Genetic structure and historical demography of Malus sieversii in the Yili Valley and the western mountains of the Junggar Basin, Xinjiang, China

Hong-Xiang, Zhang; Zhang, Mingli; Wang, Lina

doi:10.1007/s40333-014-0044-2

Cited by 20 publications

(20 citation statements)

References 23 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2). However, using sequences from the gene LEAFY, we found that population GL did not have the highest genetic diversity (Zhang et al 2015). Still, levels of genetic diversity among the six populations was ordered differently between our study and previous studies.…”

Section: Discussioncontrasting

confidence: 82%

“…These selected microsatellite primers were tagged with a fluorescent dye at the 5¢ end. The PCR amplification program followed the protocols of Zhang et al (2015), except for the SSR primers used. PCR products were checked with 8% non-denaturing polyacrylamide gel electrophoresis, separated and visualized using an ABI 3730 automated sequencer by Sangon Biotech (Shanghai), and read with the software GeneMapper.…”

Section: Nssr Molecular Laboratory Proceduresmentioning

confidence: 99%

“…The conservation genetics, genetic diversity, and relationship among separated populations of M. sieversii have been the focus of several previous studies (Zhang et al 2007, Zhang 2008, Qin et al 2012, Dong et al 2013, Zhang et al 2015. These studies, however, have not clearly delimited priority conservation areas.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identifying evolutionarily significant units for conservation of the endangered Malus sieversii using genome‐wide RADseq data

Zhang

2018

Nordic Journal of Botany

View full text Add to dashboard Cite

Malus sieversii, a wild progenitor of the domesticated apple, is an endangered species and is assigned second conservation priority by the China Plant Red Data Book. It is urgent to carry out in situ conservation of this species, but previous studies have not identified evolutionarily significant units (ESUs) for conservation management. In this study, we investigated the genetic diversity and relationships of six M. sieversii populations from China using integrated analysis of microsatellite (nSSR) data, genome‐wide SNPs and previous results in order to propose a reasonable conservation management. The results showed that levels of genetic diversity were inconsistently reflected by our nSSR and previous studies, suggesting that indices of genetic diversity are not effective to identify priority conservation areas for M. sieversii. Based on the selection criteria of ESUs for endangered species conservation, ESUs should reflect lineage divergence, geographical separation and different adaptive variation. Our phylogenetic tree based on genome‐wide SNPs yielded a clear relationship of divergent lineages among M. sieversii populations, leading to new different from those of previous studies. Three independent lineages, including the pairs of populations Huocheng‐Yining, Gongliu‐Xinyuan and Tuoli‐Emin, were identified. The geographic distances between populations among the different phylogenetic lineages were much greater than those within the same phylogenetic lineage. A cluster analysis on environmental variables showed that the three independent lineages inhabit different environmental conditions, suggesting that they may have adapted to different environments. Based on the results, we propose that three independent ESUs should be recognized as conservation units for M. sieversii in China.

show abstract

Section: Discussioncontrasting

confidence: 82%

Section: Nssr Molecular Laboratory Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

Identifying evolutionarily significant units for conservation of the endangered Malus sieversii using genome‐wide RADseq data

Zhang

2018

Nordic Journal of Botany

View full text Add to dashboard Cite

show abstract

“…Owing to the important role of M. sieversii in apple breeding, this species has been studied in terms of its genetic diversity and population structure Richards et al, 2009), gene flow with Malus domestica (Cornille et al, 2013b), historical demography (Zhang et al, 2015) and molecular basis for abiotic resistance (Forsline et al, 2003). As to the S-alleles controlling cross-compatibility, few studies have investigated them in M. sieversii, however, such alleles have been extensively studied for apple cultivars and other fruit crops within Rosaceae (Broothaerts, 2003;Sanzol, 2009;Long et al, 2010) owing to the importance of SI to fruit production.…”

Section: Introductionmentioning

confidence: 99%

Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple

Cai

Liu

et al. 2017

Heredity

View full text Add to dashboard Cite

The self-incompatibility (SI) gene that is specifically expressed in pistils encodes the SI-associated ribonuclease (S-RNase), functioning as the female-specificity determinant of a gametophytic SI system. Despite extensive surveys in Malus domestica, the S-alleles have not been fully investigated for Malus sieversii, the primary wild ancestor of the domesticated apple. Here we screened the M. sieversii S-alleles via PCR amplification and sequencing, and identified 14 distinct alleles in this species. By contrast, nearly 40 are present in its close wild relative, Malus sylvestris. We further sequenced 8 nuclear genes to provide a neutral reference, and investigated the evolution of S-alleles via genealogical and population genetic analyses. Both shared ancestral polymorphism and an excess of non-synonymous substitution were detected in the S-RNases of the tribe Maleae in Rosaceae, indicating the action of long-term balancing selection. Approximate Bayesian Computations based on the reference neutral loci revealed a severe bottleneck in four of the six studied M. sieversii populations, suggesting that the low number of S-alleles found in this species is mainly the result of diversity loss due to a drastic population contraction. Such a bottleneck may lead to ambiguous footprints of ongoing balancing selection detected at the S-locus. This study not only elucidates the constituents and number of S-alleles in M. sieversii but also illustrates the potential utility of S-allele number shifts in demographic inference for self-incompatible plant species.

show abstract

“…Although wild apple forests occupy an estimated 93% among the wild fruit forests in Chinese Tianshan, compared with 78% for the central Asian portion (Lin & Lin, 2000), the wild apple forests are not well studied. The wild apple M. sieversii is the primary progenitor of all cultivated domesticated apple species (Duan et al, 2017;Harris, Robinson, & Juniper, 2002;Hokanson et al, 1998;Richards et al, 2009;Zhang, Zhang, & Wang, 2015) and therefore is a globally important resource for apple breeding because of the rich genetic diversity. Thus, the ex and in situ preservation of this species is globally important (Hokanson et al, 1998;Yang et al, 2016).…”

mentioning

confidence: 99%

Agrilus maliMatsumara (Coleoptera: Buprestidae), a new invasive pest of wild apple in western China: DNA barcoding and life cycle

Bozorov

Luo

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

Agrilus mali Matsumara (Coleoptera: Buprestidae) is a wood‐boring beetle distributed to eastern China that occasionally injures apple species. However, this wood‐boring beetle is new to the wild apple forests (Malus sieversii) of the Tianshan Mountains (western China) and has caused extensive tree mortality. The development of a biological control program for these wild apple forests is a high priority that requires exploration of the life cycle, DNA barcoding and taxonomic status of A. mali. In this study, to determine the diversity of invasive beetles, a fragment of the mitochondrial cytochrome oxidase gene was analyzed. Based on the results, beetles from Gongliu and Xinyuan counties of Xinjiang were identical but differed from those in the apple nursery of Gongliu by a single‐nucleotide substitution. We summarize the taxonomic status, relationships, and genetic distances of A. mali among other Agrilus species using the Tajima‐Nei model in maximum likelihood phylogeny. Analysis revealed that A. mali was closely related to Agrilus mendax and both belong to the Sinuatiagrulus subgenus. The life cycle of A. mali was investigated based on a monthly regular inspection in the wild apple forests of Tianshan. Similar to congeneric species, hosts are injured by larvae of A. mali feeding on phloem tissue, resulting in serpentine galleries constructed between bark and xylem that prevent nutrient transport and leading to tree mortality. Future studies will focus on plant physiological responses to the invasive beetles and include surveys of natural enemies for a potential classical biological control program.

show abstract

Genetic structure and historical demography of Malus sieversii in the Yili Valley and the western mountains of the Junggar Basin, Xinjiang, China

Cited by 20 publications

References 23 publications

Identifying evolutionarily significant units for conservation of the endangered Malus sieversii using genome‐wide RADseq data

Identifying evolutionarily significant units for conservation of the endangered Malus sieversii using genome‐wide RADseq data

Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple

Agrilus maliMatsumara (Coleoptera: Buprestidae), a new invasive pest of wild apple in western China: DNA barcoding and life cycle

Contact Info

Product

Resources

About