Genetic studies on net blotch resistance in a barley cross

Ho, K. M.; Choo, T. M.; Tekauz, A.; Martin, Richard A.

doi:10.4141/cjps96-123

Cited by 52 publications

(37 citation statements)

References 12 publications

(8 reference statements)

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“…As the resistance to the two forms of net blotch is inherited independently (Ho et al ., 1996), it is important that the pathogen is correctly identified. The main aim of this study was focused on the development of specific primers that differentiate spot form and net form isolates of the net blotch fungus P. teres from each other and also from P. graminea.…”

Section: Introductionmentioning

confidence: 99%

AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

2005

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Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres , the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its differentiation from P. teres f. maculata (spot form), which is morphologically very similar to P. teres f. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungal mycelium. The PCR assay was validated with 60 samples of Pyrenophora species. The amplification with four designed PCR primer pairs provided P. teres form-specific products. No cross-reaction was observed with DNA of several other species, such as P. tritici-repentis , P. graminea and Helminthosporium sativum .

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Section: Introductionmentioning

confidence: 99%

AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

2005

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“…In Canada and Egypt, one dominant gene for resistance was detected (Buchannon and McDonald 1965), whereas in Australia, Khan and Boyd (1969) identified 2 dominant resistance genes. More recently, numerous genes were identified in different barley lines from around the world (Wilcoxson et al 1992;Douiyssi et al 1996;Ho et al 1996;Afanasenko et al 1999;Jonsson et al 1999). A current study of barley genotypes using NTNB isolates from Western Australia, Queensland, and South Australia, has detected more than 6 genes for resistance (Gupta et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.)

Çakır¹,

Gupta²,

Platz³

et al. 2003

Aust. J. Agric. Res.

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Abstract. Identification and deployment of disease resistance genes are key objectives of Australian barley breeding programs. Two doubled haploid (DH) populations derived from Tallon × Kaputar (TK) and VB9524 × ND11231 (VN) crosses were used to identify markers for net type net blotch (NTNB) (Pyrenophora teres f. teres). The maps included 263 and 250 markers for TK and VN populations, respectively. The TK population was screened with 5 pathotypes and the VN population with 1 pathotype of NTNB as seedlings in the glasshouse. In addition, the TK population was subjected to natural infection in the field at Hermitage Research Station, Qld. Analyses of the markers were performed using the software packages MapManager and Qgene. One region on chromosome 6H was strongly associated with resistance to NTNB in both populations (R 2 = 83% for TK and 66% for VN). In the TK population, 2 more quantitative trait loci (QTLs) were identified on chromosomes 2H and 3H, with R 2 values of 30% and 31%, respectively. These associations were consistent over all pathotypes studied during the seedling stage. The same QTL on chromosome 6H was also found to be highly significantly associated (R 2 = 65%) with the adult plant (field) response in the TK population. There are several very closely linked markers showing strong associations in these regions. Association of the 4 markers on chromosome 6H QTL with resistance to the NTNB has been validated in 2 other DH populations derived from barley crosses Pompadour × Stirling and WPG8412 × Stirling. These markers present an opportunity for marker assisted selection of lines resistant to NTNB in barley breeding programs.

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“…Furthermore, as proposed by Choo et al (1979) in a population improvement program for net blotch resistance, selection intensity could be higher because additive)additive epistasis for the evaluation parameters was predominantly complementary. Ho et al (1996) determined that resistance, under growth chamber conditions, to a specific isolate of P. teres (WRS102) at the three-leaf stage, was linked to the two-row phenotype on chromosome 2. Although the screening in this investigation was made at the adult plant stage, it is apparent that the two-row DH lines were associated with net blotch resistance at Mograne 2002Á2003 and Tunis greenhouse 2004 environments, but not at Mograne 2003Á2004 environment.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of spike type, which is controlled by a single gene, vrs1, (Vrs1Vrs1 for two-row type), on agronomic traits, chemical composition, and disease resistance was previously studied (Jui et al 1997;Fre´geau-Reid et al 2001;Choo et al 2004). For net blotch resistance, one resistance gene was shown to be linked to the Vrs1Vrs1 genotype on chromosome 2 (Ho et al 1996). However previously, Khan and Boyd (1969) reported that net blotch resistance segregates independently of spike type.…”

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confidence: 99%

Genetic analysis of net blotch resistance in a two-row × six-row cross of barley, using doubled-haploid lines

Chérif¹,

Rezgui²,

Devaux³

et al. 2008

Can. J. Plant Sci.

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Cherif, M., Rezgui, S., Devaux, P. and Harrabi, M. 2008. Genetic analysis of net blotch resistance in a two-row six-row cross of barley, using doubled-haploid lines. Can. J. Plant Sci. 88: 257Á266. The effect of spike type on net blotch resistance is not fully understood in barley (Hordeum vulgare L.). A doubled-haploid barley population derived from a cross between a susceptible two-row (Roho) and a resistant six-row (line 90) genotypes was used to study the genetics of net blotch resistance and to determine the effect of spike type on net blotch resistance at the adult plant growth stage across three environments. Net blotch rating was evaluated using a mass disease index, area under the disease progress curve and the apparent infection rate. Partial resistance to Pyrenophora teres was observed in many DH lines and in the parental line 90 in the three environments. This result indicated that selecting for partial resistance is feasible under severe net blotch conditions where differential responses among DH lines were noted as in Mograne in 2003Á2004. The normality test and/ or the means comparison method suggested that additive)additive epistasis effects influenced the expression of all disease parameters in the three environments. Both the coefficient of skewness and the number of transgressive lines indicate the presence of a complementary gene interaction for most disease parameters. Results showed that two-row lines were particularly associated with net blotch resistance in Mograne 2002Á2003 and in Tunis greenhouse 2004. A strategy for improving the level of quantitative resistance to P. teres in later generations is recommended. Cherif, M., Rezgui, S., Devaux, P. et Harrabi, M. 2008. Genetic analysis of net blotch resistance in a two-row six-row cross of barley, using doubled-haploid lines. Can. J. Plant Sci. 88: 257Á266. L'effet du nombre de rangs de l'e´pie´pi sur la re´sistancere´sistance de l'orge (Hordeum vulgare L.) a` la rayure re´ticuleére´ticuleé est mal compris. Une population haploı¨dehaploı¨de doubleé issue d'un croisement entre un cultivar a` deux rangs sensible (Roho) et une ligneé locale a` six rangs re´sistantere´sistante (ligneé 90) a e´teútiliseée´teútiliseé pour e´tudiere´tudier la ge´ne´tiquege´ne´ge´ne´tique de la re´sistancere´sistance et pour de´terminerde´terminer l'effet du nombre de rangs de l'e´pie´pi sur la re´sistancere´sistance a` la rayure re´ticuleére´ticuleé au stade adulte dans trois environnements. La reáction a` la rayure re´ticuleére´ticuleé a e´teé´valueée´teé´e´teé´valueé moyennant l'indice d'attaque, la surface au-dessous de la courbe d'e´volutione´volution de la maladie et le taux d'infection apparent. De nombreuses ligneés haploı¨dehaploı¨de-doubleés ainsi que la ligneé 90 ont manifesteúne re´sistancere´sistance quantitative a` Pyrenophora teres dans les trois environnements. Cette e´tudee´tude indique que la seíection pour la re´sistancere´sistance partielle a` la rayure re´ticuleére´ticuleé serait plus facile en conditions de forte e´pide´miee´pide´e´pide´mie,o...

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Genetic studies on net blotch resistance in a barley cross

Cited by 52 publications

References 12 publications

AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.)

Genetic analysis of net blotch resistance in a two-row × six-row cross of barley, using doubled-haploid lines

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