Genetic Relatedness Between Two Nonculturable Mycoplasmalike Organisms Revealed by Nucleic Acid Hybridization and Polymerase Chain Reaction

Deng, Sujun; Hiruki, C.

doi:10.1094/phyto-81-1475

Cited by 159 publications

(89 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Periwinkle (Catharanthus roseus) showed virescence and mildly dwarfed, compact growth after infection (Hiruki & Chen, 1984). Dodder transmission from diseased alsike clover to Catharanthus roseus by means of Cuscuta subinclusa was achieved by using a method reported previously (Deng & Hiruki, 1991), resulting in the induction of Abbreviations: AAY, American aster yellows; AshY, ash yellows; AWB, alfalfa witches'-broom; AY, aster yellows; BLL, brinjal little leaf; BLTV, beet leafhopper-transmitted virescence; CP, clover proliferation; CP R , CP reference strain; CPh, clover phyllody; EAY, eastern aster yellows; EY, elm yellows; FD, flavescence doré e of grapevine; FM, Fragaria multicipita phytoplasma; HD, hemp dogbane; HMA, heteroduplex mobility assay; HV, hydrangea virescence; ILEY, Illinois elm yellows; JWB_F, jujube witches'-broom Fukui isolate; JWB_G, jujube witches'-broom Gifu isolate; JWB_Ko, jujube witches'-broom Korea isolate; JWB_Ky, jujube witches'-broom Kyoto isolate; LWB, lilac witches'-broom; PWB, potato witches'-broom; PY, potato yellows; TBBc, tomato big bud in California; WVEY, elm yellows. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene and 16S-23S spacer region sequence of the clover proliferation phytoplasma is AY390261.…”

Section: Biological Properties Of the Cp R Phytoplasmamentioning

confidence: 99%

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

Hiruki

Wang

2004

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Clover proliferation phytoplasma (CP R ) is designated as the reference strain for the CP phylogenetic group or subclade, on the basis of molecular analyses of genomic DNA, the 16S rRNA gene and the 16S-23S spacer region. Other strains related to CP R include alfalfa witches'-broom (AWB), brinjal little leaf (BLL), beet leafhopper-transmitted virescence (BLTV), Illinois elm yellows (ILEY), potato witches'-broom (PWB), potato yellows (PY), tomato big bud in California (TBBc) and phytoplasmas from Fragaria multicipita (FM). Phylogenetic analysis of the 16S rRNA gene sequences of BLL, CP R , FM and ILEY, together with sequences from 16 other phytoplasmas that belong to the ash yellows (AshY), jujube witches'-broom (JWB) and elm yellows (EY) groups that were available in GenBank, produced a tree on which these phytoplasmas clearly clustered as a discrete group. Three subgroups have been classified on the basis of sequence homology and the collective RFLP patterns of amplified 16S rRNA genes. AWB, BLTV, PWB and TBBc are assigned to taxonomic subgroup CP-A, FM belongs to subgroup CP-B and BLL and ILEY are assigned to subgroup CP-C. Genetic heterogeneity between different isolates of AWB, CP R and PWB has been observed from heteroduplex mobility assay analysis of amplified 16S rRNA genes and the 16S-23S spacer region. Two unique signature sequences that can be utilized to distinguish the CP group from others were present. On the basis of unique properties of the DNA from clover proliferation phytoplasma, the name 'Candidatus Phytoplasma trifolii' is proposed for the CP group.

show abstract

Section: Biological Properties Of the Cp R Phytoplasmamentioning

confidence: 99%

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

Hiruki

Wang

2004

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…The development of universal PCR primer pairs designed to amplify all known phytoplasma 16S rDNA has facilitated this approach significantly (Ahrens & Seemu$ ller, 1992 ;Deng & Hiruki, 1991 ;Gundersen & Lee, 1996 ;Namba et al, 1993a). Moreover, nucleotide sequence analyses of the amplified 16S rDNA fragments have made phylogenetic analysis of phytoplasma isolates possible, and the information obtained has been used as the basis for delineating phytoplasmas into several groups (Kuske & Kirkpatrick, 1992 ;Schneider et al, 1995 ;Seemu$ ller et al, 1998 ;White et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

'Candidatus Phytoplasma castaneae', a novel phytoplasma taxon associated with chestnut witches' broom disease.

Jung¹,

Sawayanagi²,

Kakizawa³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

“…Universal phytoplasma-specific primers were used for amplification of 16S rRNA gene sequences; primers used were P1/P7 (direct PCR) (Deng and Hiruki 1991;Schneider et al 1995), followed by fU5/rU3 (nested PCR) (Seemüller et al 1994). Direct PCR amplification of rp gene sequences was primed by rpL2-F3/rp(1)-R1A (Martini et al 2007).…”

mentioning

confidence: 99%

Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico

Caicedo

Rivera-Vargas

Segarra

et al. 2015

Australasian Plant Dis. Notes

View full text Add to dashboard Cite

Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico Abstract Few studies have determined the presence of phytoplasmas in important crops in Puerto Rico. Disease symptoms resembling those caused by phytoplasmas were observed in pigeon pea (Cajanus cajan), periwinkle (Catharanthus roseus), tabebuia (Tabebuia heterophylla), Spanish lime (Melicoccus bijugatus), ixora (Ixora coccinea), mango (Mangifera indica), cactus (Opuntia spp.), citrus trees (Citrus spp.), and coffee (Coffea arabica). Sixty-two samples from these species were tested using conventional PCR to amplify the 16S rRNA and ribosomal protein genes (rpIVrpsC). Fifty-one percent of the tested samples (corresponding to periwinkle, pigeon pea, citrus, coffee and tabebuia) were positive for phytoplasmas, with amplicons of 0.8 (16S rRNA gene) and 1.2 kb (rpIV-rpsC genes), depending upon primers used in PCRs. For both genetic loci, DNA sequences showed 99 % identity with pigeon pea witches' broom phytoplasma (PPWB). Due to the lack of studies of potential insect vectors, common Auchenorrhyncha species were sweep-collected from pigeon pea and citrus and tested for phytoplasma. Of nine insect genera collected, Empoasca kraemeri (Cicadellidae), Melormenis antillarum (Flatidae), and Colpoptera maculifrons (Issidae) were positive for PPWB based on results from conventional PCR and DNA sequence analysis. The findings indicate that these insects fed upon the aforementioned plant species, ingesting contents of phloem, and may act as potential phytoplasma vectors in the field. These are first reports of PPWB phytoplasma infections in citrus species (C. sinensis and C. limon), coffee, periwinkle and tabebuia, and in insects (E. kraemeri, M. antillarum and C. maculifrons) for Puerto Rico.

show abstract

Genetic Relatedness Between Two Nonculturable Mycoplasmalike Organisms Revealed by Nucleic Acid Hybridization and Polymerase Chain Reaction

Cited by 159 publications

References 0 publications

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

'Candidatus Phytoplasma castaneae', a novel phytoplasma taxon associated with chestnut witches' broom disease.

Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico

Contact Info

Product

Resources

About