Genetic regulation of the catalase developmental program in maize scutellum: Identification of a temporal regulatory gene

Scandalios, John G.; Chang, Daniel; McMillin, David E.; Tsaftaris, Athanasios; Moll, R. H.

doi:10.1073/pnas.77.9.5360

Cited by 55 publications

(44 citation statements)

References 21 publications

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“…However, there is an accumulating body of evidence from other systems that temporal genes can act through diffusible regulatory substances that are not products of the enzyme structural gene that is being programed. The best characterized examples concern mouse a-galactosidase (26,27), mouse -galactosidase (3), Drosophila amylase (6), maize alcohol dehydrogenase (28), and maize catalase (29); the developmental programs of these proteins are determined by temporal genes located at some distance from the structural genes for these enzymes. Given this evidence for separate regulatory substances, one possible explanation of Gust action in particular suggests itself.…”

Section: Resultsmentioning

confidence: 99%

Trans-acting temporal locus within the beta-glucuronidase gene complex.

Lusis¹,

Chapman²,

Wangenstein³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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Mice carrying the [Gus]' haplotype of the Pglucuronidase gene complex have considerably decreased enzyme levels and a decreased rate of enzyme synthesis. This is now shown to result from the action of two regulatory loci within the gene complex. One is a systemic regulator, Gus-u, that acts cis to cause a uniform reduction in enzyme levels in all tissues. The other is a temporal locus, Gus-t, that acts trans to cause abrupt switches in the rate of enzyme synthesis in only certain tissues and at characteristic stages of development. The distinction between these two loci was made possible by the introduction of a method for quantitating the relative numbers of A and H allozyme subunits in (-glucuronidase tetramers. The procedure involves purification of the enzyme, cleavage at methionyl residues with CNBr, isoelectric focusing to separate the peptides, and quantitation of the peptide containing the A/H amino acid substitution. The presence of a tram-acting regulatory locus within a gene complex raises evolutionary and functional questions about why it is located there and how it acts.

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Section: Resultsmentioning

confidence: 99%

Trans-acting temporal locus within the beta-glucuronidase gene complex.

Lusis¹,

Chapman²,

Wangenstein³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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“…Cat2 is expressed predominantly in the scutellum during postgerminative sporophytic development and in green leaves (1). The temporal and spatial expression of the catalase structural genes in maize is influenced by genetic elements (2)(3)(4)(5) and environmental signals (6). We have demonstrated (6) that a dramatic induction of the CAT-2 isozyme occurs in maize leaves when dark-grown seedlings are exposed to white light.…”

mentioning

confidence: 92%

“…W64A seedlings were cultured as described (3). The seedlings were grown in a dark growth chamber at 250C for 8 days from the end of imbibition (unless otherwise specified in figure legends).…”

mentioning

confidence: 99%

Translational control of photo-induced expression of the Cat2 catalase gene during leaf development in maize.

Skadsen¹,

Scandalios²

1987

Proc. Natl. Acad. Sci. U.S.A.

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In dark-grown leaves of Zea mays, the catalase (H2O2:H2O2 oxidoreductase; EC 1.11.1.6; CAT) isozyme CAT-2 is absent. With continuous white light, CAT-2 protein levels increase (due to de novo synthesis) and plateau after 24 hr. When total poly(A)+ RNA (mRNA), polysomes, or isolated polysomal mRNA from light- and dark-treated leaves was translated in vitro, CAT-2 was detected only among the light-treated leaf products. A Cat2 clone was used to probe blots of total mRNA and polysomal mRNA from light- and dark-treated leaves. Cat2 mRNA was found in approximately equal quantities in both. Cat2 mRNA was equally distributed in identical high molecular weight fractions in polysomes from light- and dark-treated leaves and, therefore, was probably not sequestered in ribonucleoprotein particles in dark-grown leaves. The control of Cat2 expression appears to involve a unique form of translational inhibition in dark-grown leaves, preventing translation of the isolated mRNA and preventing polypeptide elongation. These results may have important implication in studies of translational control in other systems.

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“…12334 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, North Carolina. 2 Present address: U.S. Department of Agriculture, Agricultural Research Service, Oxford, NC 27565. lum (5,18). Zymogram analysis showed that CAT-I is found primarily in the milky endosperm of the developing kernel.…”

mentioning

confidence: 99%

The Distribution of Catalase Activity, Isozyme Protein, and Transcript in the Tissues of the Developing Maize Seedling

Redinbaugh¹,

Sabre²,

Scandalios³

1990

Plant Physiol.

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The catalase activity, CAT-2 and CAT-3 isozyme protein levels, and the steady-state mRNA levels for each of the three catalase genes were determined in the scutellum, root, epicotyl, and leaf of the developing maize (Zea mays L.) seedling. Catalase activity was highest in the scutellum, with 10-fold lower enzyme activity in the leaf and epicotyl. Very low levels of catalase activity were found in the root. The highest levels of CAT-2 protein were found in the scutellum, with about 10-fold lower levels in the green leaf.

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Genetic regulation of the catalase developmental program in maize scutellum: Identification of a temporal regulatory gene

Cited by 55 publications

References 21 publications

Trans-acting temporal locus within the beta-glucuronidase gene complex.

Trans-acting temporal locus within the beta-glucuronidase gene complex.

Translational control of photo-induced expression of the Cat2 catalase gene during leaf development in maize.

The Distribution of Catalase Activity, Isozyme Protein, and Transcript in the Tissues of the Developing Maize Seedling

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