Genetic relationships between populations of Aegilops tauschii Coss. (Poaceae) using SCoT molecular markers

Abstract

The genus Aegilops has an important potential utilization in wheat improvement because of its resistance to different biotic and abiotic stresses and close relation with the cultivated wheat. Aegilops tauschii grows in Iran, westward to Turkey and eastward to Afghanistan and China with a distribution center in the south of Caspian Sea. In spite of its very good biochemical characterization, the knowledge about the DNA variability is very limited and no DNA markers were used to analyses the genomic variability of the populations, up to date. In the present study, genetic diversity of 117 Aegilops tauschii, individuals nine populations were studied using 10 Start Codon Targeted (SCoT) markers. High polymorphic bands (96.33%), polymorphic information content (0.48) and allele number (1.024) showed SCoT as a reliable marker system for genetic analysis in Aegilops tauschii. At the species, the percentage of polymorphic loci [P] was 66.30%, Nei’s gene diversity [H] was 0.35, Shannon index [I] was 0.33 and unbiased gene diversity [UHe] was 0.37. Genetic variation within populations (59%) was higher than among populations (41%) based on analysis of molecular variance (AMOVA). We used SCoT molecular marker for our genetic investigation with the following aims: 1— Investigate genetic diversity both among and with date Aegilops tauschii, 2—Identify genetic groups within these nine populations Aegilops tauschii, and 3—produce data on the genetic structure of date Aegilops tauschii populations. The results obtained revealed a high within-population genetic variability.

Assessment of Genetic Relation for Different Ploidy Levels of Aegilops and Triticum Possessing Different Genome-Bearing Species using Start Codon Target Marker

“…Different marker techniques have been applied, including random DNA markers to functional and gene-targeted markers, to study the genetic relationship between Triticum and Aegilops (Asaf et al 2022;Poczai et al 2013;Sansaloni et al 2020). Start codon-targeted (SCoT) polymorphism as a novel, simple, and reliable gene-targeted marker system are among the markers used (Nouri et al 2021;Xia et al 2022). The system is based on the short-conserved region and can use a single primer designed to anneal flanking regions of the translation initiation codon (ATG) on both DNA strands (Collard and Mackill 2009).…”