Genetic stability of microspore-derived doubled haploids of barley: a cytological, biochemical, and molecular study

Abstract

W. 1991. Genetic stability of microspore-derived doubled haploids of barley: a cytological, biochemical, and molecular study. Genome, 34: 923-928. Populations of doubled haploids were produced by culturing microspores from spring barley cv. Tweed, cv. Tyne, and cv. Natasha, on media containing either sucrose or maltose as the only carbohydrate. The populations were analysed for evidence of genetic instability at the karyotype, protein, and DNA levels. The results show very little evidence of induced genetic change. The level of instability is insignificant when compared with that which is observed following meiotic recombination. The stability of microspore-derived lines may be attributed to the embryogenic mode of regeneration, which occurs at a high frequency on a maltose-containing medium. The results are discussed in relation to the exploitation of doubled haploids in barley breeding. et POWELL, W. 1991. Genetic stability of microspore-derived doubled haploids of barley: a cytological, biochemical, and molecular study. Genome, 34 : 923-928. Des populations d'haploi'des doublees ont ete produites par culture d'androspores d'orge de printemps : cv. Tweed, cv. Tyne et cv. Natasha, sur des milieux contenant soit du sucrose ou du maltose comme seule source d'hydrate de carbone. Les populations ont ete analysees pour les evidences d'instabilite genetique aux niveaux du caryotype, des proteines et de I'ADN. Les resultats montrent trks peu d'evidences de changements induits. Le niveau d'instabilite n'est pas significatif, lorsqu'il est compare a celui qui est observe au cours de la recombinaison meiotique. La stabilite des lignees derivees d'androspores peut 2tre attribuee au mode embryogenique de la regeneration qui survient a une frequence elevee, sur un milieu contenant du maltose. Les resultats sont discutes en regard de l'exploitation des haploi'des doublees dans les programmes d'amelioration de l'orge.

Comparisons between doubled haploid lines produced by anther culture, the Hordeum bulbosum-method and lines produced by single seed descent in barley crosses

“…It cannot be ruled out that such effects may affect our results. In addition we have found that the frequency of spontaneous doubling (haploids/diploid ratio) in AC differed among the 3 crosses (results not shown), in agreement with the observations by Finnie et al (1991). Obviously, the assumption (3) of no gametic selection in AC cannot be tested on this background.…”

“…They also observed differences between spontaneously doubled or colchicine treated ACs. Finnie et al (1991) examined karyotype, protein and DNA instability in ACs from 3 pure line cultivars (a 'within line' approach). In a sample of 310 ACs produced on a maltose medium and 20 on a sucrose medium they found no traces of such instability.…”

Pathways to doubled haploidy: chromosome doubling during androgenesis

“…In this regard, there are several studies indicating that genetic instability contributed by the methods of DH production is negligible (Baenziger et al, 1991;Finnie et al, 1991). In maize, somaclonal variation produced by somatic callus-derived plants and androgenic plants was recently compared (Barret et al, 2006).…”

Evidence for gametoclonal variation in potato (Solanum tuberosum L.)

“…High genetic stability and apparent lack of gametoclonal variation have been observed in anther-derived progenies of diploid (2n = 2x = 24) potato clones (Meyer et al 1993) and wild species, such as S. chacoense (Rivard et al 1994) and S. chacoense 9 S. phureja (Veilleux et al 1995). This apparent lack of gametoclonal variation has also been reported in microspore-derived doubled haploids of barley (Finnie et al 1991;Munoz et al 2004) and maize (Bentolila et al 1992), and in a gynogenic population of onion (Javornik et al 1998).…”