Genetic relationships in a germplasm collection of Camellia japonica and Camellia oleifera using SSR analysis

Zhao, Yaqian; Ruan, Chengjiang; Ding, Ge; Mopper, Kenneth

doi:10.4238/gmr16019526

Cited by 13 publications

(8 citation statements)

References 38 publications

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“…5 ). Similar results were obtained in other studies that distinguished C. japonica cultivars from other cultivars in the genus Camellia using SSR markers 6 , 26 . A recent study in C. nitidissima grouped 96 individuals into 4 subpopulations and found some overlap between subpopulations 27 .…”

Section: Discussionsupporting

confidence: 90%

“…Therefore, the newly developed SSR markers as the core markers had high power for Camellia genotyping. Several previous studies showed a significant positive correlation between the genetic distance and geographic distance of ornamental camellia populations 26 , 27 , 36 . In the present study, we found that accessions with the similar flower color were grouped together in several clusters/subclusters in the dendrogram (Fig.…”

Section: Discussionmentioning

confidence: 79%

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Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia

et al. 2021

Sci Rep

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Camellia is a genus of flowering plants in the family Theaceae, and several species in this genus have economic importance. Although a great deal of molecular makers has been developed for molecular assisted breeding in genus Camellia in the past decade, the number of simple sequence repeats (SSRs) publicly available for plants in this genus is insufficient. In this study, a total of 28,854 potential SSRs were identified with a frequency of 4.63 kb. A total of 172 primer pairs were synthesized and preliminarily screened in 10 C. japonica accessions, and of these primer pairs, 111 were found to be polymorphic. Fifty-one polymorphic SSR markers were randomly selected to perform further analysis of the genetic relationships of 89 accessions across the genus Camellia. Cluster analysis revealed major clusters corresponding to those based on taxonomic classification and geographic origin. Furthermore, all the genotypes of C. japonica separated and consistently grouped well in the genetic structure analysis. The results of the present study provide high-quality SSR resources for molecular genetic breeding studies in camellia plants.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 79%

Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia

et al. 2021

Sci Rep

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“…Molecular methods, e.g. DNA and RNA sequences [10, 17–24], internal transcribed spacer [10, 18], simple sequence repeats (SSR) [25, 26], ribosomal DNA [27] and several DNA loci [28], have been involved to better understand the evolution of the Camellia . A number of studies focus on the taxonomy, species identification and phylogenetics of the Camellia , but still have not get a satisfied resolution.…”

Section: Introductionmentioning

confidence: 99%

Complete chloroplast genome of Camellia japonica genome structures, comparative and phylogenetic analysis

Guo

Liu

2019

PLoS ONE

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Camellia is an economically, ecologically and phylogenetically valuable genus in the family Theaceae. The frequent interspecific hybridization and polyploidization makes this genus phylogenetically and taxonomically under controversial and require detailed investigation. Chloroplast (cp) genome sequences have been used for cpDNA marker development and genetic diversity evaluation. Our research newly sequenced the chloroplast genome of Camellia japonica using Illumina HiSeq X Ten platform, and retrieved five other chloroplast genomes of Camellia previously published for comparative analyses, thereby shedding lights on a deeper understanding of the applicability of chloroplast information. The chloroplast genome sizes ranged in length from 156,607 to 157,166 bp, and their gene structure resembled those of other higher plants. There were four categories of SSRs detected in six Camellia cpDNA sequences, with the lengths ranging from 10 to 17bp. The Camellia species exhibited different evolutionary routes that lhbA and orf188, followed by orf42 and psbZ, were readily lost during evolution. Obvious codon preferences were also shown in almost all protein-coding cpDNA and amino acid sequences. Selection pressure analysis revealed the influence of different environmental pressures on different Camellia chloroplast genomes during long-term evolution. All Camellia species, except C . crapnelliana , presented the identical rate of amplification in the IR region. The datasets obtained from the chloroplast genomes are highly supportive in inferring the phylogenetic relationships of the Camellia taxa, indicating that chloroplast genome can be used for classifying interspecific relationships in this genus.

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“…The identification of Camellia species and cultivars is mainly based on the morphological features of flowers and leaves and, to a lesser extent, on the type of plant growth. However, all these features can be affected by ontogenetic and environmental factors [20] [21]. Some morphological characteristics, especially those of flowers, are highly variable among specimens belonging to the same cultivar and even among flowers of the same plant, which makes the visual and subjective evaluation of the researcher very difficult [9].…”

Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Study of Hundred-Year-Old Camellias Growing at Pazo de Lourizán, Pontevedra (NW-Spain)

Salinero¹,

Vela²,

Barros³

et al. 2020

IJFSA

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Genetic relationships in a germplasm collection of Camellia japonica and Camellia oleifera using SSR analysis

Cited by 13 publications

References 38 publications

Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia

Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia

Complete chloroplast genome of Camellia japonica genome structures, comparative and phylogenetic analysis

Morphological and Molecular Study of Hundred-Year-Old Camellias Growing at Pazo de Lourizán, Pontevedra (NW-Spain)

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