1984
DOI: 10.1007/bf00420225
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Genetic studies of purine breakdown in the fission yeast Schizosaccharomyces pombe

Abstract: Purines such as hypoxanthine, xanthine, uric acid, allantoin and allantoic acid serve as sole nitrogen sources for the yeast Schizosaccharomyces pombe. A number of classes of mutants unable to use purines have been isolated and genetically analysed. Mutants in the urol gene lack uricase, all1 lack allantoinase, ala1 lack allantoicase whilst in ure1, ure2, ure3 and ure4 genes lack urease activity. Mutants in four hyp genes are unable to convert hypoxanthine to uric acid whilst mutation in xan1 results in impair… Show more

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Cited by 17 publications
(19 citation statements)
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“…[7][8][9], and an essential role for auxiliary genes in forming active urease has been confirmed in these and many other bacteria. Genetic studies are consistent with a similar requirement for urease accessory genes in the fungi Neurospora crassa (10), Schizosaccharomyces pombe (11), and Aspergillus nidulans (12,13). Finally, two loci (Eu2 and Eu3) that appear to encode genes associated with urease maturation factors such as nickel emplacement have been identified in soybean plants (14).…”
supporting
confidence: 63%
“…[7][8][9], and an essential role for auxiliary genes in forming active urease has been confirmed in these and many other bacteria. Genetic studies are consistent with a similar requirement for urease accessory genes in the fungi Neurospora crassa (10), Schizosaccharomyces pombe (11), and Aspergillus nidulans (12,13). Finally, two loci (Eu2 and Eu3) that appear to encode genes associated with urease maturation factors such as nickel emplacement have been identified in soybean plants (14).…”
supporting
confidence: 63%
“…The addition of 0.1 mM Ni restored the ability of this mutant fungus to grow on urea and increased the urease activity to 5 to 8% of the wildtype levels, but Ni addition to cell extracts did not result in urease activity. Furthermore, the number of urease loci in Neurospora crassa (4) and Schizosaccharomyces pombe (11) exceeds that required for the urease structural genes. The function of the additional genes is unknown, but one could speculate that they function in Ni incorporation or other maturation event.…”
Section: Resultsmentioning
confidence: 99%
“…The four urease-negative mutants of S. pombe (ure1, ure2, ure3 and ure4) were a gift from Dr. R. Fluri (Kinghorn and Fluri 1984). All were leucine auxotrophs (leu1-32).…”
Section: Biological Materialsmentioning
confidence: 99%
“…Complementation of S. pombe urease mutants by genomic DNA bearing the putative accessory genes ureD, ureF and ureG Three non-allelic urease-negative mutants -ure1, ure3, and ure4 (Kinghorn and Fluri 1984;Lubbers et al 1997) -were transformed with a genomic DNA library constructed in the plasmid pDB248' (Beach et al 1982) and clones expressing the plasmid marker (LEU2) were selected on EMM minimal medium without leucine supplementation. Clones were then transferred to EMM lacking leucine, in which the nitrogen source was 5 mM urea (EMMU).…”
Section: Identification Of Putative Accessory Genes In S Pombementioning
confidence: 99%
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