Genetic Research on Fruit Color Traits of the Bitter Gourd (&lt;i&gt;Momordica charantia&lt;/i&gt; L.)

Huang, Hsiang-Yi; Hsieh, Chi-Ying

doi:10.2503/hortj.mi-159

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…Several studies have shown that the green fruit color is monogenically dominant over the white fruit color ( Srivastava and Nath, 1972 ; Hu et al, 2002 ; Dalamu et al, 2012 ). However, a more recent study indicated that this trait is controlled by quantitative genes ( Huang and Hsieh, 2017 ). Bitter gourd fruit has either a smooth or a distinct warty exterior, and has broken or continuous ridges.…”

Section: Introductionmentioning

confidence: 99%

A RAD-Based Genetic Map for Anchoring Scaffold Sequences and Identifying QTLs in Bitter Gourd (Momordica charantia)

Cui

Luo

et al. 2018

Front. Plant Sci.

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Genetic mapping is a basic tool necessary for anchoring assembled scaffold sequences and for identifying QTLs controlling important traits. Though bitter gourd (Momordica charantia) is both consumed and used as a medicinal, research on its genomics and genetic mapping is severely limited. Here, we report the construction of a restriction site associated DNA (RAD)-based genetic map for bitter gourd using an F2 mapping population comprising 423 individuals derived from two cultivated inbred lines, the gynoecious line ‘K44’ and the monoecious line ‘Dali-11.’ This map comprised 1,009 SNP markers and spanned a total genetic distance of 2,203.95 cM across the 11 linkage groups. It anchored a total of 113 assembled scaffolds that covered about 251.32 Mb (85.48%) of the 294.01 Mb assembled genome. In addition, three horticulturally important traits including sex expression, fruit epidermal structure, and immature fruit color were evaluated using a combination of qualitative and quantitative data. As a result, we identified three QTL/gene loci responsible for these traits in three environments. The QTL/gene gy/fffn/ffn, controlling sex expression involved in gynoecy, first female flower node, and female flower number was detected in the reported region. Particularly, two QTLs/genes, Fwa/Wr and w, were found to be responsible for fruit epidermal structure and white immature fruit color, respectively. This RAD-based genetic map promotes the assembly of the bitter gourd genome and the identified genetic loci will accelerate the cloning of relevant genes in the future.

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Section: Introductionmentioning

confidence: 99%

A RAD-Based Genetic Map for Anchoring Scaffold Sequences and Identifying QTLs in Bitter Gourd (Momordica charantia)

Cui

Luo

et al. 2018

Front. Plant Sci.

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“…There are many studies carried on pericarp color in horticultural plants. The genetic model of fruit color in bitter gourd was found to be a quantitative model through generation mean analysis; green is the dominant trait to white [ 2 ]. Gene mapping of pericarp color in wax gourd showed that the trait was controlled by one single gene; dark green color was dominant over yellow color, and the locus was located on chromosome 5 [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping and Candidate Gene Prediction for White Immature Fruit Skin in Cucumber (Cucumis sativus L.)

Tang

Wang³

et al. 2018

IJMS

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In this study, a single recessive gene (designated w0) was identified to control the white immature fruit color. Genetic mapping with simple sequence repeats (SSR) markers located the w0 gene in the distal region of cucumber chromosome 3 (Chr.3). Fine mapping was then conducted using the method of draft genome scaffold-assisted chromosome walking with 7304 F2 individuals, which allowed for the assignment of the gene locus to a 100.3 kb genomic DNA region with two flanking markers, Q138 and Q193. Thirteen candidate genes were predicted in the 100.3 kb region. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression of the Csa3G904140 gene, which encodes a two-component response regulator-like protein, was much higher in the immature fruit skin of the green parental line (Q1) than in the white parental line (H4). A coding sequence analysis suggested that a single-base insertion occurred at the ninth exon, resulting in a frameshift mutation in Csa3G904140 of H4, and the mutation was consistent with the phenotype in 17 green/white germplasms. Therefore, Csa3G904140 was taken as the likely candidate gene controlling the immature fruit color of cultivated cucumber. This study will contribute to the cloning of candidate genes and the development of white cucumber cultivars using marker-assisted breeding.

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“…The results confirmed earlier report of Hu et al (2002) who explained fruit colour was controlled by one pair of nuclear gene with white recessive and green dominant in nature. In contrary, immature fruit colour in bitter gourd shows continuous distribution from white to dark green pointing this trait was controlled by quantitative genes (Huang and Hsieh 2017). The inheritance of seed coat colour was studied in cross DBGS-54 (Black seed) × DBGS-2 (Brown seed), all the progenies in F 1 bore light black seeds (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Genetic analysis for yield and its attributes in bitter gourd (Momordica charantia)

et al. 2021

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Genetic interaction and inheritance study in bitter gourd was carried out using six-generation mean analysis to determinate the types and magnitude of gene effects for yield and its attributes. The knowledge portioning to gene action and interaction, is tool for designing appropriate breeding strategy for developing varieties in bitter gourd. Four crosses, viz. DBGS-54 × DBGS-34, DBGS-2 × DBGS-34, PVGy-201 × Pusa Do Mousami and DBGS-2 × DBGS-3 were used for studying six generation (P1, P2, F1, F2, B1 and B2) model. The presence of non-allelic interaction were detected by both scaling test and joint scaling test and observed inadequacy of the additive-dominance model for all traits except vine length in cross PVGy-201 × Pusa Do Mousami.The results revealed the contribution of additive (d), dominance (h) and epistasis interaction (i, j, l) towards expression of all traits studied. In most of the characters magnitude of dominance gene effect was greater than additive gene effect suggested that recurrent selection or heterosis breeding would prove useful. Duplicate epistasis was prevalent in most of the traits like vine length, days to first female flower and fruit weight. Fruit epicarp colour and seed coat colour inheritance suggested these traits were controlled by monogenic.

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Genetic Research on Fruit Color Traits of the Bitter Gourd (<i>Momordica charantia</i> L.)

Cited by 12 publications

References 15 publications

A RAD-Based Genetic Map for Anchoring Scaffold Sequences and Identifying QTLs in Bitter Gourd (Momordica charantia)

A RAD-Based Genetic Map for Anchoring Scaffold Sequences and Identifying QTLs in Bitter Gourd (Momordica charantia)

Fine Mapping and Candidate Gene Prediction for White Immature Fruit Skin in Cucumber (Cucumis sativus L.)

Genetic analysis for yield and its attributes in bitter gourd (Momordica charantia)

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