Genetic Relationships in Advanced Generation Hybrids Derived from Crosses between Texas (&amp;lt;i&amp;gt;Poa arachnifera&amp;lt;/i&amp;gt;) and Kentucky (&amp;lt;i&amp;gt;Poa pratensis&amp;lt;/i&amp;gt;) Bluegrass Using ISSR Markers

Goldman, J. J.

doi:10.4236/ajps.2013.412303

Cited by 5 publications

(3 citation statements)

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“…In grasses, RAPD and ISSR markers are most commonly used to evaluate genetic variability within and between populations, and for molecular characterization and identification of different species and their hybrids, cultivars and genotypes ( Posselt et al, 2006 ; Pivorienė et al, 2008 ; Al-Humaid, Ibrahim & Motawei, 2011 ; Motawei & Al-Ghumaiz, 2012 ; Madesis et al, 2014 ; Yuan et al, 2014 ). In P. pratensis , genetic analyses employing RAPD and ISSR focus mainly on the identification of sexual and apomictic genotypes ( Huff & Bara, 1993 ; Mazzucato et al, 1995 ; Barcaccia et al, 1997 ; Barcaccia, Veronesi & Falcinelli, 1998 ; Stephens et al, 2006 ); the assessment of genetic variability and identification of genotypes and cultivars ( Lickfeldt, Voigt & Hamblin, 2002 ; Ning et al, 2005 ; Liang et al, 2009 ; Fard et al, 2012 ; Wang et al, 2012 ; Yuan et al, 2015 ); determining the genetic relationships between different genotypes and species belonging to the genus Poa and their hybrids ( Johnson et al, 2002 ; Curley & Jung, 2004 ; Patterson, Larson & Johnson, 2005 ; Goldman, 2008 ; Goldman, 2013 ); and identification of pathogens responsible for fungal diseases in grasses ( Hsiang et al, 2000 ). Both marker systems can generate high resolution band patterns and high levels of polymorphism as high as 90%.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Szenejko

Śmietana

Stępień

2016

PeerJ

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BackgroundPoa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation.MethodsThe molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA).ResultsThe low value of Dice’s coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin.DiscussionThe populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Szenejko

Śmietana

Stępień

2016

PeerJ

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“…Identification of 'Mianli' progeny with ISSR molecular markers. With ISSR markers, nearly identical amplified bands were obtained for D4-10 × Poland single plant DNA and DNA derived from turfgrass started from seedlings on all gels, which again showed that D4-10 × Poland is highly apomictic (Goldman, 2013). We conducted DNA analyses on 'Mianli' progeny after emasculation with bagging using ISSR marker technology.…”

Section: Discussionmentioning

confidence: 83%

A Preliminary Study of Apomictic Characteristics of ‘Mianli’ (Pyrus sinkiangensis Yü)

Zhou

et al. 2022

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This study investigated the ploidy of ‘Mianli’ with flow cytometry and the traditional chromosome squash technique. Its pollination biology and the occurrence and formation of embryo sacs before and after flowering were observed in paraffin sections to characterize its embryo sacs. The intersimple sequence repeat (ISSR) marker technique was used to test the uniformity of progeny of ‘Mianli’ treatments. The chromosome number of ‘Mianli’ is 2n = 2x = 34. The ploidy results were consistent with those identified by flow cytometry. ‘Mianli’ is male-sterile, and the anatropous ovule has double integuments. ‘Mianli’ can bear fruit normally and produce fertile seeds under the treatments of emasculation with bagging or no emasculation with bagging, but the seed yield is very low and significantly lower than that under artificial pollination or natural pollination. The developmental process of embryo sacs under natural pollination showed that most megasporocytes develop into mature sexual embryo sacs through meiosis and a few megasporocytes degenerate. Some sexual embryo sacs continue to develop into embryos after fertilization, and some sexual embryo sacs are aborted. In addition, new aposporous initial cells are generated irregularly at each stage from the emergence of megasporocyte to the end of sexual reproduction or abortion. The observation of the development of embryo sacs under emasculation with bagging showed that after pollination is blocked, mature sexual embryo sacs degenerate, and aposporous mononucleate embryo sacs appear around the degenerated sexual embryo sacs or in the peripheral tissues. Then, the process of proembryonic masses developing into spherical embryo was observed. A genetic uniformity analysis of progeny of ‘Mianli’ using ISSR was performed. The results showed that the progeny population under emasculation with bagging has high consistency at the molecular level, with some plants having full consistency with the female parent’s banding pattern, demonstrating consistency with the maternal genetic characteristics. The progeny under artificial pollination or natural pollination do not have the same banding pattern as the female parent. Because there is no pseudogamy, all of the progeny are true hybrids. In summary, it seems that ‘Mianli’ only has sexual reproduction in the presence of pollen, and only a few ovules are stimulated to undergo apomixis after pollination is blocked.

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“…In grasses, RAPD and ISSR markers are most commonly used to evaluate genetic variability within and between populations, and for molecular characterization and identification of different species and their hybrids, cultivars and genotypes (Posselt et al, 2006;Pivorienė et al, 2008;Al-Humaid et al, 2011;Motawei & Al-Ghumaiz, 2012;Madesis et al, 2014;Yuan et al, 2014). In P. pratensis genetic analyses employing RAPD and ISSR focus mainly on the identification of sexual and apomictic genotypes (Huff & Bara, 1993; PeerJ reviewing PDF | (2016:04:10046:1:0:NEW 12 Aug 2016) Manuscript to be reviewed et al, 1995;Barcaccia et al, 1997Barcaccia et al, , 1998Stephens et al, 2006); the assessment of genetic variability and identification of genotypes and cultivars (Lickfeldt et al, 2002;Ning et al, 2005;Liang et al, 2009;Fard et al, 2012;Wang et al, 2012;Yuan et al, 2015); determining the genetic relationships between different genotypes and species belonging to the genus Poa and their hybrids (Johnson et al, 2002;Curley & Jung, 2004;Patterson et al, 2005;Goldman, 2008;Goldman, 2013); and identification of pathogens responsible for fungal diseases in grasses (Hsiang et al, 2000). Both marker systems can generate high resolution band patterns and high levels of polymorphism as high as 90%.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers (v0.1)"

2016

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Background. Poa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation. Methods. The molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA). Results. The low value of Dice's coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin. Discussion. The populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability.

show abstract

Genetic Relationships in Advanced Generation Hybrids Derived from Crosses between Texas (<i>Poa arachnifera</i>) and Kentucky (<i>Poa pratensis</i>) Bluegrass Using ISSR Markers

Cited by 5 publications

References 6 publications

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

A Preliminary Study of Apomictic Characteristics of ‘Mianli’ (Pyrus sinkiangensis Yü)

Peer Review #1 of "Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers (v0.1)"

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