Genetic Resources of Cucumber

Naegele, Rachel P.; Wehner, Todd C.

doi:10.1007/7397_2016_15

Cited by 19 publications

(12 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wild cucumber (C. sativus var. hardwickii) still occurs in northern India as well as southern China (Naegele and Wehner, 2016). During domestication, cucumber went through several genetic bottlenecks, causing a strong reduction in genetic diversity, likely due to a small initial population size, combined with a very strong selection pressure, e.g., for absence of bitterness and presence of longer fruit (Qi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Analysis of QTL DM4.1 for Downy Mildew Resistance in Cucumber Reveals Multiple subQTL: A Novel RLK as Candidate Gene for the Most Important subQTL

Berg

Hermans²,

Beenders³

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

One of the biggest problems in cucumber cultivation is cucurbit downy mildew (DM), caused by the obligate biotroph Pseudoperonospora cubensis. Whereas DM in cucumber was previously efficiently controlled by the dm-1 gene from Indian cucumber accession PI 197087, this resistance was broken by new DM strains, prompting the search for novel sources of resistance. A promising source of resistance is the wild cucumber accession PI 197088. It was previously shown that DM resistance in this genotype inherits polygenically. In this paper, we put the focus on one of the QTL, DM4.1 that is located on chromosome 4. QTL DM4.1 was shown to consist of three subQTL: DM4.1.1 affected pathogen-induced necrosis, DM4.1.2 was shown to have an additive effect on sporulation, and DM4.1.3 had a recessive effect on chlorosis as well as an effect on sporulation. Near-isogenic lines (NILs) were produced by introgressing the subQTLs into a susceptible cucumber line (HS279) with good horticultural traits. Transcriptomic analysis revealed that many genes in general, and defense pathway genes in particular, were differentially expressed in NIL DM4.1.1/.2 compared to NIL DM4.1.3 and the susceptible parent HS279. This indicates that the resistance from subQTL DM4.1.1 and/or subQTL DM4.1.2 likely involves defense signaling pathways, whereas resistance due to subQTL DM4.1.3 is more likely to be independent of known defense pathways. Based on fine-mapping data, we identified the RLK gene CsLRK10L2 as a likely candidate for subQTL DM4.1.2, as this gene was found to have a loss-of-function mutation in the susceptible parent HS279, and was strongly upregulated by P. cubensis inoculation in NIL DM4.1.1/.2. Heterologous expression of this gene triggered necrosis, providing further evidence that this gene is indeed causal for subQTL DM4.1.2.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of QTL DM4.1 for Downy Mildew Resistance in Cucumber Reveals Multiple subQTL: A Novel RLK as Candidate Gene for the Most Important subQTL

Berg

Hermans²,

Beenders³

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…The EH, a general statistic for evaluating genetic variation within a population [ 37 ], was higher in Asian and KF1s (0.3290) than in American (0.2225) and European (0.2206) populations and can be explained in relation to the origin of cucumbers. Cucumbers originated in India [ 1 ] and were domesticated in China and Western Asia and then introduced to other regions [ 7 ]. Korean and Japanese cucumber cultivars are believed to have been introduced from China, which is geographically close, and subsequently differentiated into cultivars and accessions with various traits according to the consumers’ preferences and cultivation environment.…”

Section: Discussionmentioning

confidence: 99%

“…It has two primary varieties: cultivated cucumber ( C. sativus var sativus ) and its wild relative ( C. sativus var. hardwickii ) [ 1 ]. Cucumber is a diploid (2n = 2x = 14) with a small genome size of approximately 367 Mbp [ 2 ], and it is recognized as a genetically useful model plant species in terms of a fast generation cycle [ 3 ] and diverse sex expression patterns [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Assessment and Cultivar Identification of Cucumber (Cucumis sativus L.) Using the Fluidigm Single Nucleotide Polymorphism Assay

Park

Choi

Jung³

et al. 2021

Plants

View full text Add to dashboard Cite

Genetic diversity analysis and cultivar identification were performed using a core set of single nucleotide polymorphisms (SNPs) in cucumber (Cucumis sativus L.). For the genetic diversity study, 280 cucumber accessions collected from four continents (Asia, Europe, America, and Africa) by the National Agrobiodiversity Center of the Rural Development Administration in South Korea and 20 Korean commercial F1 hybrids were genotyped using 151 Fluidigm SNP assay sets. The heterozygosity of the SNP loci per accession ranged from 4.76 to 82.76%, with an average of 32.1%. Population genetics analysis was performed using population structure analysis and hierarchical clustering (HC), which indicated that these accessions were classified mainly into four subpopulations or clusters according to their geographical origins. The subpopulations for Asian and European accessions were clearly distinguished from each other (FST value = 0.47), while the subpopulations for Korean F1 hybrids and Asian accessions were closely related (FST = 0.34). The highest differentiation was observed between American and European accessions (FST = 0.41). Nei’s genetic distance among the 280 accessions was 0.414 on average. In addition, 95 commercial F1 hybrids of three cultivar groups (Baekdadagi-, Gasi-, and Nakhap-types) were genotyped using 82 Fluidigm SNP assay sets for cultivar identification. These 82 SNPs differentiated all cultivars, except seven. The heterozygosity of the SNP loci per cultivar ranged from 12.20 to 69.14%, with an average of 34.2%. Principal component analysis and HC demonstrated that most cultivars were clustered based on their cultivar groups. The Baekdadagi- and Gasi-types were clearly distinguished, while the Nakhap-type was closely related to the Baekdadagi-type. Our results obtained using core Fluidigm SNP assay sets provide useful information for germplasm assessment and cultivar identification, which are essential for breeding and intellectual right protection in cucumber.

show abstract

“…Other economically important diseases worldwide include anthracnose (Colletotrichum lagenarium), Fusarium wilt (Fusarium oxysporum f. sp. cucumerinum), gummy stem blight (Didymella bryoniae), scab (Cladosporium cucumerinum), target leaf spot (Corynespora cassiicola), and viruses including Cucumber mosaic virus (CMV) and several potyviruses [51]. Changing production systems may also pose new challenges.…”

Section: Cucumber Vulnerabilitiesmentioning

confidence: 99%

Genetic Resources and Vulnerabilities of Major Cucurbit Crops

Grumet

McCreight

McGregor

et al. 2021

Genes

View full text Add to dashboard Cite

The Cucurbitaceae family provides numerous important crops including watermelons (Citrullus lanatus), melons (Cucumis melo), cucumbers (Cucumis sativus), and pumpkins and squashes (Cucurbita spp.). Centers of domestication in Africa, Asia, and the Americas were followed by distribution throughout the world and the evolution of secondary centers of diversity. Each of these crops is challenged by multiple fungal, oomycete, bacterial, and viral diseases and insects that vector disease and cause feeding damage. Cultivated varieties are constrained by market demands, the necessity for climatic adaptations, domestication bottlenecks, and in most cases, limited capacity for interspecific hybridization, creating narrow genetic bases for crop improvement. This analysis of crop vulnerabilities examines the four major cucurbit crops, their uses, challenges, and genetic resources. ex situ germplasm banks, the primary strategy to preserve genetic diversity, have been extensively utilized by cucurbit breeders, especially for resistances to biotic and abiotic stresses. Recent genomic efforts have documented genetic diversity, population structure, and genetic relationships among accessions within collections. Collection size and accessibility are impacted by historical collections, current ability to collect, and ability to store and maintain collections. The biology of cucurbits, with insect-pollinated, outcrossing plants, and large, spreading vines, pose additional challenges for regeneration and maintenance. Our ability to address ongoing and future cucurbit crop vulnerabilities will require a combination of investment, agricultural, and conservation policies, and technological advances to facilitate collection, preservation, and access to critical Cucurbitaceae diversity.

show abstract

Genetic Resources of Cucumber

Cited by 19 publications

References 110 publications

Analysis of QTL DM4.1 for Downy Mildew Resistance in Cucumber Reveals Multiple subQTL: A Novel RLK as Candidate Gene for the Most Important subQTL

Analysis of QTL DM4.1 for Downy Mildew Resistance in Cucumber Reveals Multiple subQTL: A Novel RLK as Candidate Gene for the Most Important subQTL

Genetic Diversity Assessment and Cultivar Identification of Cucumber (Cucumis sativus L.) Using the Fluidigm Single Nucleotide Polymorphism Assay

Genetic Resources and Vulnerabilities of Major Cucurbit Crops

Contact Info

Product

Resources

About