Genetic structure and phylogenetic relationships of <i>Leptosphaeria maculans</i> and <i>L. biglobosa</i> in Northern regions of Iran

Zamanmirabadi, Ali; Hemmati, Roghayeh; Dolatabadian, Aria; Batley, Jacqueline

doi:10.1080/03235408.2022.2081653

Cited by 2 publications

(4 citation statements)

References 34 publications

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“…The low proportion of L . maculans isolates contributed from Iran to this collection is consistent with other recent studies (Zamanmirabadi et al, 2022).…”

Section: Resultssupporting

confidence: 93%

“…Of the 18 Iranian isolates, only five isolates were L. maculans, with the remaining 13 being either L. biglobosa 'canadensis' or L. biglobosa 'brassicae' (Table S1). The low proportion of L. maculans isolates contributed from Iran to this collection is consistent with other recent studies (Zamanmirabadi et al, 2022).…”

Section: Assembly Of a Set Of Leptosphaeria Strains Representing Curr...supporting

confidence: 92%

“…Whilst these general subpopulations are detected, there are exceptions: with Argentinian isolates grouping more closely with the European set of isolates and Iranian isolates grouping with North American isolates. Previously Argentina imported canola seed from Europe for many years and Iran has imported seed from Canada for the past two decades (Zamanmirabadi et al, 2022), which may have resulted in the similarities detected in the analysis. Furthermore, the population analysis shows that within New Zealand, isolates from Hawke's Bay on the North Island and the remaining isolates collected from the South Island are distinct, suggesting limited movement over a much smaller geographical distance.…”

Section: Discussionmentioning

confidence: 99%

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A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

Van de Wouw,

Scanlan,

Al‐Mamun

et al. 2023

Plant Pathology

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A collection of isolates of the fungi Leptosphaeria maculans and L. biglobosa, which cause blackleg disease on Brassica napus (canola/oilseed rape) and other Brassicaceae species, was assembled to represent the global diversity of these pathogens and a resource for international research. The collection consists of 226 isolates (205 L. maculans and 21 L. biglobosa) from 11 countries. The genomes of all 205 L. maculans isolates were sequenced, and the distribution and identity of avirulence gene alleles were determined based on genotypic information and phenotypic reactions on B. napus lines that hosted specific resistance genes. Whilst the frequencies of some avirulence alleles were consistent across each of the regions, others differed dramatically, potentially reflecting the canola/oilseed rape cultivars grown in those countries. Analyses of the single‐nucleotide polymorphism (SNP) diversity within these L. maculans isolates revealed geographical separation of the populations. This "open access" resource provides a standardized set of isolates that can be used to define the basis for how these fungal pathogens cause disease, and as a tool for discovery of new resistance traits in Brassica species.

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“…The low proportion of L . maculans isolates contributed from Iran to this collection is consistent with other recent studies (Zamanmirabadi et al, 2022).…”

Section: Resultssupporting

confidence: 93%

Section: Assembly Of a Set Of Leptosphaeria Strains Representing Curr...supporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

Van de Wouw,

Scanlan,

Al‐Mamun

et al. 2023

Plant Pathology

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“…isolates were used for initial validation of the new mating-type diagnostics designed in this study. These included two isolates of P. biglobosus ‘brassicae’ (KO8 from Iran; 21WAS8-4 from the UK) and two isolates of P. biglobosus ‘canadensis’ (21WAS1-2 and 21WAS7-1, both from the UK); isolate, species and subclade identities had previously been confirmed based on at least ITS sequences [ 9 , 33 ]. An isolate of the closely related P. dezfulensis (SCUA-Ahm-S41, Iran) [ 10 ] was also tested.…”

Section: Methodsmentioning

confidence: 99%

Indirect Evidence Based on Mating-Type Ratios for the Role of Sexual Reproduction in European and Chinese Populations of Plenodomus biglobosus (Blackleg of Oilseed Rape)

et al. 2022

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Blackleg (Phoma) disease, caused by the ascomycete fungi Plenodomus biglobosus and P. lingam, threatens oilseed rape (OSR; Brassica napus) crops internationally. In many parts of the world, both species co-occur, but in China only P. biglobosus has so far been reported. Plenodomus biglobosus reproduces asexually (pycnidiospores), but also sexually (pseudothecia-yielding ascospores), via a heterothallic mating system requiring MAT1-1 and MAT1-2 genotypes. However, the roles of airborne ascospore inoculum in driving blackleg disease outbreaks in China are less well understood compared to elsewhere in the world. This is despite the very different agronomic cropping practices in parts of China, in which paddy rice and OSR are often grown in rotation; OSR stubble is often submerged under water for long periods potentially affecting pseudothecial development. Here, we indirectly investigate the potential role of sexual reproduction by developing new polymerase chain reaction (PCR) -based mating-type diagnostics for P. biglobosus and subsequently screening an international collection of 59 European and 157 Chinese isolates. Overall, in both Europe and China, P. biglobosus mating types did not deviate from a 1:1 ratio, such as is generally thought to occur under frequency-dependent selection in sexually reproducing pathogen populations. Both mating types were balanced in all the individual European countries tested (Austria, France, Poland, UK). Conversely, in China, mating types were only balanced in the eastern region; in the northern and southwestern regions there were skewed ratios, more typical of predominantly asexual reproduction, towards MAT1-1 and MAT1-2, respectively. The implications of these findings and future research directions for improved understanding of P. biglobosus epidemiology on OSR, particularly in China, are considered.

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Genetic structure and phylogenetic relationships of Leptosphaeria maculans and L. biglobosa in Northern regions of Iran

Cited by 2 publications

References 34 publications

A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

Indirect Evidence Based on Mating-Type Ratios for the Role of Sexual Reproduction in European and Chinese Populations of Plenodomus biglobosus (Blackleg of Oilseed Rape)

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