Genetic Relationships Among 22 Taxa of Bamboo Revealed by ISSR and EST-Based Random Primers

Abstract

Genetic relationships among 22 taxa of bamboo were evaluated using 12 inter simple sequence repeats (ISSR) and four expressed sequence tag (EST)-based random primers, resulting in amplification of 220 loci. The grouping of species based on Jaccard's similarity matrix using UPGMA and principal coordinate analysis agreed with earlier published reports on molecular phylogenetic studies in bamboos with few deviations. In the dendrogram, however, species of one genus were placed in different clusters along with members of other genera. This calls for correct taxonomic delineation at the genus and species level using both vegetative and reproductive characters and correlation of molecular data with morphologically definable taxonomic groupings at the proper taxonomic level.

Distribution, Identification and Genetic Diversity among Bamboo Species: A Phenomic Approach

“…22 But, Incidence of flowering of woody bamboo is uncertain. 23,24 As reported earlier, the reproductive cycle of bamboo is too long from 3years to 120years. 25 That is why the identification depending on reproductive structure is difficult.…”

Bamboo: an overview on its genetic diversity and characterization

“…Bamboo has age-old connection with the material needs of rural people (Mukherjee et al 2010 ). Porter-field ( 1933 ) suggested that “bamboo is one of those providential developments in nature which, like the horse, the cow, wheat and cotton, have been indirectly responsible for man’s own evolution”.…”

“…In case of gregarious flowering, all the members of a common cohort (plants from seeds of common origin) go into reproductive phase simultaneously and subsequently die (Bhattacharya et al 2009 ). Incidence of flowering of woody bamboo is uncertain (Ramanayake et al 2007 ; Mukherjee et al 2010 ). As reported earlier, the reproductive cycle of bamboo is too long, from 3 to 120 years (Janzen 1976 ), hence making the identification depending on reproductive structure difficult (Bhattacharya et al 2006 , 2009 ).…”

“…ISSR technique has been employed for phylogenetic relationship study in many crops such as rice (Joshi et al 2000 ; Qian et al 2001 ). ISSR has become a popular technique for genetic relationship study by many scientists working on bamboo (Lin et al 2010 ; Mukherjee et al 2010 ).…”

Phylogenetics, systematics and evolution of the temperate woody bamboos with an emphasis on the Kuruna clade

“…This analysis is the first study investigating the genetic diversity and structure of K. debilis from Sri Lanka, with samples from almost all known populations evaluated using microsatellite markers. Although several studies have previously quantified population level genetic variation in bamboos, none of them have related this variation to the conservation of bamboo populations or species (Nayak et al, 2003;Ramanayake et al, 2007;Lalhruaitluanga & Prasad, 2009;Mukherjee et al, 2010;.…”