Genetic similarity between coriander genotypes using ISSR markers

Abstract

With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16), resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander.

Chemical composition and antimicrobial activity of the essential oils in different populations of Coriandrum sativum L. (coriander) from Iran and Iraq

“…There was significant variation observed among the Iranian and Iraqi populations, and in some cases, populations from each country did not group together closely. Similar results have been reported in previous studies that investigated genetic molecular (Melo et al, 2011), morphological, and agricultural variables (Awas et al, 2016;Choudhary et al, 2022;Li et al, 2023).…”

Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits

“…To initiate a breeding program, it is essential to know the genetic variability within the population (Melo et al, 2011). The genetic diversity evaluation among germplasm accessions results in information from potential parents to be used in breeding programs, allowing the duplicate identification and the exchange of germplasm among researchers (Martins et al, 2012).…”

Exchange of germoplasm and genetic diversity to enrichment the Desmanthus genebank

“…However, only after the 2000's they have been studied as species with economic potential. Researches on their genetic diversity are also recent (MELO et al, 2011). Molecular tools can be efficient for identification of superior genotypes, making it possible the concentration of efforts for combinations of promising individuals.…”