Genetic relationships among populations of Aedes aegypti from Uruguay and northeastern Argentina inferred from ISSR-PCR data

Abstract

Aedes aegypti (L.) (Diptera: Culicidae), the main vector of yellow fever and dengue viruses, was eradicated from Argentina between 1955 and 1963, but reinvaded the country in 1986. In Uruguay, the species was reintroduced in 1997. In this study we used highly polymorphic inter-simple sequence repeats (ISSR) markers to analyse the genetic structure of Ae. aegypti populations from Uruguay and northeastern Argentina to identify possible colonization patterns of the vector. Overall genetic differentiation among populations was high (F(ST) = 0.106) and showed no correlation with geographic distance, which is consistent with the short time since the reintroduction of the species in the area. Differentiation between pairs of Argentine populations (F(ST) 0.072 to 0.221) was on average higher than between Uruguayan populations (F(ST)-0.044 to 0.116). Bayesian estimation of population structure defined four genetic clusters and most populations were admixtures of two of them: Mercedes and Treinta y Tres (Uruguay) were mixtures of clusters 1 and 3; Salto (Uruguay) and Paraná (Argentina) of clusters 1 and 4; Fray Bentos (Uruguay) of clusters 2 and 3, and Gualeguaychú (Argentina) of clusters 2 and 3. Posadas and Buenos Aires in Argentina were fairly genetically homogeneous. Our results suggest that Ae. aegypti recolonized Uruguay from bordering cities in Argentina via bridges over the Uruguay River and also from Brazil.

Application of Inter-Simple Sequence Repeat Markers in the Analysis of Populations of the Chagas Disease Vector Triatoma infestans (Hemiptera, Reduviidae)

“…In Aedes aegypti and Culex quinquefasciatus were detected higher levels of genetic diversity using ISSR markers, with an average value of uH of 0.31 and an average value of the Shannon index of 0.36, respectively. 16,17 However, although the levels of genetic diversity detected in this study were moderate, it is important to point out that the analysis of a greater number of primers will allow to explore a greater proportion of the genome, increasing the potential of ISSR markers for the analysis of T. infestans populations.…”

Aedes aegyptifrom temperate regions of South America are highly competent to transmit dengue virus

“…Interestingly, Ae. aegypti populations from Buenos Aires and Corrientes in Argentina as well as Salto in Uruguay are not very genetically differentiated, suggesting an important gene flow between populations from the Southern Cone [11,18,30]. Collectively, data on genetic population studies implied that Ae.…”

Genetic structure of Spanish populations of Ceratitis capitata revealed by RAPD and ISSR markers: implications for resistance management

“…Raina et al, 2001;Martins-Lopes et al, 2007) and to a lesser extent in insects (e.g. Ochando et al, 2010;Soliani et al, 2010).…”

“…Raina et al, 2001;Martins-Lopes et al, 2007) and to a lesser extent in insects (e.g. Ochando et al, 2010;Soliani et al, 2010).In this paper we examine for the first time the genetic variation of C. capitata populations, by two molecular approaches, RAPD and ISSR analysis. The aim of the present work was, first, to compare both molecular methodologies to detect geographic patterns of genetic variation in this pest, and second, to get information about the genetic structure of Spanish populations that would assist in designing strategies for medfly control and provide some insights in decision making for resistance management.…”