Genetic requirements for initiating asexual development in Aspergillus nidulans

Wieser, Jenny; Lee, Bee Na; Fondon, John W.; Adams, Tom

doi:10.1007/bf00326580

Cited by 164 publications

(194 citation statements)

References 24 publications

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“…The results obtained in this work lead us to propose that KapI is not the importer of FlbB. In addition, as brlA is expressed in the null DkapI background but not in flbD null (Wieser & Adams, 1995) or flbC mutants (Wieser et al, 1994), we discard the possibility of an exclusive import of those two TFs by KapI.…”

Section: Discussionmentioning

confidence: 65%

“…(G g ) and at the periphery of the colony (G p ), and specific growth rate (m) of wild-type and DkapI strains All parameters were calculated as described in Methods and are means±SEM of multiple measurements as described in Results. brlA is expressed in the absence of KapI activity UDA factors are positively required for brlA transcription (see Introduction) and some of the mutants in this regulatory pathway are called flb due to the fluffy phenotype associated with a low brlA expression level (Wieser et al, 1994). In contrast to DflbB (Etxebeste et al, 2008), or to other flb mutants such as DflbE , we detected brlA transcription in the DkapI deletion strain (Fig.…”

Section: Flbb Nuclear Import Does Not Require Kapimentioning

confidence: 81%

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KapI, a non-essential member of the Pse1p/Imp5 karyopherin family, controls colonial and asexual development in Aspergillus nidulans

Etxebeste

Markina-Iñarrairaegui

Garzia

et al. 2009

Microbiology

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Asexual development in the filamentous fungus Aspergillus nidulans is governed by the timely expression and cellular localization of multiple transcription factors. Hence, factors mediating import and export across the nuclear pore complexes (karyopherins) are expected to play a key role in coordinating the developmental programme. Here we characterize KapI, a putative homologue of the Saccharomyces cerevisiae Kap121/Pse1p karyopherin. KapI is a non-essential importin-b-like protein located in the nucleus during vegetative growth and conidiophore development. The DkapI phenotype is aconidial with many aerial hyphae. This phenotype can be suppressed under abiotic stress. In this regard, it resembles that of the null allele of the bZIP transcription factor FlbB. However a DflbB; DkapI double mutant exhibited an additive phenotype with totally impaired conidiation, unresponsive to abiotic stress. In contrast to DflbB, the null kapI mutant is not a fluffy-low-bristle expression mutant. Taken together the findings indicate that KapI is required during asexual development, mediating the nuclear transport of factors acting in a different pathway(s) from those involving the upstream developmental activators.

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Section: Discussionmentioning

confidence: 65%

Section: Flbb Nuclear Import Does Not Require Kapimentioning

confidence: 81%

KapI, a non-essential member of the Pse1p/Imp5 karyopherin family, controls colonial and asexual development in Aspergillus nidulans

Etxebeste

Markina-Iñarrairaegui

Garzia

et al. 2009

Microbiology

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“…Further studies by Kale et al (60,61) revealed that these A. flavus and A. parasiticus developmental mutants also lack aflR and aflatoxin enzymatic gene transcripts and fail to accumulate other aflatoxin pathway intermediates. A similar phenotype was found in developmental A. nidulans mutants called "fluffy" (37,78,117,123). Hicks et al (55) demonstrated that some of these A. nidulans developmental mutants are also deficient in sterigmatocystin production.…”

Section: Signal Transduction Pathwaymentioning

confidence: 59%

“…From these examples it is clear that the G-protein signaling pathway is essential for fungal growth and infection. (4,5,37,70,123). Two of these genes, fluG and flbA, were later found to regulate both asexual development and sterigmatocystin production (55), with fluG believed to act upstream of flbA (Fig.…”

Section: Overview Of Signal Transduction In Fungimentioning

confidence: 99%

Relationship between Secondary Metabolism and Fungal Development

Calvo

Wilson

Bok

et al. 2002

Microbiol Mol Biol Rev

932

652

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Filamentous fungi are unique organisms—rivaled only by actinomycetes and plants—in producing a wide range of natural products called secondary metabolites. These compounds are very diverse in structure and perform functions that are not always known. However, most secondary metabolites are produced after the fungus has completed its initial growth phase and is beginning a stage of development represented by the formation of spores. In this review, we describe secondary metabolites produced by fungi that act as sporogenic factors to influence fungal development, are required for spore viability, or are produced at a time in the life cycle that coincides with development. We describe environmental and genetic factors that can influence the production of secondary metabolites. In the case of the filamentous fungus Aspergillus nidulans, we review the only described work that genetically links the sporulation of this fungus to the production of the mycotoxin sterigmatocystin through a shared G-protein signaling pathway

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“…The genes fluG and flbA-C encode developmental regulators that act upstream of brlA, and their deletion reduces the expression of brlA, resulting in aconidial, fluffy phenotypes (Adams et al 1992(Adams et al , 1998Wieser et al 1994). Thus, our next question was whether these regulatory genes were also induced by light.…”

Section: Identification Of Light-regulated Conidiation Genes In a Nimentioning

confidence: 99%

Regulation of Conidiation by Light in Aspergillus nidulans

et al. 2011

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Light regulates several aspects of the biology of many organisms, including the balance between asexual and sexual development in some fungi. To understand how light regulates fungal development at the molecular level we have used Aspergillus nidulans as a model. We have performed a genome-wide expression analysis that has allowed us to identify .400 genes upregulated and .100 genes downregulated by light in developmentally competent mycelium. Among the upregulated genes were genes required for the regulation of asexual development, one of the major biological responses to light in A. nidulans, which is a pathway controlled by the master regulatory gene brlA. The expression of brlA, like conidiation, is induced by light. A detailed analysis of brlA light regulation revealed increased expression after short exposures with a maximum after 60 min of light followed by photoadaptation with longer light exposures. In addition to brlA, genes flbA-C and fluG are also light regulated, and flbA-C are required for the correct lightdependent regulation of the upstream regulator fluG. We have found that light induction of brlA required the photoreceptor complex composed of a phytochrome FphA, and the white-collar homologs LreA and LreB, and the fluffy genes flbA-C. We propose that the activation of regulatory genes by light is the key event in the activation of asexual development by light in A. nidulans.

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Genetic requirements for initiating asexual development in Aspergillus nidulans

Cited by 164 publications

References 24 publications

KapI, a non-essential member of the Pse1p/Imp5 karyopherin family, controls colonial and asexual development in Aspergillus nidulans

KapI, a non-essential member of the Pse1p/Imp5 karyopherin family, controls colonial and asexual development in Aspergillus nidulans

Relationship between Secondary Metabolism and Fungal Development

Regulation of Conidiation by Light in Aspergillus nidulans

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