Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method

Abstract

The PCR based RAPD analysis was used to study genetic relationship among roselle and kenaf accessions. Extraction of genomic DNA samples of nine roselle (Hibiscus sabdariffa L.) and seven kenaf (Hibiscus cannabinus L.) accessions were successfully performed by Cetyl Trimethyl Ammonium Bromide (CTAB) method. RAPD analysis using two chloroplast primers and one mitochondrial primer produced about 62 bands of different sizes. Most of the fragments were polymorphic where 2-3 fragments were monomorphic in each primer. Sixteen accessions were classified into two main groups A and B through dendogram generated using RAPD polymorphism. Five kenaf accessions were included in group A, whereas nine roselle accessions and two kenaf accessions were included in group B. A group was formed with a range of similarity coefficients of 0.5333 to 0.8780 having all roselle accessions. Bengkalis was different from Hibiscus sp but it was close to roselle accessions. Noonsoon was very close to roselle accessions having a range of jaccard similarity coefficients of 0.5000 to 0.6326. It was evident from jaccad similarity coefficient of 0.8780 that the roselle varieties UKMR 1 and UKMR 2 were genetically closer to each other. On the other hand, V36 and Thainung showed the highest similarity coefficient (0.7878) among kenaf accessions in group A. This study has successfully differentiated between two species by using chloroplast and mitochondrial markers which was due to high level of polymorphism. However, the chloroplast primers were more effective than M13 to study the genetic variation between roselle and kenaf.

Genetic diversity among four genotypes from Hibiscus rosasinensis in relation to drought stress

“…These results agreed with the data presented by both of Ahmed (2013) and also with Seeruttum and Sanmukiya (2013). The present study is in perfect agreement with the work of Omalsaad et al (2014), Daudu et al (2015) and Daudu et al (2016) on H. sabdariffa. It is also evident from the present results that RAPD molecular marker was able to detect small genetic differences among the different genotypes of Hibiscus rosasinensis.…”

Chemistry, phytotechnology, pharmacology and nutraceutical functions of kenaf ( Hibiscus cannabinus L.) and roselle ( Hibiscus sabdariffa L.) seed oil: An overview

“…Lectin Yang et al, 1982 ern approaches such as genetic variability analysis, correlation and path analysis, heterosis studies, combining ability and gene action, molecular breeding, use of molecular markers, development of transgenic crop, marker assisted selection, mutation breeding, artificial pollination, genetic male sterility etc. have frequently been used to assist the parental selection of breeding for producing improved quality parameters in seed oil derived from roselle and kenaf (Ryu et al, 2013;Mostofa et al, 2013;Omalsaad et al, 2014). Recent studies also demonstrated the use of roselle and kenaf seed oil as biofuel which also involves phytotechnology applications (Rathana et al, 2010;Saba et al, 2015).…”

Evaluation of Roselle Based on the Internal Transcribed Spacer Sequence of rRNA gene

“…altissima (JQ609255.1). This suggests that H. sabdraiffa and H. cannabinus cannot be distinguished using SNPs, although they were separated using RAPD markers (Omalsaad et al, 2014). Sequences of ITS 1, 2 regions of one sample each of four types were registered at NCBI GenBank: U05 (A type, KP262031), U06 (B type, KP262033), M16 (C type, KP262032), and S02 (D type, KP262034).…”

“…So several methods were performed such as randomly amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and inverse sequence-tagged repeat (ISTR) to characterize Roselle (Torres-Moran et al, 2011;Khafaga, 2013;Omalsaad et al, 2014). Hibiscus sabdariffa with 5-lobed and a mostly red calyx whereas H. cannabinus accessions with 3 -lobed leaves and mostly a yellow calyx were separated using RAPD markers, but separation of some accessions in the same species were not successful (Omalsaad et al, 2014). H. sabdariffa cultivars collected from different locations and showed three different phenotypes were divided only two group using RAPD and ISSR marker (Khafaga, 2013).…”