1997
DOI: 10.1099/00221287-143-8-2841
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Genetic relationships among Pasteurella trehalosi isolates based on multilocus enzyme electrophoresis

Abstract: Genetic diversity among 60 British Pasteurella trehalosi isolates representing the four recognized capsular serotypes, T3, T4, T I 0 and T15, and recovered predominantly from sheep suffering from systemic pasteurellosis, was estimated by analysing electrophoretically demonstrable allelic variation a t structural genes encoding 19 enzymes. Thirteen of the loci were polymorphic and 20 distinctive multilocus genotypes (electrophoretic types, ETs) were identified. The population structure of P. trehalosi is clonal… Show more

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Cited by 17 publications
(13 citation statements)
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“…The complete nucleotide sequence of the lktA gene was determined for 31 isolates of M. haemolytica representing 12 capsular serotypes and the 22 electrophoretic types (ETs) previously defined by multilocus enzyme electrophoresis (MLEE) (13). The lktA gene was also sequenced for six isolates representing different ETs of M. glucosida (13) and for four isolates that each represent one of the capsular serotypes (T3, T4, T10, and T15) of P. trehalosi (14). Part of the lktA gene was also sequenced for an additional 13 serotype A2 isolates of M. haemolytica recovered from cattle and sheep in order to identify the allele type.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The complete nucleotide sequence of the lktA gene was determined for 31 isolates of M. haemolytica representing 12 capsular serotypes and the 22 electrophoretic types (ETs) previously defined by multilocus enzyme electrophoresis (MLEE) (13). The lktA gene was also sequenced for six isolates representing different ETs of M. glucosida (13) and for four isolates that each represent one of the capsular serotypes (T3, T4, T10, and T15) of P. trehalosi (14). Part of the lktA gene was also sequenced for an additional 13 serotype A2 isolates of M. haemolytica recovered from cattle and sheep in order to identify the allele type.…”
Section: Resultsmentioning
confidence: 99%
“…Partial lktA sequences were obtained for a further 13 serotype A2 M. haemolytica isolates of bovine and ovine origin. The 54 isolates have been well characterized in previous studies (10)(11)(12)(13)(14) and were chosen to represent selected evolutionary lineages, serotypes, and hosts of origin. Properties of these isolates are presented in Table 1.…”
Section: Methodsmentioning
confidence: 99%
“…The UT isolates may represent either capsule-deficient strains or distinct species [6,19]. Furthermore, extensive epidemiological studies of M. haemolytica strains using molecular typing techniques have shown some significant level of heterogeneity even within strains of the same serovar isolated from various hosts and also from different geographical areas [7,8,16].…”
Section: Introductionmentioning
confidence: 99%
“…The ompA genes from 31 M. haemolytica isolates representing 12 capsular serotypes and 22 electrophoretic types (ETs) previously defined by multilocus enzyme electrophoresis (11) were sequenced. The ompA genes from six isolates representing different ETs of M. glucosida (11) and from four isolates representing each of the capsular serotypes T3, T4, T10, and T15 of P. trehalosi (12) were also sequenced. PCR errors were shown to be insignificant by duplicate amplification and sequencing of ompA for strains PH2, PH376, and PH344.…”
Section: Resultsmentioning
confidence: 99%
“…The ompA genes from 31 M. haemolytica, 6 M. glucosida, and 4 P. trehalosi isolates were sequenced. The 41 strains were well characterized in previous studies (11)(12)(13)(14)(15)(16)(17) and were selected to represent specific evolutionary lineages, capsular serotypes, and hosts of origin. Some properties of these isolates are presented in Table 1.…”
Section: Methodsmentioning
confidence: 99%