Genetic relatedness of Mycobacterium avium subsp. hominissuis isolates from bathrooms of healthy volunteers, rivers, and soils in Japan with human clinical isolates from different geographical areas

Arikawa, Kentaro; Ichijo, Tomoaki; Nakajima, Satomi; Nishiuchi, Yukiko; Yano, Hirokazu; Tamaru, Aki; Yoshida, Shiomi; Maruyama, Fumito; Ota, Akinobu; Nasu, Masao; Старкова, Д. А.; Mokrousov, Igor; Narvskaya, Olga; Iwamoto, Tomotada

doi:10.1016/j.meegid.2019.103923

Cited by 16 publications

(28 citation statements)

References 26 publications

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“…Uchiya et al [37] reported a difference in chromosome structure between TH135 (EA2) and Mah104 (SC2)5. Arikawa et al [10] reported the presence of MAH in the bathrooms of healthy volunteers…”

Section: Discussionmentioning

confidence: 99%

“…hominissuis (MAH) is currently the most frequently encountered subspecies [1, 2]. MAC is thought to reside in natural environments and human-built architecture including potable water systems and bathroom biofilm [3–10]. MAC is generally difficult to eradicate by antibiotic treatment, and its infection mechanisms, lineage-specific phenotypic characteristics, and pattern of short- and long-term genetic changes are very poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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The recombination-cold region as an epidemiological marker of recombinogenic opportunistic pathogen Mycobacterium avium

et al. 2019

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Background The rapid identification of lineage remains a challenge in the genotyping of clinical isolates of recombinogenic pathogens. The chromosome of Mycobacterium avium subsp. hominissuis (MAH), an agent of Mycobacterium avium complex (MAC) lung disease, is often mosaic and is composed of chromosomal segments originating from different lineages. This makes it difficult to infer the MAH lineage in a simple experimental set-up. To overcome this difficulty, we sought to identify chromosomal marker genes containing lineage-specific alleles by genome data mining. Results We conducted genetic population structure analysis, phylogenetic analysis, and a survey of historical recombination using data from 125 global MAH isolates. Six MAH lineages (EA1, EA2, SC1, SC2, SC3, and SC4) were identified in the current dataset. One P-450 gene (locus_tag MAH_0788/MAV_0940) in the recombination-cold region was found to have multiple alleles that could discriminate five lineages. By combining the information about allele type from one additional gene, the six MAH lineages as well as other M. avium subspecies were distinguishable. A recombination-cold region of 116 kb contains an insertion hotspot and is flanked by a mammalian cell-entry protein operon where allelic variants have previously been reported to occur. Hence, we speculate that the acquisition of lineage- or strain-specific insertions has introduced homology breaks in the chromosome, thereby reducing the chance of interlineage recombination. Conclusions The allele types of the newly identified marker genes can be used to predict major lineages of M. avium. The single nucleotide polymorphism typing approach targeting multiallelic loci in recombination-cold regions will facilitate the epidemiological study of MAC, and may also be useful for equivalent studies of other nontuberculous mycobacteria potentially carrying mosaic genomes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The recombination-cold region as an epidemiological marker of recombinogenic opportunistic pathogen Mycobacterium avium

et al. 2019

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“…MAC have been isolated from bathrooms [11][12][13][14][15]31] and showerheads [14,15]. Furthermore, M. avium isolates from the showerhead and the infected patient using the shower were shown to be clonally related [15].…”

Section: Mac In Premise Plumbingmentioning

confidence: 99%

“…Surveys across the United States have shown that 70% of showerheads have Mycobacterium spp., of which 30% have MAC [11][12][13][14][15]. Studies focusing specifically on mycobacteria in showerheads confirmed their presence, and identified microorganisms whose presence coincided with mycobacteria [11][12][13][14]. The rapid detection of MAC is possible using DNA amplification methods, but that information cannot be employed to compare DNA fingerprints of patient and environment isolates.…”

Section: Mac Are In Most Householdsmentioning

confidence: 99%

“…MAC are staggeringly disinfectant-resistant [5], desiccation-resistant [Falkinham, in preparation], grow at low nutrient assimilable organic carbon (AOC) concentrations [6], grow at low oxygen concentrations [7], form biofilms [8] and tolerate high water heater temperatures [9]. MAC are readily aerosolized [10], and pulmonary infections have been traced to showerheads [11][12][13][14][15]. Humans are surrounded by MAC.…”

Section: Introductionmentioning

confidence: 99%

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Mycobacterium avium Complex (MAC) in Water Distribution Systems and Household Plumbing in the United States

Falkinham

2020

Water

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Members of the Mycobacterium avium complex (MAC) are waterborne, opportunistic pathogens whose characteristics make urban water distribution systems and household plumbing ideal habitats for their survival, persistence and growth. Rather than contaminants, MAC are colonists of drinking water systems. MAC are normal inhabitants of natural soils and water, and enter drinking water treatment systems through surface sources. A proportion of MAC survive transmission through the treatment plant, and regrow in the distribution system and household plumbing. Once within household plumbing, MAC adhere to surfaces and form biofilms, thus preventing their washout. The thermal tolerance of MAC leads to growth in water heating systems. Stagnation does not reduce MAC numbers, as MAC can grow at low oxygen levels. MAC present challenges to current water monitoring approaches as their numbers do not correlate with E. coli, fecal coliforms or heterotrophic plate count bacteria.

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Unique genomic sequences in a novel Mycobacterium avium subsp. hominissuis lineage enable fine scale transmission route tracing during pig movement

Komatsu¹,

Ohya

Ota

et al. 2023

One Health

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Genetic relatedness of Mycobacterium avium subsp. hominissuis isolates from bathrooms of healthy volunteers, rivers, and soils in Japan with human clinical isolates from different geographical areas

Cited by 16 publications

References 26 publications

The recombination-cold region as an epidemiological marker of recombinogenic opportunistic pathogen Mycobacterium avium

The recombination-cold region as an epidemiological marker of recombinogenic opportunistic pathogen Mycobacterium avium

Mycobacterium avium Complex (MAC) in Water Distribution Systems and Household Plumbing in the United States

Unique genomic sequences in a novel Mycobacterium avium subsp. hominissuis lineage enable fine scale transmission route tracing during pig movement

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