Genetic Structure of<i>Aedes aegypti</i>in Australia and Vietnam Revealed by Microsatellite and Exon Primed Intron Crossing Markers Suggests Feasibility of Local Control Options

Endersby, N. M.; Hoffmann, Ary A.; White, Vanessa L.; Lowenstein, Steven M.; Ritchie, Scott A.; Johnson, Petrina H.; Rapley, Luke P.; Ryan, Peter; Nam, Vũ Sinh; Yen, N. T.; Kittiyapong, P.; Weeks, Andrew R.

doi:10.1603/033.046.0514

Cited by 45 publications

(79 citation statements)

References 56 publications

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“…Two EPIC markers (Endersby et al 2009) and six microsatellites (Chambers et al 2007;Slotman et al 2007;Endersby et al 2009) were used to study variation in the backcrosses (see Endersby et al 2009). PCR was performed as a 10-ml reaction per individual.…”

Section: Methodsmentioning

confidence: 99%

“…This consisted of 2 ml of 43 diluted genomic DNA; 0.3 ml [g 33 P]ATP-end-labeled forward primer, 1 mm; 0.1 ml unlabeled forward primer, 10 mm; 0.4 ml reverse primer, 10 mm; 2 ml dNTPs (Bioline), 1 mm; 0.5 ml bovine serum albumin (New England Biolabs, Ipswich, MA), 1%; 1 ml 103 PCR buffer with 2.0 mm Mg 21 ; 0.4 unit of Taq polymerase (New England Biolabs); and 3.62 ml sterile water. PCR specifications are based on previous studies (Chambers et al 2007;Slotman et al 2007;Endersby et al 2009). Thirty females per line were genotyped.…”

Section: Methodsmentioning

confidence: 99%

“…Ae. aegypti populations show isolation by distance, reflecting relatively high levels of genetic isolation in populations (Failloux et al 2002;Huber et al 2002;Endersby et al 2009) likely to generate local adaptation. Competitive strains that capture a locally adapted background can be generated through backcrossing strains to the genetic background of the target population.…”

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confidence: 99%

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Dynamics of the “Popcorn” Wolbachia Infection in Outbred Aedes aegypti Informs Prospects for Mosquito Vector Control

et al. 2011

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Forty percent of the world's population is at risk of contracting dengue virus, which produces dengue fever with a potentially fatal hemorrhagic form. The wMelPop Wolbachia infection of Drosophila melanogaster reduces life span and interferes with viral transmission when introduced into the mosquito Aedes aegypti, the primary vector of dengue virus. Wolbachia has been proposed as an agent for preventing transmission of dengue virus. Population invasion by Wolbachia depends on levels of cytoplasmic incompatibility, fitness effects, and maternal transmission. Here we characterized these traits in an outbred genetic background of a potential target population of Ae. aegypti using two crossing schemes. Cytoplasmic incompatibility was strong in this background, and the maternal transmission rate of Wolbachia was high. The infection substantially reduced longevity of infected adult females, regardless of whether adults came from larvae cultured under high or low levels of nutrition or density. The infection reduced the viability of diapausing and nondiapausing eggs. Viability was particularly low when eggs were laid by older females and when diapausing eggs had been stored for a few weeks. The infection affected mosquito larval development time and adult body size under different larval nutrition levels and densities. The results were used to assess the potential for wMelPop-CLA to invade natural populations of Ae. aegypti and to develop recommendations for the maintenance of fitness in infected mosquitoes that need to compete against field insects.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Dynamics of the “Popcorn” Wolbachia Infection in Outbred Aedes aegypti Informs Prospects for Mosquito Vector Control

et al. 2011

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“…24 Also, because dengue is not endemic, selection pressure on the vector from the virus is most likely rare and episodic rather than constant. A previous study examining vector competence in Australian A. aegypti revealed variation in the trait, although the vast majority of difference was between Torres Strait Island and mainland Australian populations.…”

Section: Introductionmentioning

confidence: 99%

Comparative Susceptibility of Mosquito Populations in North Queensland, Australia to Oral Infection with Dengue Virus

Ye¹,

Ng²,

Frentiu³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Dengue is the most prevalent arthropod-borne virus, with at least 40% of the world's population at risk of infection each year. In Australia, dengue is not endemic, but viremic travelers trigger outbreaks involving hundreds of cases. We compared the susceptibility of Aedes aegypti mosquitoes from two geographically isolated populations to two strains of dengue virus serotype 2. We found, interestingly, that mosquitoes from a city with no history of dengue were more susceptible to virus than mosquitoes from an outbreak-prone region, particularly with respect to one dengue strain. These findings suggest recent evolution of population-based differences in vector competence or different historical origins. Future genomic comparisons of these populations could reveal the genetic basis of vector competence and the relative role of selection and stochastic processes in shaping their differences. Lastly, we show the novel finding of a correlation between midgut dengue titer and titer in tissues colonized after dissemination.

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“…In recent years, the application of high-resolution molecular markers has provided important new insight into the population genetic structure and infestation dynamics of many insect pest species of public health concern (Conn and Mirabello 2007, Fitzpatrick et al 2008, Paupy et al 2008, Endersby et al 2009, Crissman et al 2010, Pé rez de Rosas et al 2011. New molecular tools now make it feasible to not only accurately identify the number of populations actively infesting a building (Pizarro et al 2008, Crissman et al 2010) but also to elucidate dynamics and characteristics essential for understanding infestation patterns and history, e.g., levels of genetic diversity (a measure often associated with population health; Paupy et al 2008, Piccinali et al 2009), temporal stability of populations after pest control efforts (Pé rez de Rosas et al 2007), and the presence or absence of genetic mutations associated with insecticide resistance (Yoon et al 2008, Zhu et al 2010.…”

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confidence: 99%

Molecular Markers Reveal Infestation Dynamics of the Bed Bug (Hemiptera: Cimicidae) Within Apartment Buildings

et al. 2012

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The bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), has experienced an extraordinary global resurgence in recent years, the reasons for which remain poorly understood. Once considered a pest of lower socioeconomic classes, bed bugs are now found extensively across all residential settings, with widespread infestations established in multiapartment buildings. Within such buildings, understanding the population genetic structure and patterns of dispersal may prove critical to the development of effective control strategies. Here, we describe the development of 24 high-resolution microsatellite markers through next generation 454 pyrosequencing and their application to elucidate infestation dynamics within three multistory apartment buildings in the United States. Results reveal contrasting characteristics potentially representative of geographic or locale differences. In Raleigh, NC, an infestation within an apartment building seemed to have started from a single introduction followed by extensive spread. In Jersey City, NJ, two or more introductions followed by spread are evident in two buildings. Populations within single apartments in all buildings were characterized by high levels of relatedness and low levels of diversity, indicative of foundation from small, genetically depauperate propagules. Regardless of the number of unique introductions, genetic data indicate that spread within buildings is extensive, supporting both active and human-mediated dispersal within and between adjacent rooms or apartments spanning multiple floors.

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Genetic Structure ofAedes aegyptiin Australia and Vietnam Revealed by Microsatellite and Exon Primed Intron Crossing Markers Suggests Feasibility of Local Control Options

Cited by 45 publications

References 56 publications

Dynamics of the “Popcorn” Wolbachia Infection in Outbred Aedes aegypti Informs Prospects for Mosquito Vector Control

Dynamics of the “Popcorn” Wolbachia Infection in Outbred Aedes aegypti Informs Prospects for Mosquito Vector Control

Comparative Susceptibility of Mosquito Populations in North Queensland, Australia to Oral Infection with Dengue Virus

Molecular Markers Reveal Infestation Dynamics of the Bed Bug (Hemiptera: Cimicidae) Within Apartment Buildings

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