Genetic relationships of Pacific abalone (Haliotidae) species determined using universal rice primer-polymerase chain reaction fingerprinting

An, Hye Suck; Park, J.Y.; Myeong, Jeong‐In; An, Cheul Min

doi:10.4238/2013.december.4.18

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…An et al [45] used 7 RAPD markers to distinguish 6 different species of Pacific abalone at the molecular level and effectively classify them into species-specific DNA fingerprint groups. The taxonomic classification of H .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and stock identification of small abalone (Haliotis diversicolor) in Taiwan and Japan

Hsu

Gwo

2017

PLoS ONE

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Small abalone (Haliotis diversicolor) is a commercially valuable species for both fisheries and aquaculture. The production of annual farmed small abalone in Taiwan, once the highest in the world, has dramatically decreased in the past 15 years, and currently, the industry is close to collapse. Understanding the genetic diversity of small abalone and developing stock identification methods will be useful for genetic breeding, restoring collapsed stocks, managing stocks, and preventing illegal trade. We investigated 307 cultured and wild individuals from Taiwan, Japan, and Bali Island (Indonesia) by using the mitochondrial cytochrome c oxidase subunit I (COI) gene. Network analysis of mtDNA COI gene sequences revealed that the individuals collected from Taiwan, Japan, and Indonesia could be identified, and showed significant genetic divergence. In addition, the Indonesian population (Haliotis diversicolor squamata) was significantly different from the other populations and might need to be considered a separate species. We discovered a single nucleotide polymorphism marker in the mtDNA COI gene that can be used to distinguish the Taiwan population from the Japan population. We also developed a polymerase chain reaction-restriction fragment length polymorphism method for rapid detection. Furthermore, we could identify the cultured stocks, wild population, and hybrid stocks by using 6 microsatellites and amplified fragment length polymorphism. This study contributes useful tools for stock identification and the production of high-disease resistant small abalone strains (Japan × Taiwan or Taiwan × Japan). Efforts should be made to avoid unintentional random genetic mixing of the Taiwan population with the Japan population and subsequent breakdown of population differentiation, which impair local adaptation of the Taiwan wild population. Molecular markers revealed a split between the Taiwan and Japan populations, and the existence of a possible barrier to the free dispersal of small abalone is discussed.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity and stock identification of small abalone (Haliotis diversicolor) in Taiwan and Japan

Hsu

Gwo

2017

PLoS ONE

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“…Despite that, sequences homologous to URP over a continuous range of 15 bases were widely observed on diverse genomes including Arabidopsis , human and bacteria [ 28 ]. Thus, URP primers were used for fingerprinting other organisms [ 28 ], and were proved a useful tool for genetic characterization and grouping of most eukaryotic or prokaryotic genomes, especially at inter- and intraspecific levels [ 31 , 32 ]. The URP are listed in Table 5 .…”

Section: Methodsmentioning

confidence: 99%

In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons

Tsialtas

Patelou

Kaloumenos

et al. 2014

J of Biol Res-Thessaloniki

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BackgroundThe present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.ResultsPrincipal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.ConclusionsThe findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

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Molecular and Physiological Aspects of Breeding Program for Development of Hybrids between Abalones Distributed in the Coast of Korea

Lee¹,

Seo²,

Kim³

et al. 2016

Journal of Life Science

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Abalones are gastropod mollusks belonging to the genus Haliotis. Fishery products are continuously declining worldwide while abalone production from aquatic farms has considerably increased. Although abalones are regarded as very important marine resources and considered to have big potential in sea-food farming industry around world, the slow growth rate of Pacific abalones is considered to be one of the most serious problems. For the genetic improvements in cultured abalone, advances in various breeding techniques for abalone have been reached through the introduction of selection, crossbreeding, hybridization, and polyploidy in several commercially important abalone species. Six species of abalone have been reported to be distributed along the coasts of Korea: Haliotis discus hannai, Haliotis discus discus, Haliotis madaka, Haliotis gigantea, Haliotis diversicolor diversicolor, and Haliotis diversicolor supertexta. The hybridization between these abalones may be one of the advanced technologies, and the preliminary experiments of interspecific hybrids between abalone species distributed in northern pacific areas including Korea, China, and Japan have been conducted. In this study, we reviewed the phylogenetic relationship of northern pacific abalone species which have the potential traits for aquaculture in Korea and their identifications. We also examined the development of molecular markers and some other aspects of the genetic approaches for successful development of hybrids.

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Genetic relationships of Pacific abalone (Haliotidae) species determined using universal rice primer-polymerase chain reaction fingerprinting

Cited by 3 publications

References 23 publications

Genetic diversity and stock identification of small abalone (Haliotis diversicolor) in Taiwan and Japan

Genetic diversity and stock identification of small abalone (Haliotis diversicolor) in Taiwan and Japan

In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons

Molecular and Physiological Aspects of Breeding Program for Development of Hybrids between Abalones Distributed in the Coast of Korea

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