Genetic Relatedness of Mexican Common Bean Cultivars Revealed by Microsatellite Markers

Abstract

Mexico has various types of common bean (Phaseolus vulgaris L.) and a long history of breeding in multiple commercial classes. In this study, our goal was to evaluate a wide collection of commercial cultivars of common beans from Mexico, including Azufrado, Bayo, Flor de Mayo, Flor de Junio, Pinto, and black bean types, to discover their level of relatedness with 32 simple sequence repeat (SSR) markers. Eight panels of end-labeled fluorescent SSR loci were used in an automated detection system and 85% of loci were read. A total of 204 alíeles were detected in the study, showing the diversity among the Mexican cultivars. Through population structure and principal components analyses, Andean and Mesoamerican genotypes as well as three subgroups within the Mesoamerican genepool were distinguished. The divisions corresponded to a group of Andean cultivars, two subgroups of the Durango-Jalisco (DJ) complex, and one group equivalent to race Mesoamerica. This latter race was much less diverse than the DJ complex and had little gene flow with other groups. This study shows the value of fluorescent SSR markers for evaluating commercial cultivars of common beans. Given the importance of Mexico as a primary center of origin for common beans, as a major market for the crop, and as the second largest producer in Latin America, this study has implications for the global evaluation of common bean cultivars.

Genetic diversity and population structure of common bean (Phaseolus vulgaris L) germplasm of Ethiopia as revealed by microsatellite markers

“…The hierarchical classification scheme into subpopulations comprised of Andean and Mesoamerican genotypes obtained here was in agreement with that reported for common bean germplasm in various studies (Singh et al, 1991a;Gepts, 1998;Diaz and Blair, 2006;Blair et al, 2007Blair et al, , 2010aBlair et al, , 2011Okii et al, 2014b). Moreover, the moderate to mostly large differentiation among subpopulations (F st values) were higher than was reported in other studies (Asfaw et al, 2009;Okii et al, 2014b).…”

Diversity and Population Structure of Common Bean from Brazil

“…The reason for these differences between studies could have been due to the mix of SSR, which are mostly multiallelic and codominant, compared to sequence characterized amplified region (SCAR) markers, which are mostly biallelic and/ or dominant, used in the previous analysis. In our study, we only used the most highly polymorphic SSRs from Blair et al (2006) that had been corroborated in other diversity studies such as those of Blair et al (2009Blair et al ( , 2010Blair et al ( , 2011. A set of only SSR markers could be considered better than the markers used by Burle et al (2010), which included many low polymorphism SCAR markers.…”

“…A total of 366 genotypes were used in this study of which 362 were from Brazil and four were control genotypes (Calima, DOR364, ICA Pijao, and G19833) used in previous diversity studies for common bean (Asfaw et al, 2009;Blair et al, 2009Blair et al, , 2010Blair et al, , 2011. The Brazilian genotypes were predominantly landraces (299 genotypes); however, the study also included some commercial cultivars (10 genotypes), improved breeding lines (nine genotypes), and genotypes of unknown Brazilian origin (44 in total).…”

Phylogeographic and population genetic analyses of Cucurbita moschata reveal divergence of two mitochondrial lineages linked to an elevational gradient

“…pepo (average F IS = 0.13; Castellanos-Morales et al, 2019). However, the inbreeding coefficient in C. moschata was lower than that reported for other domesticated plants native to Mesoamerica, such as common bean Phaseolus vulgaris L. (F IS = 0.81; Blair et al, 2011) and papaya Carica papaya (F IS = 0.58; Matos et al, 2013). Domestication and crop improvement often involve increased inbreeding (Robinson, 2008;Moyers et al, 2018).…”