Genetic structure of Muzzafarnagri sheep based on microsatellite analysis

Arora, Reena; Bhatia, Sabhyata

doi:10.1016/j.smallrumres.2003.11.015

Cited by 61 publications

(59 citation statements)

References 11 publications

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“…The total allele numbers, mean allele number per locus, effective number of alleles, and mean heterozygosity (0.87) observed in 17 microsatellite loci were higher than those reported in the literature (16)(17)(18)(19)(20)(21)(22)(23)(24). These findings indicate that the used microsatellite markers can be reliably used to measure genetic diversity for these breeds.…”

Section: Discussioncontrasting

confidence: 53%

Genetic relationships among four Turkish sheep breeds using microsatellites

Yılmaz¹,

Sezenler²,

Sevim³

et al. 2015

Turk J Vet Anim Sci

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IntroductionTurkey's proximity to domestication centers, along with its climate, topographical pattern, and agricultural structure that is formed according to plant cover, has created an appropriate environment for sheep breeding. Therefore, there are many sheep breeds in various regions and territories (1,2). Although there are many varieties and crossbred populations of sheep breeds in Turkey, 20 sheep breeds have been officially registered by the Ministry of Food, Agriculture, and Livestock (3). Even though Gökçeada (GA), Kıvırcık (KIV), Karacabey Merino (KM), and Sakız (SZ) sheep breeds, which are raised in the western Anatolian and Marmara regions, have a low percentage of native sheep population, each breed has certain significant characteristics. SZ sheep, known for their high fertility and milk production, and GA sheep, known for their milk production and high lamb survival rate, are raised along the coastline of the Aegean region. KIV and KM sheep breeds are known for their meat quality, wool, and meat production and are raised in western Turkey. For the purpose of improving the meat quality and fertility of the sheep population in western Turkey, the SZ and KIV breeds are commonly used in crossbreeding (4).Molecular genetic methods for identifying genetic structure and diversity in farm animals have shown a rapid development in recent years and have become widely used. Various molecular genetic methods have been developed for this purpose. Microsatellites that are specific to DNA regions are more widely used than other methods (5).This study aims to determine the genetic structure of GA and SZ sheep breeds, which are bred on the coastline of the Aegean region, and that of the KIV and KM sheep breeds, which are commonly bred in the Aegean and Marmara regions, by means of microsatellites. The information obtained from this study is based on microsatellite markers and will clarify the genetic relationships between these sheep breeds. It will also be helpful for determining current and future breeding programs and breed management and conservation strategies. Materials and methods Animal resources and DNA isolationThe animal material of the study consisted of a total of 250 animals belonging to GA, KIV, KM, and SZ sheep populations bred in the Aegean and Marmara regions. The origins and sample sizes of these 4 breeds are presented in Table 1. A DNA isolation kit (Applied Biological Materials Inc., Canada) was used for DNA extraction from blood samples.

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Section: Discussioncontrasting

confidence: 53%

Genetic relationships among four Turkish sheep breeds using microsatellites

Yılmaz¹,

Sezenler²,

Sevim³

et al. 2015

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

“…The importance of conservation of genetic diversity in livestock and the difficulty to assess the within and between populations genetic variability has led to consider many different genetic polymorphism based measures of genetic diversity [12]. Additionally, most of the studies published that use marker information to estimate genetic variability are focussed in the variability among breeds, populations or flocks, but few of them within breed [2,21]. In any case, it would be possible to make some comparisons indicating, for example, that the Kinship distance estimated in a previous study [1] in the Latxa Black Faced (0.354) is similar to the values here reported for the population of males born in 2002.…”

Section: Discussionmentioning

confidence: 99%

“…II) even though they are recommended for parentage verification [20] and sheep diversity measurement [13] and are highly variable in other sheep populations [2,10,21,32]. The resulting average number of alleles, expected heterozygosity and polymorphic information content were similar or higher than values obtained when characterising the genetic variability in other breeds and with other microsatellite panels [1-3, 10, 21, 27, 29].…”

Section: Genetic Polymorphism Parametersmentioning

confidence: 99%

The effects of selective breeding against scrapie susceptibility on the genetic variability of the Latxa Black-Faced sheep breed

et al. 2006

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-Breeding sheep populations for scrapie resistance could result in a loss of genetic variability. In this study, the effect on genetic variability of selection for increasing the ARR allele frequency was estimated in the Latxa breed. Two sources of information were used, pedigree and genetic polymorphisms (fifteen microsatellites). The results based on the genealogical information were conditioned by a low pedigree completeness level that revealed the interest of also using the information provided by the molecular markers. The overall results suggest that no great negative effect on genetic variability can be expected in the short time in the population analysed by selection of only ARR/ARR males. The estimated average relationship of ARR/ARR males with reproductive females was similar to that of all available males whatever its genotype: 0.010 vs. 0.012 for a genealogical relationship and 0.257 vs. 0.296 for molecular coancestry, respectively. However, selection of only ARR/ARR males implied important losses in founder animals (87 percent) and low frequency alleles (30 percent) in the ram population. The evaluation of mild selection strategies against scrapie susceptibility based on the use of some ARR heterozygous males was difficult because the genetic relationships estimated among animals differed when pedigree or molecular information was used, and the use of more molecular markers should be evaluated. genetic variability / scrapie / Manech / Latxa / sheep

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“…Microsatellite markers are currently the markers of choice for molecular genetic studies such as reconstruction of phylogenetic and relationships among populations (Bowcock et al, 1994;Forbes et al, 1995;MacHugh et al, 1997), determination of paternity and kinship analyses (Glowatzki-Mullis et al, 1995;Heyen et al, 1997;Luikart et al, 1999;Schlotterer, 2004) forensic studies (Edwards et al, 1992), linkage analysis (Francisco et al, 1996;Kappes et al, 1997;Mellersh et al, 1997), population structures (Arora and Bhatia, 2004;Bruford and Wayne, 1993).…”

Section: Analysis Of Genetic Variation In Animalsmentioning

confidence: 99%

“…Dalvit et al, (2009) have used 19 microsatellite markers 5 for genetic variation and presence of breed substructure of four native sheep breeds from North Italy. Arora and Bhatia, (2004) have used 13 microsatellites to asses genetic effects of the population declines in Muzzafarnagri Sheep from India. Tapio et al, (2010) have used 20 microsatellite markers for genetic diversity and population structure of 52 sheep breed from three geographical regions Caucasus, Asia, and the eastern fringe of Europe.…”

Section: Analysis Of Genetic Variation In Animalsmentioning

confidence: 99%