Genetic requirements for uropathogenicE. coliproliferation in the bladder cell infection cycle
Daniel G. Mediati,
Tamika A. Blair,
Ariana Costas
et al.
Abstract:UropathogenicEscherichia coli(UPEC) requires an adaptable physiology to survive the wide range of environments experienced in the host, including gut and urinary tract surfaces. To identify UPEC genes required during intracellular infection, we developed a transposon-directed insertion-site sequencing (TraDIS) approach for cellular infection models and searched for genes in a library of ∼20,000E. coliUTI89 transposon-insertion mutants that are specifically required for growth in M9-glycerol minimal medium, and… Show more
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