Genetic transfer of lithoautotrophy mediated by a plasmid-cointegrate from Pseudomonas facilis

Cited by 8 publications

References 31 publications

Genetics of CO2 fixation in the chemoautotroph Alcaligenes eutrophus

Genetics of CO2 fixation in the chemoautotroph Alcaligenes eutrophus

Primary structure and phylogeny of the Calvin cycle enzymes transketolase and fructosebisphosphate aldolase of Xanthobacter flavus

Organization and regulation of cbb CO2 assimilation genes in autotrophic bacteria

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