Genetic stability analysis using DNA barcoding and molecular markers and foliar micro-morphological analysis of in vitro regenerated and in vivo grown plants of Artemisia vulgaris L.

Jogam, Phanikanth; Sandhya, Dulam; Shekhawat, Mahipal S.; Alok, Anshu; Manokari, M.; Abbagani, Sadanandam; Allini, Venkateswar Rao

doi:10.1016/j.indcrop.2020.112476

Cited by 45 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main aim of in vitro propagation techniques is to obtain true-to-type plantlets without genetic variations compared to the mother plants [ 47 ]. Somaclonal variations are generally introduced among the regenerants during the process of in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

High efficient de novo root-to-shoot organogenesis in Citrus jambhiri Lush.: Gene expression, genetic stability and virus indexing

et al. 2021

View full text Add to dashboard Cite

A protocol for high-frequency direct organogenesis from root explants of Kachai lemon (Citrus jambhiri Lush.) was developed. Full-length roots (~3 cm) were isolated from the in vitro grown seedlings and cultured on Murashige and Skoog basal medium supplemented with Nitsch vitamin (MSN) with different concentrations of cytokinin [6-benzylaminopurine, (BAP)] and gibberellic acid (GA3). The frequency of multiple shoot proliferation was very high, with an average of 34.3 shoots per root explant when inoculated on the MSN medium supplemented with BAP (1.0 mg L–1) and GA3 (1.0 mg L–1). Optimal rooting was induced in the plantlets under half strength MSN medium supplemented with indole-3-acetic acid (IAA, 0.5–1.0 mg L–1). IAA induced better root structure than 1-naphthaleneacetic acid (NAA), which was evident from the scanning electron microscopy (SEM). The expressions of growth regulating factor genes (GRF1 and GRF5) and GA3 signaling genes (GA2OX1 and KO1) were elevated in the regenerants obtained from MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1). The expressions of auxin regulating genes were high in roots obtained in ½ MSN+IAA 1.0 mg L-1. Furthermore, indexing of the regenerants confirmed that there was no amplicons detected for Huanglongbing bacterium and Citrus tristeza virus. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers detected no polymorphic bands amongst the regenerated plants. This is the first report that describes direct organogenesis from the root explant of Citrus jambhiri Lush. The high-frequency direct regeneration protocol in the present study provides an enormous significance in Citrus organogenesis, its commercial cultivation and genetic conservation.

show abstract

Section: Discussionmentioning

confidence: 99%

High efficient de novo root-to-shoot organogenesis in Citrus jambhiri Lush.: Gene expression, genetic stability and virus indexing

et al. 2021

View full text Add to dashboard Cite

show abstract

“…These results, as per SCoT, ISSR, and RAPD analyses, suggest that there is apparent variation within the species Corynandra chelidonii. The SCoT, RAPD, and ISSR markers were demonstrated to be not only effective techniques to screen genetic differences but also quick, very consistent, cost-effective and one can investigate the whole genome of the plant randomly (Jogam et al 2020;Pendli et al 2019;Rohela et al 2020). The use of more than one molecular marker to detect the genetic dissimilarity is always useful for better results because every molecular marker has its benefits and drawbacks.…”

Section: Relative Differentiation and Estimate Of Gene Flowmentioning

confidence: 99%

Intraspecific genetic variation inCorynandra chelidonii(Angiosperms: Cleomaceae) as revealed by SCoT, ISSR and RAPD analyses

et al. 2020

Self Cite

View full text Add to dashboard Cite

The genetic diversity of two subpopulations of Corynandra chelidonii, one of terrestrial and the other of aquatic environments, was measured with molecular markers, such as start codon targeted (SCoT), inter simple sequence repeats (ISSR), and random amplification of polymorphic DNA (RAPD). The traditional morphological traits such as habitat, habit, leaf morphology, the colour of the sepals and petals, number of stamens, and seed morphology formed the base for their realization as two varieties, C. chelidonii var. pallae and C. chelidonii var. chelidonii. The polymorphism between the two variants was 100% with the primers SCoT-2 and OPA-1 and 4, while maximum polymorphism was detected with ISSR-2, SCoT-3, and OPA-3. The study used, for the first time, more than one molecular marker to assess the genetic variation underscoring the morphological variation in Corynandra chelidonii (L.f.) Cochrane & Iltis. The study justifies the recognition of the two subpopulations of Corynandra chelidonii from aquatic and terrestrial environments as two distinct varieties, C. chelidonii var. pallae (Reddy & Raju) V.S.Raju and C. chelidonii var. chelidonii, respectively, based on the traditional taxonomic evidence.

show abstract

“…DC is an important medicinal and aromatic shrub which belongs to the family Asteraceae and found mostly in south India (Pala et al 2016). Several medicinally important Artemisia species are distributed throughout various parts of Asia, Europe, the Middle East, and North Africa (Nigam et al 2019;Jogam et al 2020). It is commonly known as "Davana" and shows several important medicinal properties such as anti-in ammatory, anti-pyretic, anti-microbial, antioxidant, antidiabetic, antihelmintic, anti-malarial, antiseptic, antihypertensive, antidepressant, balsamic, choleretic, digestive, depurative, diuretic, emmenagogue, insecticidal, and also used in the treatment of leukemia, wound healing, and some skin infections especially sclerosis (Suresh et al 2011;Haider et al 2014;Shreyas et al 2018;Hiremath et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-Mediated Genetic Transformation and Cloning of Reference Genes in Suspension Cells of Artemisia Pallens

Jogam

Sandhya

Alok

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

A reliable and stable Agrobacterium-mediated genetic transformation system has been developed using cell suspension cultures derived from Artemisia pallens cotyledon explants. Cotyledon, attached cotyledon, and compound leaf were found to be suitable for the induction of callus among five different types of explants tested. Yellow friable callus derived from attached cotyledon was used to initiate suspension cultures in Suspension Culture Medium (SCM) which was supplemented with 2.4-dichlorophenoxyacetic acid (2,4-D) and in combination with different concentrations of Zeatin (ZEA). Among the two different shock treatments, cold shock (at 4 oC) for 20 minutes and heat shock (at 45oC) treatment for 5 minutes, heat shock treatment increased the transformation efficiency. Supplementation of chemical additives such as Silwet L-77 (0.05%) and Pluronic F-68 (0.05%) significantly enhanced suspension cultures' transformation efficiency. The maximum GUS intensity was recorded with an optimal intensity of blue spots in the transformed cells. The highest GUS fluorometric activity was measured as 879.4±113.7 nmol 4MU/mg/min in transformed cell suspension cultures. The hygromycin-resistant callus derived from micro-calli showed intense blue colour in GUS histochemical assay. The transgene integration into the plant genome was confirmed by polymerase chain reaction (PCR) using uidA specific primers in six hygromycin-resistant cell lines. The cloned and mRNA expression levels of three candidate reference genes ADP-ribosylation factor (Arf), β-actin (Act), and ubiquitin (Ubi), and carotenoid biosynthesis pathway gene, i.e., Phytoene desaturase (Pds) along with transgene (uidA) were evaluated in transgenic callus lines. The present Agrobacterium-mediated genetic transformation protocol could help in better understand the metabolic pathways of this medicinally important plant and its genetic improvement.

show abstract

Genetic stability analysis using DNA barcoding and molecular markers and foliar micro-morphological analysis of in vitro regenerated and in vivo grown plants of Artemisia vulgaris L.

Cited by 45 publications

References 47 publications

High efficient de novo root-to-shoot organogenesis in Citrus jambhiri Lush.: Gene expression, genetic stability and virus indexing

High efficient de novo root-to-shoot organogenesis in Citrus jambhiri Lush.: Gene expression, genetic stability and virus indexing

Intraspecific genetic variation inCorynandra chelidonii(Angiosperms: Cleomaceae) as revealed by SCoT, ISSR and RAPD analyses

Agrobacterium-Mediated Genetic Transformation and Cloning of Reference Genes in Suspension Cells of Artemisia Pallens

Contact Info

Product

Resources

About