Genetic structure and connectivity among Aedes aegypti populations within Madurai city in Southern India

Kumar, Mukesh; Kalimuthu, M.; Selvam, A. George Maria; Ashokkumar, Manickam; Paramasivan, R; Kumar, Ashwani; Gupta, Bhavna

doi:10.1016/j.meegid.2021.105031

Cited by 5 publications

(8 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, regardless of their country of origin, the vast majority of studies worldwide suggest the existence of two distinct lineages of Ae. aegypti [11,12,16,19,20,24,26,27,30,46,47]. Our results are consistent with the literature.…”

Section: Discussionsupporting

confidence: 92%

“…Studies based on different nuclear or mitochondrial molecular markers applied to mosquitoes collected in the Americas [9][10][11]16,18,40] report lower genetic diversity than in some Asian countries [25,26,30,[41][42][43][44] and most of Africa [22,31,45]. However, regardless of their country of origin, the vast majority of studies worldwide suggest the existence of two distinct lineages of Ae.…”

Section: Discussionmentioning

confidence: 99%

“…Population structure has been demonstrated in mosquitoes from several countries [30][31][32], while genetic homogeneity and gene flow have been described in other populations [10,[33][34][35]. The degree of genetic structure and variability could depend on the success of national vector control campaigns, unplanned urbanization, migratory movements, the flow of international trade from each country, how rapidly mosquito populations expand, the number of colonization events, and the size of the founder populations.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Diversity among Four Populations of Aedes aegypti (Diptera: Culicidae) from Honduras as Revealed by Mitochondrial DNA Cytochrome Oxidase I

et al. 2022

View full text Add to dashboard Cite

Aedes aegypti is a hematophagous and highly anthropophilic mosquito with a wide distribution, particularly in tropical and subtropical regions of the world. Ae. aegypti is the main vector of several febrile diseases called arboviruses (dengue, yellow fever, chikungunya, and zika viruses), which represent an important public health problem. Populations of this mosquito were nearly eliminated from the Americas in the mid-20th century; however, after the abandonment of control measures, mosquito populations have been recovering territory, have expanded by anthropogenic mechanisms, and have been joined by new populations reintroduced from other continents. The objective of this pilot study was to determine the genetic variability of Aedes aegypti collected in four cities located along the so-called logistics corridor of Honduras, which connects the Caribbean Sea to the Pacific Ocean. We studied the sequences of two molecular markers: the cytochrome c oxidase 1 (COI mtDNA) gene and the internal transcribed spacer 2 (ITS2 rDNA) of 40 mosquitoes. Phylogenetic analyzes show two separate clades with a low number of nucleotide differences per site, three haplotypes, and low haplotype diversity. These results suggest a low genetic diversity in the populations of Ae. aegypti in Honduras in relation to that reported in other countries of the Central American isthmus.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Diversity among Four Populations of Aedes aegypti (Diptera: Culicidae) from Honduras as Revealed by Mitochondrial DNA Cytochrome Oxidase I

et al. 2022

View full text Add to dashboard Cite

show abstract

“…GENETIX version 4.05 60 was used to perform FCA based on genotypic data obtained for individuals from the populations. Correction for multiple testing for HWE, LD, FIS and Wilcoxon’s test was performed using the FDR approach as described in Benjamini & Hochberg (1995) at the 95% confidence level.…”

Section: Methodsmentioning

confidence: 99%

Population genetic structure of Aedes aegypti subspecies in selected geographical locations in Sudan

Abuelmaali,

Mashlawi,

Ishak

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Although knowledge of the composition and genetic diversity of disease vectors is important for their management, this is limiting in many instances. In this study, the population structure and phylogenetic relationship of the two Aedes aegypti subspecies namely Aedes aegypti aegypti (Aaa) and Aedes aegypti formosus (Aaf) in eight geographical areas in Sudan were analyzed using seven microsatellite markers. Hardy–Weinberg Equilibrium (HWE) for the two subspecies revealed that Aaa deviated from HWE among the seven microsatellite loci, while Aaf exhibited departure in five loci and no departure in two loci (A10 and M201). The Factorial Correspondence Analysis (FCA) plots revealed that the Aaa populations from Port Sudan, Tokar, and Kassala clustered together (which is consistent with the unrooted phylogenetic tree), Aaf from Fasher and Nyala populations clustered together, and Gezira, Kadugli, and Junaynah populations also clustered together. The Bayesian cluster analysis structured the populations into two groups suggesting two genetically distinct groups (subspecies). Isolation by distance test revealed a moderate to strong significant correlation between geographical distance and genetic variations (p = 0.003, r = 0.391). The migration network created using divMigrate demonstrated that migration and gene exchange between subspecies populations appear to occur based on their geographical proximity. The genetic structure of the Ae. aegypti subspecies population and the gene flow among them, which may be interpreted as the mosquito vector's capacity for dispersal, were revealed in this study. These findings will help in the improvement of dengue epidemiology research including information on the identity of the target vector/subspecies and the arboviruses vector surveillance program.

show abstract

“…Genotyping was performed using 12 microsatellite markers, including four tri-nucleotide repeats (A1, B2, B3, A9) and eight di-nucleotide repeats (AC2, CT2, AG2, AC4, AC1, AC5, AG1, and AG4) [ 24 , 44 ]. Amplification was performed with Qiagen Type-it-microsatellite kit using fluorescently labeled primers as described earlier [ 22 ]. Each forward primer had a M13 tail and two M13 primers each tagged with FAM and HEX, respectively were used to obtain fluorescent labeled amplified products.…”

Section: Materials Methodsmentioning

confidence: 99%

Genetic differentiation among Aedes aegypti populations from different eco-geographical zones of India

Sumitha¹,

Kalimuthu²,

Kumar³

et al. 2023

PLoS Negl Trop Dis

Self Cite

View full text Add to dashboard Cite

The present study explicitly evaluated the genetic structure of Aedes aegypti Linn, the vector of dengue, chikungunya, and Zika viruses, across different geo-climatic zones of India and also elucidated the impact of ecological and topographic factors. After data quality checks and removal of samples with excess null alleles, the final analysis was performed on 589 individual samples using 10 microsatellite markers. Overall findings of this study suggested that, Ae. aegypti populations are highly diverse with moderate genetic differentiation between them. Around half of the populations (13 out of 22) formed two genetic clusters roughly associated with geographical regions. The remaining nine populations shared genetic ancestries with either one or both of the clusters. A significant relationship between genetic and geographic distance was observed, indicating isolation by distance. However, spatial autocorrelation analysis predicted the signs of long-distance admixture. Post-hoc environmental association analysis showed that 52.7% of genetic variations were explained by a combination of climatic and topographic factors, with latitude and temperature being the best predictors. This study indicated that though overall genetic differentiation among Ae. aegypti populations across India is moderate (Fst = 0.099), the differences between the populations are developing due to the factors associated with geographic locations. This study improves the understanding of the Ae. aegypti population structure in India that may assist in predicting mosquito movements across the geo-climatic zones, enabling effective control strategies and assessing the risk of disease transmission.

show abstract

Genetic structure and connectivity among Aedes aegypti populations within Madurai city in Southern India

Cited by 5 publications

References 60 publications

Genetic Diversity among Four Populations of Aedes aegypti (Diptera: Culicidae) from Honduras as Revealed by Mitochondrial DNA Cytochrome Oxidase I

Genetic Diversity among Four Populations of Aedes aegypti (Diptera: Culicidae) from Honduras as Revealed by Mitochondrial DNA Cytochrome Oxidase I

Population genetic structure of Aedes aegypti subspecies in selected geographical locations in Sudan

Genetic differentiation among Aedes aegypti populations from different eco-geographical zones of India

Contact Info

Product

Resources

About