Genetic Relationships and Cross Pathogenicities of<i>Verticillium dahliae</i>Isolates from Cauliflower and Other Crops

Subbarao, Krishna V.; Chassot, A.; Gordon, Thomas R.; Hubbard, J. C.; Bonello, Pierluigi; Mulliν, Rick; Okamoto, D.; Davis, R. M.; Koike, S. T.

doi:10.1094/phyto-85-1105

Cited by 96 publications

(80 citation statements)

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“…Indeed, some studies point to a worldwide, prevalent distribution of VCG1, VCG2, and VCG4, suggesting a limited VCG diversity. Other investigations suggested that some VCGs may prevail on a crop in a given geographic area (Joaquim and Rowe 1991;Strausbaugh 1993;Katan 2000b;Subbarao et al 1995;Dobinson et al 1998;Elena 2000;Korolev et al 2000;Bhat et al 2003;Hiemstra and RatajGuranowska 2003). On the other hand, considering VCGs as fully genetically isolated groups may be a strict statement.…”

Section: Pathogenicity Groupsmentioning

confidence: 99%

“…isolates has been carried out by restriction fragment length polymorphism (RFLP) (Carder and Barbara 1991;Typas et al 1992;Carder et al 1994;Okoli et al 1994;Dobinson et al 2000), random amplified polymorphic DNA (RAPD) (Messner et al 1996;Koike et al 1996;Zeise and von Tiedemann 2002;Bhat and Subbarao 1999), and amplified fragment length polymorphism (AFLP) (Collins et al 2003;Fahleson et al 2003;Radišek et al 2003;ColladoRomero et al 2006). Analysis and comparison of DNA sequences such as the intergenic spacer (IGS) and internal transcribed spacer (ITS) regions of genes encoding ribosomal RNA, as well as repeated DNA sequences, have also been used to study variation within V. dahliae (Morton et al 1995a, b;Subbarao et al 1995;Dobinson et al 1998;Pramateftaki et al 2000).…”

Section: Pathogenicity Groupsmentioning

confidence: 99%

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Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

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Olive (Olea europaea L.) is one of the first domesticated and cultivated tree species and has historical, social and economical relevance. However, its future as a strategic commodity in Mediterranean agriculture is threatened by diverse biotic (traditional and new/emerging pests and diseases) and abiotic (erosion, climate change) menaces. These problems could also be of relevance for new geographical areas where olive cultivation is not traditional but is increasingly spreading (i.e., South America, Australia, etc). One of the major constraints for olive cultivation is Verticillium wilt, a vascular disease caused by the soil-borne fungus Verticillium dahliae Kleb. In this review we describe how Verticillium wilt of olive (VWO) has become a major problem for olive cultivation during the last two decades. Similar to other vascular diseases, VWO is difficult to manage and single control measure are mostly ineffective. Therefore, an integrated disease management strategy that fits modern sustainable agriculture criteria must be implemented. Multidisciplinary research efforts and advances to understand this pathosystem and to develop appropriate control measures are summarized. The main conclusion is that a holistic approach is the best strategy to effectively control VWO, integrating biological, chemical, physical, and cultural approaches.

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Section: Pathogenicity Groupsmentioning

confidence: 99%

Section: Pathogenicity Groupsmentioning

confidence: 99%

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

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“…To the author's knowledge, no other publication has to date focussed on determining genetic variability between V. dahliae isolates from olive, cocoa and sunflower. A previous study by Subbarao et al (1995) case, more than one IGS haplotype was detected in strains pathogenic to a particular host or pertaining to a VCG group. The presence of two IGS haplotypes in strains from cauliflower contradicted the assumption of a recent introduction of the pathogen to California.…”

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confidence: 69%

Variability in ribosomal DNA genic and spacer regions in Verticillium dahliae isolates from different hosts

2004

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Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate from sunflower. Ribosomal DNA ITS restriction patterns were identical for all isolates of V. dahliae, irrespective of host of origin. These preliminary results may have relevance for Verticillium wilt control practices, possibly reflecting a different evolutionary origin, or reproductive isolation of the pathogen in olive, distinct from populations of other hosts.

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“…longisporum (47,48). Since then, many similar isolates from crucifer hosts have been reported (20,22,40,49,50). Because the ma-jority of these long-spored isolates from cruciferous hosts possess nearly double the nuclear DNA content of short-spored isolates (average 1.8-fold greater than that of short-spored isolates) (10,20,22,49), they have also been referred to as diploid (20), near-diploid (22), allodiploid, or amphidiploid (10).…”

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confidence: 99%

“…However, the work of Collins et al has discouraged the use of the new nomenclature since the full complexity and the derivation of those long-spored isolates are still unknown (10). Some reports indicated that short-spored isolates could be grouped with long-spored isolates at the molecular level (49) and that long-spored isolates were not truly host-specific (8,21,50,56) as previously described (22). Because of the inconsistencies in the results of the above studies with those of Karapapa's (22), the appropriateness of the name V. longisporum has been questioned even if it is regarded as a distinct new species (13,56).…”

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confidence: 99%

Phylogenetic Analyses of Phytopathogenic Isolates of Verticillium spp.

Qin¹,

Vallad²,

Wu³

et al. 2006

Phytopathology®

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To better understand the genetic relationships between Verticillium dahliae isolates from lettuce and other phytopathogenic Verticillium spp. isolates from various hosts and geographic locations, the complete intergenic spacer (IGS) region of the nuclear ribosomal RNA gene (rDNA) and the β-tubulin gene were amplified and sequenced. The sequences of the complete IGS region and the β-tubulin gene were used alone and in combination to infer genetic relationships among different isolates of Verticillium with the maximum-likelihood distance method. Phylogenetic analyses set sequences into four distinct groups comprising isolates of V. albo-atrum, V. tricorpus, and V. dahliae from cruciferous and noncruciferous hosts. Within the four Verticillium groups, isolates of V. dahliae from cruciferous hosts displayed the closest affinity to V. dahliae from noncruciferous hosts. Isolates of V. dahliae from noncruciferous hosts could be further divided into four subgroups based on sequence similarities within the IGS region. Cross-pathogenicity tests demonstrated that most Verticillium isolates were as virulent on other hosts as on their hosts of origin. A phenogram based on the cross pathogenicity of individual isolates resembled those derived from the IGS and β-tubulin sequence comparisons. On the basis of the data presented, the potential origin of some isolates of V. dahliae pathogenic on lettuce is proposed.

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Genetic Relationships and Cross Pathogenicities ofVerticillium dahliaeIsolates from Cauliflower and Other Crops

Cited by 96 publications

References 0 publications

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

Variability in ribosomal DNA genic and spacer regions in Verticillium dahliae isolates from different hosts

Phylogenetic Analyses of Phytopathogenic Isolates of Verticillium spp.

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