Genetic transduction by phages and chromosomal islands: The new and noncanonical

Chiang, Yin Ning; Penadés, José R.; Chen, John

doi:10.1371/journal.ppat.1007878

Cited by 137 publications

(114 citation statements)

References 27 publications

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“…During the lytic cycle, many bacteriophages are capable of generalized transduction , during which they pick up and package chromosomal or plasmid DNA from the host at a low frequency [7]. Many members of the Caudovirales —the temperate phages—are capable of undergoing lysogeny , during which their genomes are integrated as prophages into their host genomes.…”

Section: Introductionmentioning

confidence: 99%

“…Temperate phages sometimes pick up adjacent genetic material during the process of excision (usually referred to as specialized transduction ). A related process termed lateral transduction involves in situ replication and packaging and is capable of packaging DNA several thousands of base pairs downstream of the prophage [7]. In many cases, prophages lose their ability to excise and become cryptic or defective phages, permanently entombed within their host genomes [8].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Piracy: Redirection of Bacteriophage Capsid Assembly by Mobile Genetic Elements

Dokland

2019

Viruses

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Horizontal transfer of mobile genetic elements (MGEs) is a key aspect of the evolution of bacterial pathogens. Transduction by bacteriophages is especially important in this process. Bacteriophages—which assemble a machinery for efficient encapsidation and transfer of genetic material—often transfer MGEs and other chromosomal DNA in a more-or-less nonspecific low-frequency process known as generalized transduction. However, some MGEs have evolved highly specific mechanisms to take advantage of bacteriophages for their own propagation and high-frequency transfer while strongly interfering with phage production—“molecular piracy”. These mechanisms include the ability to sense the presence of a phage entering lytic growth, specific recognition and packaging of MGE genomes into phage capsids, and the redirection of the phage assembly pathway to form capsids with a size more appropriate for the size of the MGE. This review focuses on the process of assembly redirection, which has evolved convergently in many different MGEs from across the bacterial universe. The diverse mechanisms that exist suggest that size redirection is an evolutionarily advantageous strategy for many MGEs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Piracy: Redirection of Bacteriophage Capsid Assembly by Mobile Genetic Elements

Dokland

2019

Viruses

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“…For phage transduction, the DNA transfer is mediated by bacteriophages, which are viruses that can infect bacteria and manipulate the bacterial replication, transcription, and translation machineries to drive their own proliferation. Phages that infect LAB have been thoroughly investigated, and different species of LAB have been screened for susceptibility to specific phages [17][18][19][20][21]. The third pathway of natural competence in prokaryotes is a developmental process in which exogenous DNA is translocated through the native DNA-uptake machinery.…”

Section: Natural Methodsmentioning

confidence: 99%

Safety Aspects of Genetically Modified Lactic Acid Bacteria

Plavec

Berlec

2020

Microorganisms

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Lactic acid bacteria (LAB) have a long history of use in the food industry. Some species are part of the normal human microbiota and have beneficial properties for human health. Their long-standing use and considerable biotechnological potential have led to the development of various systems for their engineering. Together with novel approaches such as CRISPR-Cas, the established systems for engineering now allow significant improvements to LAB strains. Nevertheless, genetically modified LAB (GM-LAB) still encounter disapproval and are under extensive regulatory requirements. This review presents data on the prospects for LAB to obtain ‘generally recognized as safe’ (GRAS) status. Genetic modification of LAB is discussed, together with problems that can arise from their engineering, including their dissemination into the environment and the spread of antibiotic resistance markers. Possible solutions that would allow the use of GM-LAB are described, such as biocontainment, alternative selection markers, and use of homologous DNA. The use of GM-LAB as cell factories in closed systems that prevent their environmental release is the least problematic aspect, and this is also discussed.

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“…It is worth noting that gene transfer can also be mediated by phageresembling particles encoded by bacterial chromosomes, called Gene Transfer Agents (GTAs; Grüll et al, 2018). Lately, a new phenomenon called "lateral transduction, " initially described in Staphylococcus aureus prophages, seems to be involved in gene transfers in a wide range of bacterial species Chiang et al, 2019). Briefly, upon entry in lytic state, some prophages do not start their activation by an excision from the chromosome but undergo an in situ genome replication.…”

Section: Bacteriophage-assisted Dna Transfermentioning

confidence: 99%

Cell-Free DNA: An Underestimated Source of Antibiotic Resistance Gene Dissemination at the Interface Between Human Activities and Downstream Environments in the Context of Wastewater Reuse

2020

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Genetic transduction by phages and chromosomal islands: The new and noncanonical

Cited by 137 publications

References 27 publications

Molecular Piracy: Redirection of Bacteriophage Capsid Assembly by Mobile Genetic Elements

Molecular Piracy: Redirection of Bacteriophage Capsid Assembly by Mobile Genetic Elements

Safety Aspects of Genetically Modified Lactic Acid Bacteria

Cell-Free DNA: An Underestimated Source of Antibiotic Resistance Gene Dissemination at the Interface Between Human Activities and Downstream Environments in the Context of Wastewater Reuse

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