Genetic structure of Korean populations of the clam Ruditapes philippinarum inferred from microsatellite marker analysis

An, Hye Suck; Park, Kwang Jae; Cho, Kee Chae; Han, Hyon‐Sob; Myeong, Jeong‐In

doi:10.1016/j.bse.2012.05.007

Cited by 23 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We detected less genetic diversity than that reported in previous studies on manila clam (An et al, 2012b;Mura et al, 2012). However, our results are similar to those of studies on other species, including Miiuy croaker (Miichthys miiuy; N A = 5.13, Xu et al, 2011), Mediterranean blue mussel (Mytilus galloprovincialis; N A = 3.9, Li et al, 2011), blood clam (T. granosa; N A = 3.9, Dong et al, 2012), and Meretrix meretrix clams (N A = 9.2, Lu et al, 2011).…”

Section: Discussioncontrasting

confidence: 94%

“…Specifically, KRph 9118-1 and KRph 39753-1 displayed a high level of allelic diversity and heterozygosity. A moderate level of allelic diversity was detected in each population (IC, mean N A = 7.20; GJ, mean N A = 7.40), which was dramatically lower than that for many other populations of marine bivalves, including pen shell (mean N A = 20, Liu et al, 2009), small abalone (mean N A = 14.73, Zhan et al, 2009), and manila clam reported from An et al (2012b) (mean N A = 14.17). Overall, the A R varied from 2 to 17 (Table 3).…”

Section: Comparative Genetic Variability Between 2 Manila Clam Populamentioning

confidence: 65%

“…Despite its importance as a highly valuable fishery resource in Korea, R. philippinarum has been the subject of only a few studies (Yasuda et al, 2007;An et al, 2012b). Microsatellite markers have many advantages over other molecular markers for research on population structure and genetic diversity (Guichoux et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, An et al (2012b) reported the genetic relationship of 5 local manila clam populations using 7 microsatellite loci, which suggested 3 genetically distinct populations along the western and southern coasts of Korea. Many previous studies were conducted using allozyme markers (Oniwa et al, 1988;Vargas et al, 2008), mitochondrial DNA (Sekine et al, 2006), amplified fragment length polymorphisms (Liu et al, 2007) and random amplification of polymorphic DNA (Yoon and Kim, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…To date, only 16 genome-derived microsatellite markers are available for R. philippinarum (Yasuda et al, 2007;An et al, 2012b). The statistical power depends not only on the number of scored loci but also on other factors, such as the degree of polymorphism of each locus and the sample size because the use of a limited number of loci might fail to offer sufficient information.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

New polymorphic microsatellite markers for the Korean manila clam (Ruditapes philippinarum) and their application to wild populations

Kim¹,

An²,

Kang³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Manila clam (Ruditapes philippinarum) is a valuableand intensively exploited shellfish species in Korea. Despite its importance, information on its genetic background is scarce. For the genetic characterization of R. philippinarum, expressed sequence tagderived microsatellite markers were developed using next-generation sequencing. A total of 5879 tandem repeats containing di-to hexanucleotide repeat motifs were obtained from 236,746 reads (mean = 413 bp). Of the 62 loci screened, 24 (38.7%) were successfully amplified, and 10 were polymorphic in 144 individuals from 2 manila clam populations (Incheon and Geoje, Korea). The number of alleles ranged from 2 to 17 in the Incheon population and from 3 to 13 in the Geoje population (overall A R = 7.21). The mean observed and expected heterozygosities were estimated to be 0.402 and 0.555, respectively. Hence, there is less genetic variability in the Geoje population than in the Incheon population, although no significant reductions of genetic diversity were found between the populations (P > 0.05). However, significant genetic differentiation was detected between the populations (F ST = 0.064, P < 0.001). Significant deviations from Hardy-Weinberg equilibrium and high inbreeding coefficients (mean F IS = 0.22-0.26) were detected in both populations. The 10 novel polymorphic microsatellite loci used in this study will be useful for future genetic mapping studies and for characterizing population structures, monitoring genetic diversity for successful aquaculture management, and developing conservation strategies for manila clam populations in Korea.

show abstract