Genetic recombination in the major histocompatibility complex (H-1, Ag-B) of the rat

Monoclonal Antibodies as Tools to Analyze the Serological and Genetic Complexities of Major Transplantation Antigens

“…Some preliminary data have been published elsewhere (Galfre et al 1977, Howard et al 1978. ARC Ganther & Stark 1977Gasser 1977 .lW Stark et al 1977 ' A nomenclature for MHC hapiotypes of laboratory rat was agreed at the 2nd International Workshop on Alloantigenic Systems in tbe Rat, Freiburg, May 14-16 1979, under…”

At Least Two Loci of the Major Histocompatibility Complex Can Determine Mixed Lymphocyte Stimulation in the Rat

“…1977) and LEW. l A R l have been developed from the recombinant rat reported on previously (Stark et al 1977). Rats used were 3 to 6 months old and sex-matched in the same experiment.…”

“…By this recombinant, called H-lar', the MHC of the rat was divided into two regions, A and B, the latter including the Ir gene cluster and possibly being the homologue of the mouse H-21 region (Gunther et al 1978). Preliminary MLR analysis of this recombinant has reveaIed that major MLR genes were recombined together with the Ir genes and, in addition, suggested that there might be a second weak MLR-determining gene (Stark et al 1977). This preliminary conclusion had to remain unconfirmed since no homozygous H-lar' carrying cells were available.…”

Properties of Rat Anti‐MHC Antibodies Produced by Cloned Rat‐Mouse Hybridomas

“…Second, the MHC of rats is not as well defined as its H-2 counterpart in the mouse. In part this is due to a lack of MHC recombinant strains of the rat (Butcher & Howard 1977, Stark et al 1977, a difficulty which can be overcome in part by the availability of a panel of anti-rat MHC hybridomas. The available amino acid sequence data indicate that the MHC products of the rat, the mouse and humans display regions of homology (Blankenhorn et al 1978) which presumably account for the observed serological cross-reactivities occasionally seen with alloantisera directed against MHC antigens (Sachs et al 1977, Shinohara et al 1978.…”