Genetic relatedness between Listeria monocytogenes isolates from seafood and humans using PFGE and REP-PCR

Chou, Chung‐Hsi; Wang, Chinling

doi:10.1016/j.ijfoodmicro.2006.02.003

Cited by 45 publications

(39 citation statements)

References 46 publications

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“…Of interest, it was found that genomic DNA isolates of serotype 4b digested by ApaI enzyme in our study showed relatively distinguishable patterns. This finding is consistent with the study identical PFGE patterns belonged to the same serotype [29] [35].…”

Section: Discussionsupporting

confidence: 93%

Detection of Listeria monocytogenes in Foods and Characterization by PFGE

Park¹,

Jung²,

Lee³

et al. 2016

AiM

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The aims of the present study were to investigate the prevalence of Listeria monocytogenes in 1042 foods collected from different market to characterize the isolates by phenotypical and molecular methods. In particular, L. monocytogenes obtained from different types of foods such as RTE (kimbap), fish (smoked salmon and seasoned-dried slice fish) and meat (cut raw beef and pork) from 2009 to 2011, were used. Twelve samples (2.1%) were positive for L. monocytogenes. Detection rate of L. monocytogenes varied significantly by food type and ranged from 1.1% to 5.2%. Meat is the highest prevalence for L. monocytogenes (5.2%) followed by RTE (1.8%) and Fish (1.1%). Twelve isolates were also serotyped by the agglutination method. The most common serotypes detected in the 12 strains tested were 1/4b (75.0%), followed by 1/2a (16.7%), and 1/2b (8.3%). For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. PFGE was performed to determine genetic characterization of L. monocytogenes strains to define the genetic diversity.

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Section: Discussionsupporting

confidence: 93%

Detection of Listeria monocytogenes in Foods and Characterization by PFGE

Park¹,

Jung²,

Lee³

et al. 2016

AiM

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“…It should be considered also that the rep-PCR method herein applied does not allow to differentiate highly similar strains, given the 95% similarity cut-off chosen for the identification of identical strains. However, research previously published (Chou and Wang, 2006;Zunabovic et al, 2012;Nucera et al, 2013) showed that the discriminatory power of PCR typing is comparable to that of PFGE (the current gold standard typing method for L. monocytogenes). For these reasons, its applicability as a screen typing tool in field research cannot be denied, considering that results on large-scale sampling can be obtained in less time and with the investment of less resources, in comparison to what PFGE would require.…”

Section: Discussionmentioning

confidence: 99%

“…Dairy cows may be directly exposed to L. monocytogenes through the ingestion of improperly fermented silage (pH >5.0) contaminated before ensiling (Fenlon, 1988), and L. monocytogenes may then reach bulk tanks as a result of fecal contamination during milking, as also reported for spore-forming bacteria (Vissers et al, 2007). Other than Listeria detection, currently strain typing has largely been applied to explore subtype frequency and distribution: some authors applied repetitive element sequence-based PCR (REP) for characterizing isolates collected from dairy primary production as well as from the food processing environment (Harvey et al, 2004;Van Kessel et al, 2005;Chou and Wang, 2006), indicating the putative transmission/contamination paths.…”

Section: Introductionmentioning

confidence: 97%

Detection, identification, and typing of Listeria species from baled silages fed to dairy cows

Nucera

Grassi

Morra

et al. 2016

Journal of Dairy Science

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Anaerobiosis, critical for successful ensilage, constitutes a challenge in baled silages. The loss of complete anaerobiosis causes aerobic deterioration and silages undergo dry matter and nutrient losses, pathogen growth, and mycotoxin production. Silage may represent an ideal substrate for Listeria monocytogenes, a pathogen of primary concern in several cheeses. The aim of this research was to investigate the occurrence of Listeria in baled silage fed to cows producing milk for a protected designation of origin cheese, and to characterize isolates by repetitive sequence-based PCR. Listeria spp. were detected in 21 silages and L. monocytogenes in 6 out of 80 of the analyzed silages; 67% of positives were found in molded zones. Results of the PCR typing showed genotypic homogeneity: 72.9 and 78.8% similarity between strains of Listeria spp. (n = 56) and L. monocytogenes (n = 24), respectively. Identical profiles were recovered in molded and nonmolded areas, indicating that contamination may have occurred during production. The application of PCR allowed the unambiguous identification of Listeria isolated from baled silages, and repetitive sequence-based PCR allowed a rapid and effective typing of isolates. Results disclose the potential of the systematic typing of Listeria in primary production, which is needed for the understanding of its transmission pathways.

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“…It was reported that these serotypes were widely distributed in food, animal, and environment of Korea. Chung HC et al indicated that some serotype 1/2 isolates which showed that the H-antigenic factors could not be fully determined by traditional antiserum serotyping [20]. Because most human infections are reported to be associated with 1/2a, 1/2b, and 4b, our results were suggested that three major serotypes may be particularly important.…”

Section: Discussionmentioning

confidence: 64%

“…But, genomic DNA isolates of serotype 4b digested by ApaI enzyme in our study showed relatively distinguishable patterns not including No.45 sample and reference strain s1. It was indicated that identical PFGE patterns belonged to the same serotype [20,41]. The use of both RAPD and PFGE for typing L. monocytogenes has previously been reported to identify similar group and isolates from different sources [11,44].…”

Section: S Park Et Al 614mentioning

confidence: 98%

Molecular Characterization of Listeria monocytogenes Based on the PFGE and RAPD in Korea

Park¹,

Jung²,

Choi³

et al. 2012

AiM

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This study was performed to characterize 35 L. monocytogenes isolates from animals, foods, environmental samples collected between 1997 and 2007 with no apparent epidemiological relations, and five reference isolates using serotypic, genotypic and molecular typing methods to understand the pattern of strain distribution in Korea. For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. We also compared RAPD and PFGE to determine genetic characterization of L. monocytogenes strains to define the genetic diversity. Serotype patterns of the 30 L. monocytogenes strains was as follows; 9 isolates (30.0 %) belonged to serotype, 7 isolates (23.3%) belonged to serotype 4b, 4 isolates (13.3%) belonged to serotype 1/2b, 3 isolates (10.0%) belonged to serotype 1/2c, 2 (6.7%) isolates belonged to 4c, 2 (6.7%) isolates belonged to NT (Non Type), one isolate (3.2%) belonged to 3a and 3b, and 4a, respectively. Although, a limited number of isolates were analyzed in this study, molecular typing with RAPD and PFGE indicated that PFGE is more discriminatory for the subtyping L. monocytogenes than RAPD. Some L. monocytogenes isolates by RAPD and PFGE types are associated with specific sources. And, combining data obtained by these methods will increases the likelihood of strain discrimination.

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Genetic relatedness between Listeria monocytogenes isolates from seafood and humans using PFGE and REP-PCR

Cited by 45 publications

References 46 publications

Detection of <i>Listeria monocytogenes</i> in Foods and Characterization by PFGE

Detection of <i>Listeria monocytogenes</i> in Foods and Characterization by PFGE

Detection, identification, and typing of Listeria species from baled silages fed to dairy cows

Molecular Characterization of <i>Listeria monocytogenes</i> Based on the PFGE and RAPD in Korea

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Genetic relatedness between Listeria monocytogenes isolates from seafood and humans using PFGE and REP-PCR

Cited by 45 publications

References 46 publications

Detection of &lt;i&gt;Listeria monocytogenes&lt;/i&gt; in Foods and Characterization by PFGE

Detection of &lt;i&gt;Listeria monocytogenes&lt;/i&gt; in Foods and Characterization by PFGE

Detection, identification, and typing of Listeria species from baled silages fed to dairy cows

Molecular Characterization of &lt;i&gt;Listeria monocytogenes&lt;/i&gt; Based on the PFGE and RAPD in Korea

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Product

Resources

About

Detection of <i>Listeria monocytogenes</i> in Foods and Characterization by PFGE

Detection of <i>Listeria monocytogenes</i> in Foods and Characterization by PFGE

Molecular Characterization of <i>Listeria monocytogenes</i> Based on the PFGE and RAPD in Korea