Genetic redundancy and persistence of plasmid-mediated trimethoprim/sulfamethoxazole resistant effluent and stream water Escherichia coli

Suhartono, Suhartono; Savin, Mary C.; Gbur, Edward E.

doi:10.1016/j.watres.2016.07.035

Cited by 16 publications

(10 citation statements)

References 45 publications

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“…Whereas, the relative abundance of sul2 was negatively correlated with SDZ and SM1 (Figure 6E), Huang et al (2017) indicated that sul 2 was significant correlated to intI 1 in water and sediment samples, which meant that sul 2 gene were associated with the mobile genetic elements (Cheng et al, 2013). Researchers also found that sul2 and sul3 located on plasmids are transferable and stable even in the absence of antibiotics (Suhartono et al, 2016). Thus, this negative correlation between sul2 and residue of sulfonamides might be attributed to mobility of sul2 located on mobile genetic elements.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Bacterial Community Characteristics, Abundance of Antibiotics and Antibiotic Resistance Genes Along a Pollution Gradient of Ba River in Xi’an, China

Guan

Jia

et al. 2018

Front. Microbiol.

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The microbial communities in freshwater have raised concerns about the ecosystem and human health. Many ecological environmental problems have been found in urban river because of the unreasonable use and long-term wastewater discharge. In this study, we explored the bacterial community composition, abundance of 14 antibiotics and 21 antibiotic resistance genes (ARGs), and water environment features in seven water samples and seven sediment samples from Ba River in Xi’an, China. Results showed Proteobacteria and Bacteroidetes were the dominant phyla in all samples, and sediment samples had a higher bacterial diversity and richness than it in water. Bacterial communities of site 5 and 6 were clustered in discrepant patterns compared to those at remaining sites from other samples. It might be influenced by nutrients, heavy metals and antibiotics. Antibiotics concentrations ranged from 1.26 to 1.61 × 103 ng L-1 in water samples and 1.55 to 4.05 × 102 μg kg-1 in sediment samples. Sulfamerazine (SM1) and erythromycin (ERY) were the chief antibiotics in water samples, while the level of oxytetracycline (OTC) and cefazolin (CFZ) were higher in sediment samples. Canonical correspondence analysis showed that trimethoprim (TMP) was significantly related to Acinetobacter in W6, and that SM1 and OTC had positive correlation with Arcobacter in W5. The tetC, blaTEM, ermF and sul1 had higher pollution abundance ranging from 10-4 to 100 copies/16S rRNA gene copies in all samples. Significant correlations were observed between ARGs and matching antibiotics, suggesting that antibiotics can pose the selective pressure on ARGs in this river. In summary, these finding might provide some new data to the limited information available on the bacterial community characteristics, abundance of antibiotics and ARGs in urban river of China.

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Section: Discussionmentioning

confidence: 99%

Analysis of Bacterial Community Characteristics, Abundance of Antibiotics and Antibiotic Resistance Genes Along a Pollution Gradient of Ba River in Xi’an, China

Guan

Jia

et al. 2018

Front. Microbiol.

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“…The genes sul2, sul3, tet(A) and vanA were not detected in any water samples across the three communities. This is despite them being commonly detected in various environments (Suhartono, Savin and Gbur 2016;Young et al 2016;Zhang, Lin and Yu 2016). Three genes, ampC, mecA and sul1 were detected in a few water samples (Fig.…”

Section: Detection Of Argsmentioning

confidence: 96%

“…Sulfonamides are one of the earliest antibiotics introduced in the world and widely used for treating human infections (van Hoek et al 2011;Roberts, Schwarz and Aarts 2012). These genes had been commonly detected in various environments such as drinking water, surface water and wastewater (Schwartz et al 2003;Alexander et al 2015;Suhartono, Savin and Gbur 2016). However, even though only a fraction of the samples tested via qPCR were positive for the ARGs, the numbers detected are alarmingly high.…”

Section: Detection Of Argsmentioning

confidence: 99%

Detection of fecal bacteria and antibiotic resistance genes in drinking water collected from three First Nations communities in Manitoba, Canada

Patidar

Farenhorst

et al. 2019

FEMS Microbiology Letters

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This study analyzed the microbiological quality of drinking and source water from three First Nations communities in Manitoba, Canada that vary with respect to the source, storage and distribution of drinking water. Community A relies on an aquifer and Community B on a lake as source water to their water treatment plants. Community C does not have a water treatment plant and uses well water. Quantification of free residual chlorine and fecal bacterial (E. coli and coliforms), as well as detection of antibiotic resistance genes (sul, ampC, tet(A), mecA, vanA, blaSHV, blaTEM, blaCTX-M, blaOXA-1, blaCYM-2, blaKPC, blaOXA-48, blaNDM, blaVIM, blaGES and blaIMP) was carried out. While water treatment plants were found to be working properly, as post-treatment water did not contain E. coli or coliforms, once water entered the distribution system, a decline in the chlorine concentration with a concomitant increase in bacterial counts was observed. In particular, water samples from cisterns not only contained high number of E. coli and coliforms, but were also found to contain antibiotic resistance genes. This work shows that proper maintenance of the distribution and storage systems in First Nations communities is essential in order to provide access to clean and safe drinking water.

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“…27,28 Despite the fact that antibiotics do not significantly influence conjugation within bacteria, 29 antibiotics, at the subinhibitory level, may play an important role in the survival and persistence of antibiotic resistant bacteria in the aquatic environment. 17 In terms of sulfamethoxazole resistance, the number of sul genes in plasmids within an isolate had a statistically significant effect on the transfer frequencies of plasmids based on the ratio of T/D ( genes was intermediate, and not different from, those conferring one or 3 sul genes. Based on the T/R ratio, however, sul gene combination had no significant effects on the transfer frequency ( Fig.…”

Section: ¡5mentioning

confidence: 94%

“…15,16 A subset of isolates expressing resistance to sulfamethoxazole and trimethoprim were found to possess redundancy and a diversity among sulfamethoxazole resistance (sul) or trimethoprim resistance (dfr) genes, respectively. 17 These sul and dfr and other antibiotic resistance genes might be associated with conjugative and/or mobilizable plasmids and integrons, which were also found to be abundant among resistant isolates, 18 promoting dissemination of the antibiotic resistance determinants. Hence, the objective of the research was to determine the differences among sampling site of origin, intI presence, phenotypic resistance to number of different antibiotics, and number of sulfamethoxazole resistance (sul) or trimethoprim resistance (dfr) genes with respect to transfer frequencies measured among isolates.…”

Section: -13mentioning

confidence: 99%

Conjugative transmission of antibiotic-resistance from stream waterEscherichia colias related to number of sulfamethoxazole but not class 1 and 2 integrase genes

Suhartono

Savin

2016

Mobile Genetic Elements

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A conjugation assay was used to determine the effects of phenotypic resistance to one to up to 5 antibiotics, sampling site of origin, presence or absence of class 1 and/or class 2 integrase (intI) genes (intI1 and intI2), and the number of sulfamethoxazole resistance (sul) and trimethoprim resistance (dfr) genes on the transfer frequencies of plasmids from environmental, antibioticresistant Escherichia coli. Of 51 sulfamethoxazole and trimethoprim-resistant E. coli isolates conferring at least one mob gene (mob P51 , mob F11 , mob F12 , mob Q11 , mob Q12 , or mob Qu ), 38 produced transconjugants with an overall mean frequency of 1.60 £ 10 ¡3 transconjugants/ donors (T/D) or 5.89 £ 10 ¡3 transconjugants/recipients (T/R). The presence or absence of intI1 and intI2 and the presence or absence of different targeted dfr genes (dfrA1, dfrA8, dfrA12, dfrA14, dfrA17, and/or dfrB3) were not statistically related to plasmid transfer frequencies as determined by ANOVA (P 0.05). However, E. coli isolates recovered 2 km downstream of wastewater treatment plant effluent input, and those possessing resistance to 3 antibiotics had significantly greater plasmid transfer frequency than their counterparts when calculated as T/D (ANOVA followed by Fisher's least significant difference means comparison, P < 0.05). Greater plasmid transfer frequency calculated as T/D was also measured for E. coli possessing 3 compared to a single sul gene. The invitro frequency suggests that horizontal gene transfer of conjugative mediated-antibiotic (sul) resistance genes may be significant among resistant, stream bacteria.

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Genetic redundancy and persistence of plasmid-mediated trimethoprim/sulfamethoxazole resistant effluent and stream water Escherichia coli

Cited by 16 publications

References 45 publications

Analysis of Bacterial Community Characteristics, Abundance of Antibiotics and Antibiotic Resistance Genes Along a Pollution Gradient of Ba River in Xi’an, China

Analysis of Bacterial Community Characteristics, Abundance of Antibiotics and Antibiotic Resistance Genes Along a Pollution Gradient of Ba River in Xi’an, China

Detection of fecal bacteria and antibiotic resistance genes in drinking water collected from three First Nations communities in Manitoba, Canada

Conjugative transmission of antibiotic-resistance from stream waterEscherichia colias related to number of sulfamethoxazole but not class 1 and 2 integrase genes

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