Genetic relatedness between group B streptococci originating from bovine mastitis and a human group B streptococcus type V cluster displaying an identical pulsed-field gel electrophoresis pattern

Oliveira, Ivi Cristina Menezes de; Mattos, Marcos Corrêa de; Pinto, T. A.; Ferreira-Carvalho, Bernadete Teixeira; Benchetrit, Leslie C.; Whiting, April; Bohnsack, John F.; Figueiredo, Agnes Marie Sá

doi:10.1111/j.1469-0691.2006.01508.x

Cited by 42 publications

(37 citation statements)

References 39 publications

(66 reference statements)

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“…This study, along with previous studies, indicates that these serotype V GBS are clonal and are from CC 1 (4,5,15,16,23). While colonizing serotype V strains in this study were also occasionally found in a variety of other lineages, the only other invasive serotype V strain in this study was ST-26, an ST related to a bovine serotype V ST-256 strain isolated from a bovine udder in Brazil (27). CC 1 is comprised of the smallest number of STs of the major CCs associated with invasive disease in this study, with 93% of the CC 1 strains having a single ST (ST-1), suggesting that the diversity of STs within a CC may reflect how recently an invasive clone arose in the human population.…”

Section: Discussionsupporting

confidence: 78%

Population Structure of Invasive and Colonizing Strains of Streptococcus agalactiae from Neonates of Six U.S. Academic Centers from 1995 to 1999

Bohnsack¹,

Whiting²,

Gottschalk

et al. 2008

J Clin Microbiol

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The purpose of this study was to describe the population structure of group B streptococci (GBS) isolated from infected and colonized neonates during a prospective active-surveillance study of early-onset disease in six centers in the United States from July 1995 to June 1999 and to examine its relationship to bovine strains of GBS. The phylogenetic lineage of each GBS isolate was determined by multilocus sequence typing, and isolates were clustered into clonal complexes (CCs) using the eBURST software program. A total of 899 neonatal GBS isolates were studied, of which 129 were associated with invasive disease. Serotype Ia, Ib, and V isolates were highly clonal, with 92% to 96% of serotype Ia, Ib, and V isolates being confined to single clonal clusters. In contrast, serotype II and III isolates were each comprised of two major clones, with 39% of serotype II and 41% of serotype III isolates in CC 17 and 41% of serotype II and 54% of serotype III isolates in CC 19. Further analysis demonstrates that the CC 17 serotype II and III GBS are closely related to a previously described "ancestral" lineage of bovine GBS. While 120 (93%) of invasive GBS were confined to the same lineages that colonized neonates, 9 (7%) of the invasive GBS isolates were from rare lineages that comprised only 2.7% of colonizing lineages. These results are consistent with those for other geographic regions that demonstrate the highly clonal nature of GBS infecting and colonizing human neonates.Streptococcus agalactiae (group B streptococci [GBS]), a primary cause of bovine mastitis, emerged in the 1960s as a significant human pathogen that causes neonatal sepsis and meningitis (1). Despite a recent decline in incidence, GBS remains a leading cause of neonatal mortality and morbidity in the United States (2,7,29,30). GBS colonizes the genitourinary and gastrointestinal tracts of about a third of healthy adult women (24). Neonatal disease results from transmission of the organism from the pregnant mother to the neonate. Heavy colonization of the neonate and maternal chorioamnionitis are known risk factors for neonatal infection (10,20).GBS can be divided into nine serotypes based on the immunologic reactivity of the capsular polysaccharide. The structure of GBS capsular polysaccharide is determined by genes in the cps locus which encode enzymes responsible for the synthesis of the polysaccharides (8,34,35). Investigators using a variety of techniques have demonstrated that the population of GBS that colonizes and infects humans is comprised of a relatively small number of lineages (4, 12-16, 23, 26, 28, 31, 32). Each serotype is usually distributed among several different lineages, and each lineage may contain several different serotypes, indicating that serotype alone is not a sufficient marker to identify a phylogenetic lineage and suggesting that serotype switching occurs within lineages, presumably by horizontal transfer of cps genes (4,16,23).Multilocus sequence typing (MLST) has become the standard method for determining the population s...

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Section: Discussionsupporting

confidence: 78%

Population Structure of Invasive and Colonizing Strains of Streptococcus agalactiae from Neonates of Six U.S. Academic Centers from 1995 to 1999

Bohnsack¹,

Whiting²,

Gottschalk

et al. 2008

J Clin Microbiol

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“…Gels were stained with ethidium bromide and photographed under UV light. Criteria for analysis of the PFGE patterns were those originally suggested by Tenover et al (1995) and used in our previous studies (Oliveira et al 2005(Oliveira et al , 2006. PCR -DNA extraction was performed according to Sambrook et al (1989).…”

Section: Methodsmentioning

confidence: 99%

Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil

Corrêa

Oliveira

Pinto

et al. 2009

Mem. Inst. Oswaldo Cruz

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Group B streptococci (GBS) infections occur worldwide. Although serotyping has been used for epidemiologic purposes, this does not accurately characterize enough members of a genetically heterogeneous bacterial population. The aims of this work were to evaluate the genetic diversity of 45 type Ia GBS strains isolated in Brazil byGroup B streptococci (GBS) are responsible for a large variety of human infections and have been recognized over the last few decades as a leading cause of perinatal disease worldwide (Farley 2001, Weisner et al. 2004. GBS are classified in serotypes (Ia, Ib and II-VIII) that occur in combination with different protein antigens (alpha, beta and rib) (Dmitriev et al. 2001). Currently, serotypes Ia, III and V are the most common in many countries (Duarte et al. 2005, Fluegge et al. 2005, Bergseng et al. 2008, Poyart et al. 2008, von Both et al. 2008.Pulsed-field gel electrophoresis (PFGE), used to examine GBS strains, is a powerful technique employed for the classification of microorganisms after digestion of the genomic DNA by restriction enzymes (Benson & Ferrieri 2001, Oliveira et al. 2005, von Both et al. 2008. Using polymerase chain reaction (PCR), Franken et al. (2001) suggested that the scpB and lmb genes (encoding c5a peptidase and laminin binding protein) must exist in GBS strains that infect humans. The hylB gene is frequently detected in these strains (Dore et al. 2003). The bac and bca genes are present in 23% and 100% of type Ia strains, respectively (Maeland et al. 1997(Maeland et al. , 2000.The lack of information on Ia Brazilian strains and the availability of DNA techniques led us to investigate the genetic make-up of type Ia GBS strains isolated in distinct regions of Brazil. Additionally, antimicrobial susceptibility was examined to better characterize these isolates. MATERIALS AND METHODSBacterial strains -Forty-five human type Ia GBS strains derived from clinical specimens were obtained in Florianopolis, Santa Catarina (n = 3), a city located in the Southern Region of Brazil, in São Paulo, São Paulo (SP) (n = 1), and in Rio de Janeiro, Rio de Janeiro (n = 40), in the Southeast Region of the country. The source of one strain was unknown.Isolates were obtained from 1981-2002 from public health laboratories, gynaecological clinics, hospitalsand universities. The clinical sources included urine (n = 16), oropharynx (n = 9), vagina (n = 5), anus (n = 2), lung (n = 2), placenta (n = 1), external ear canal (n = 1) and perineum (n = 1). The sources of eight specimens were unknown. A confirmatory identification of serotypes was carried out again by immunoprecipitation in agarose using sera produced in the research facilities of the authors and HCl antigenic extracts from the streptococci (Benchetrit et al. 1982).PFGE -PFGE was performed as previously described (Oliveira et al. 2005). DNA was digested with the SmaI restriction enzyme (Amersham) and submitted to electrophoresis with a program as follows: switch time of 1-30 sec during 23 h, with a 120º angle, at a temperature of ...

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“…Ideally, assessment of the potential for interspecies transmission is based on the analysis of contemporaneous, sympatric isolates. With 1 exception ( 13 ), however, most comparative studies were not based on isolates from the same geographic region and the same period, or, if they were, they covered a very limited number of S. agalactiae– positive farms or animals ( 14 , 15 ). Our aim with this study was to provide insight into the hazard of interspecies transmission of S. agalactiae by comparing contemporaneous populations of S. agalactiae from humans and dairy cattle in Finland and Sweden.…”

mentioning

confidence: 99%

Streptococcus agalactiae Serotype IV in Humans and Cattle, Northern Europe1

Lyhs¹,

Kulkas²,

Katholm³

et al. 2016

Emerg. Infect. Dis.

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Genetic relatedness between group B streptococci originating from bovine mastitis and a human group B streptococcus type V cluster displaying an identical pulsed-field gel electrophoresis pattern

Cited by 42 publications

References 39 publications

Population Structure of Invasive and Colonizing Strains of Streptococcus agalactiae from Neonates of Six U.S. Academic Centers from 1995 to 1999

Population Structure of Invasive and Colonizing Strains of Streptococcus agalactiae from Neonates of Six U.S. Academic Centers from 1995 to 1999

Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil

Streptococcus agalactiae Serotype IV in Humans and Cattle, Northern Europe1

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