Genetic techniques for control of sexuality in fish farming

Purdom, C. E.

doi:10.1007/bf02264069

Cited by 14 publications

(7 citation statements)

References 12 publications

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“…For a t least two reasons, gynogenesis (like direct ocstrogcn treatment) may not be the best available method for producing monosex female yellow perch and walleye for aquaculture. First, in several fish species, the performance of gynogens has been shown to be negatively affected by increased homozygosity (Onozato 1984;Streisinger et al 1981;Purdom 1986). Second, the use of heat or hydrostatic pressure shocks has been shown to have a negative effect on the survival and growth of juvenile yellow perch (see the section on direct triploidy induction below).…”

Section: Production Of Monosex Female Populations By Gynogenesismentioning

confidence: 99%

“…Dissection of a partially sex-inverted genetic female walleye that had been treated with 17~-methyltestosterone at 50 mg/kg &et for 60 days, beginning when the fish was 50-70 mm total length. This fish contained one apparently normal ovary and one testes that was filled with viable spermatozoa differential egg quality and susceptibility of eggs from different origins to heat shocks (Lou and Purdom 1984;Purdom 1986;Johnstone 1987;Allen and Bushek 1992). In yellow perch, the wide range of water temperatures from which brood fish are captured may be one of the factors that results in such variation (Malison et al 1993a).…”

Section: Direct Triploidy Inductionmentioning

confidence: 99%

“…For example, they would allow for stocking into lakes without risking permanent introduction or altering the genetic integrity and overall fitness of wild fish stocks (Thorgaard and Allen 1987). Methods of controlling sex and inducing sterility have been widely explored to improve growth and production in various fish species (see the reviews by Bye and Lincoln 1986;Ihssen et al 1990;Purdom 1986). The remainder of this paper will review methods of producing monosex female and sterile populations of yellow perch and walleye.…”

Section: Introductionmentioning

confidence: 99%

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Sex control and ploidy manipulations in yellow perch (Perca flavescens) and walleye (Stizostedion vitreum)

Malison

Garcia‐Abiado

1996

J Appl Ichthyol

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In both yellow perch (Percu ,flavescens) and wallcyc (Stizostedion citreurn), remales grow significantly faster and reach a larger ultimate size than males. In addition, reproductive development in both of these species can have a significant negative impact on somatic growth and fillet yield. Accordingly, methods for producing monosex female populations and for inducing sterility, have important potential applications for both commercial fish culture and fisheries management. Of the several available methods for producing monosex female populations in fishes (such as yellow perch and walleye) in which females are homogametic, the preferred method (described herein) may be to treat juveniles with androgens to induce phenotypic sex inversion of genetic females, and to subsequently use sperm from these females to fertilize normal eggs. Initial efforts at inducing sterility focused on the direct usc of cithcr heat or hydrostatic pressure shocks to produce triploid yellow perch and walleye. The gonadal development of triploid yellow perch and walleye of both sexes is retarded compared to that of diploids, and triploid yellow perch can have higher fillet yields than diploids. The direct use of heat and pressure shocks to induce triploidy in yellow perch, however, has negative effects on growth that are independent of ploidy status. One way to circumvent this problem is to produce triploids by crossing fertile tetraploids with diploids. To date, methods of producing viable tetraploids (beyond the larval stage) have been developed for yellow perch but not for walleye.

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Section: Production Of Monosex Female Populations By Gynogenesismentioning

confidence: 99%

Section: Direct Triploidy Inductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sex control and ploidy manipulations in yellow perch (Perca flavescens) and walleye (Stizostedion vitreum)

Malison

Garcia‐Abiado

1996

J Appl Ichthyol

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“…The techniques are relatively simple with two steps: generally, inactivation of spermatozoa by UV irradiation or chemical treatments and fertilized egg shock treatments (thermal or pressure) for diploidization of maternal chromosome set (Ghigliotti, Bolla, Duc, Ottesen & Babiak, ; Lebeda, Dzyuba, Rodina & Flajshans, ; Nowosad, Kucharczyk, Liszewski, Targońska & Kujawa, ; You, Yu, Tan & Tong, ). Artificial gynogenesis has been widely used in the study of fish genetics and breeding like for the production of monosex fish (Luo et al., ; Purdom, ), establishment of inbred lines (Li, Liang, Luo, Pan & Zou, ) and identification of the sex determination system (Campos‐Ramos, Harvey, Mcandrew & Penman, ; Chen et al., ; Fopp‐Bayat, ; Hassanzadeh Saber & Hallajian, ; Li et al., ; Luo et al., ; Purdom, ).…”

Section: Introductionmentioning

confidence: 99%

Induction of meiotic gynogenesis in bagrid catfish (Pseudobagrus ussuriensis) with homologous sperm and its confirmation for female homogamety

et al. 2017

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The bagrid catfish, Pseudobagrus ussuriensis, exhibits significant sexual dimorphism in growth rate and body size with males growing faster than females. Therefore, an all‐male culture can dramatically increase the output and profitability of fishery products. According to the monosex breeding route, super‐male individuals’ acquirement by XY male sex reversal and artificial gynogenesis is the key step. An effective protocol to induce meiotic gynogenesis using homologous sperms has been developed in this study. The most effective UV irradiation for sperm genetic inactivation was found to be at a distance of 20 cm with 66 μW/cm2 light intensity for 25 min. Optimal treatment for cold shock was 5 min post‐fertilization at 0‐4°C for 30 min, which gave the best survival rate of 13.65 ± 2.87%. The sex ratio in the meiotic gynogens showed a significant female‐biased deviation (p < .05); thirty meiogynogens and their parents were further analysed using a male‐specific AFLP marker, of which only the male parent produced a male‐specific DNA band of 412 bp. These results indicated the female homogametic XX/XY sex determination system in P. ussuriensis. The optimization of a protocol for the successful induction of meiogynogenesis in the bagrid catfish lays the basis for all‐male production and is useful in ascertaining the genetic sex determination system in this promising aquaculture species.

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“…Triploidy and other chromosome set manipulations are widely used in commercial aquaculture. The majority of salmonids produced in the United Kingdom are either all female or all female triploid fish (Purdom 1986). Also, triploid grass carp Ctenophuryngodon idella are used extensively in North America as a biological control agent for weeds (Cassani and Caton 1985).…”

mentioning

confidence: 99%

Pressure Induced Triploidy in Hybrid Lepomis

Wills

Paret

Sheehan

1994

J World Aquaculture Soc

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Worldwide expansion of aquaculture will require identification of species more adapted to localized conditions and less likely to affect genetic diversity of natural populations in the event of escape. Hybrids of Lepomis sunfish have the potential as an alternative for aquaculture in regions of North America where the growing season is unsuitable for production of traditional coldwater and warm‐water species. Triploidy was induced in hybrids of green sunfish Lepomis cyanellus males and bluegill L. macrochirus females using hydrostatic pressure shocks. A total of 27 combinations of pressure (41,369, 48,264, and 55,158 kPa), duration (2, 3, 4 min), and post‐fertilization time of shock initiation (2, 3, 4 min) were tested to identify those which were most efficient for inducing triploidy. Several of the shock regimes produced 100% triploid hybrid sunfish with at least 90% survival relative to unshocked controls. The two shock treatments deemed best overall were: 1) 48,264 kPa for 4 min begun 2 min after fertilization; and 2) 41,369 kPa for 2 min begun 3 min after fertilization.

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Genetic techniques for control of sexuality in fish farming

Cited by 14 publications

References 12 publications

Sex control and ploidy manipulations in yellow perch (Perca flavescens) and walleye (Stizostedion vitreum)

Sex control and ploidy manipulations in yellow perch (Perca flavescens) and walleye (Stizostedion vitreum)

Induction of meiotic gynogenesis in bagrid catfish (Pseudobagrus ussuriensis) with homologous sperm and its confirmation for female homogamety

Pressure Induced Triploidy in Hybrid Lepomis

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