Genetic relatedness and genetic diversity of ornamental mei (Prunus mume Sieb. et Zucc.) as analysed by AFLP markers

Yang, Chaodong; Zhang, Junwei; Yan, Xiao-lan; Bao, Manzhu

doi:10.1007/s11295-007-0106-0

Cited by 11 publications

(7 citation statements)

References 22 publications

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“…bungo) consisting of the hybrids of mei and apricot [1]. Our results confirmed the findings of previous studies regarding the hybrid nature of ‘Dan Fenghou’ and ‘Fen Hou’ using random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers [3,33]. Clade III was found to include plum, indicating a relatively distant interspecies relationship between plum and mei.…”

Section: Resultssupporting

confidence: 89%

“…This is mainly because mei is a woody perennial that takes a long time to reach its reproductive age. Recently, DNA markers have been used to analyze genetic diversity, distinguish varieties, and construct genetic maps [3-6]. However, quantitative trait locus (QTL) analysis, genome-wide association studies (GWAS), and genomic selection studies are impeded due to the limited availability of sufficient DNA markers.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide DNA polymorphisms in two cultivars of mei (Prunus mumesieb. et zucc.)

Sun

Zhang

et al. 2013

BMC Genet

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BackgroundMei (Prunus mume Sieb. et Zucc.) is a famous ornamental plant and fruit crop grown in East Asian countries. Limited genetic resources, especially molecular markers, have hindered the progress of mei breeding projects. Here, we performed low-depth whole-genome sequencing of Prunus mume ‘Fenban’ and Prunus mume ‘Kouzi Yudie’ to identify high-quality polymorphic markers between the two cultivars on a large scale.ResultsA total of 1464.1 Mb and 1422.1 Mb of ‘Fenban’ and ‘Kouzi Yudie’ sequencing data were uniquely mapped to the mei reference genome with about 6-fold coverage, respectively. We detected a large number of putative polymorphic markers from the 196.9 Mb of sequencing data shared by the two cultivars, which together contained 200,627 SNPs, 4,900 InDels, and 7,063 SSRs. Among these markers, 38,773 SNPs, 174 InDels, and 418 SSRs were distributed in the 22.4 Mb CDS region, and 63.0% of these marker-containing CDS sequences were assigned to GO terms. Subsequently, 670 selected SNPs were validated using an Agilent’s SureSelect solution phase hybridization assay. A subset of 599 SNPs was used to assess the genetic similarity of a panel of mei germplasm samples and a plum (P. salicina) cultivar, producing a set of informative diversity data. We also analyzed the frequency and distribution of detected InDels and SSRs in mei genome and validated their usefulness as DNA markers. These markers were successfully amplified in the cultivars and in their segregating progeny.ConclusionsA large set of high-quality polymorphic SNPs, InDels, and SSRs were identified in parallel between ‘Fenban’ and ‘Kouzi Yudie’ using low-depth whole-genome sequencing. The study presents extensive data on these polymorphic markers, which can be useful for constructing high-resolution genetic maps, performing genome-wide association studies, and designing genomic selection strategies in mei.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Genome-wide DNA polymorphisms in two cultivars of mei (Prunus mumesieb. et zucc.)

Sun

Zhang

et al. 2013

BMC Genet

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“…Fang et al [4] developed a set of molecular markers, such as AFLP and SNP, to investigate the genetic relatedness and diversity of 50 cultivars of fruiting mei from China and Japan. Similar work using AFLP markers was conducted by Yang et al [5] to analyze the genetic diversity of ornamental mei and compare it with that of other related species. Li et al [6] developed more informative multiallelic microsatellite markers, i.e., simple sequence repeats (SSRs), from 20 mei plants, particularly useful to study the genetic structure of natural populations in mei.…”

Section: Introductionmentioning

confidence: 90%

Genetic control of juvenile growth and botanical architecture in an ornamental woody plant, Prunus mumeSieb. et Zucc. as revealed by a high-density linkage map

Sun

Wang

Yan³

et al. 2014

BMC Genet

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Mei, Prunus mume Sieb. et Zucc., is an ornamental plant popular in East Asia and, as an important member of genus Prunus, has played a pivotal role in systematic studies of the Rosaceae. However, the genetic architecture of botanical traits in this species remains elusive. This paper represents the first genome-wide mapping study of quantitative trait loci (QTLs) that affect stem growth and form, leaf morphology and leaf anatomy in an intraspecific cross derived from two different mei cultivars. Genetic mapping based on a high-density linkage map constricted from 120 SSRs and 1,484 SNPs led to the detection of multiple QTLs for each trait, some of which exert pleiotropic effects on correlative traits. Each QTL explains 3-12% of the phenotypic variance. Several leaf size traits were found to share common QTLs, whereas growth-related traits and plant form traits might be controlled by a different set of QTLs. Our findings provide unique insights into the genetic control of tree growth and architecture in mei and help to develop an efficient breeding program for selecting superior mei cultivars.

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“…Diagnostic AFLP loci along with targeted SSR markers provided more insight on potential origin and breeding history of the PPV-resistant North American apricots . AFLP markers were successfully applied for germplasm analysis in Japanese apricot ( P. mume ) and in relation to their origin and dissemination from the southwest of China (Yang et al 2008 ) . So far, six of seven published genetic linkage maps were saturated with AFLP markers (Table 12.3 ).…”

Section: Molecular Markers Available For Breeding In Apricotmentioning

confidence: 99%

Apricot

et al. 2011

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Apricot is in the Rosaceae family within the genus Prunus L., subgenus Prunophora Focke, and the section Armeniaca (Lam.) Koch. Depending on the classifi cation system, the number of apricot species ranges from 3 to 12. Six distinct species are usually recognized: P . brigantina Vill., P . holosericeae Batal, P . armeniaca L., P . mandshurica (Maxim), P . sibirica L., Japanese apricot P . mume (Sieb.) Sieb. & Succ. Vavilov placed apricot in three centers of origin: the Chinese center (Central and Western China), the Central Asiatic center (Afghanistan, northwest India and Pakistan, Kashmir, Tajikistan, Uzbekistan, Xinjing province in China and western Tien-Shan), and the Near-Eastern center (interior of Asia Minor). Kostina further divided the cultivated apricot according to their adaptability into four major ecogeographical groups : (1) the Central Asian group, (2) the Iran-Caucasian group, (3) the European group, and (4) the Dzhungar-Zailij group. Many local cultivars are grown in the different areas and producing countries; however, these cultivars lack important traits that needed by modern production and marketing systems. Breeding programs have and continue to develop cultivars with improved adaptability to the environment (temperature requirements, water defi cit), extension of the harvest season, fruit quality for fresh consumption and processing, productivity, adequate tree size, and resistance to biotic stresses. The major objectives in apricot breeding

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Genetic relatedness and genetic diversity of ornamental mei (Prunus mume Sieb. et Zucc.) as analysed by AFLP markers

Cited by 11 publications

References 22 publications

Genome-wide DNA polymorphisms in two cultivars of mei (Prunus mumesieb. et zucc.)

Genome-wide DNA polymorphisms in two cultivars of mei (Prunus mumesieb. et zucc.)

Genetic control of juvenile growth and botanical architecture in an ornamental woody plant, Prunus mumeSieb. et Zucc. as revealed by a high-density linkage map

Apricot

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